A5088163862- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cervical Cancer and HPV Research
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- HIV Research and Treatment
- Immune Response and Inflammation
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Cancer Research and Treatments
- SARS-CoV-2 and COVID-19 Research
- Reproductive System and Pregnancy
- Glycosylation and Glycoproteins Research
- Animal Virus Infections Studies
- Influenza Virus Research Studies
- Mesenchymal stem cell research
- Renal and related cancers
- Cancer-related Molecular Pathways
- HIV/AIDS drug development and treatment
- Enzyme Production and Characterization
- Chemokine receptors and signaling
Pohang University of Science and Technology
2015-2024
Genexine (South Korea)
2015-2024
KoreaBio
2022
Seoul St. Mary's Hospital
2012
Catholic University of Korea
2012
Seoul National University
2011
Garvan Institute of Medical Research
2011
Soonchunhyang University
2011
Dong Wha Pharm (South Korea)
2001
Hanyang University
2001
Abstract Here, we demonstrate that electroporation-enhanced immunization with a rationally designed HPV DNA vaccine (GX-188E), preferentially targeting antigens to dendritic cells, elicits significant E6/E7-specific IFN-γ-producing T-cell response in all nine cervical intraepithelial neoplasia 3 (CIN3) patients. Importantly, eight out of patients exhibit an enhanced polyfunctional HPV-specific CD8 as shown by increase cytolytic activity, proliferative capacity and secretion effector...
DNA transcription is initiated by a small regulatory region of transactivators known as the transactivation domain. In contrast to rapid progress made on functional aspect this promiscuous domain, its structural feature still poorly characterized. Here, our multidimensional NMR study reveals that an unbound full-length p53 although similar recently discovered group loosely folded proteins in it does not have tertiary structure, nevertheless populated amphipathic helix and two nascent turns....
Clinical trials of gene therapy using a viral delivery system for glioma have been limited. Recently, stem cells as the vehicles therapeutic agents has emerged new treatment strategy malignant brain tumors. In this study, we used human umbilical cord blood–derived mesenchymal (UCB-MSCs) with glioma-targeting capabilities, and modified interleukin-12 (IL-12p40N220Q; IL-12M) novel gene. We also engineered UCB-MSCs to secret IL-12M (UCB-MSC-IL12M) via tetrameric cell-permeable peptide...
Owing to its tumor tropism and prolonged transgene expression, mesenchymal stem cell (MSC) has been considered as an ideal delivery vehicle for cancer gene therapies or therapeutic vaccines. In this study, we demonstrated that intratumoral (i.t.) injection of MSCs expressing modified interleukin-12 (MSCs/IL-12M) exhibited stronger tumor-specific T-cell responses antitumor effects well more sustained expressions IL-12 interferon (IFN)-γ in both sera sites than did IL-12M-expressing adenovirus...
Abstract Purpose: To determine the efficacy of therapeutic DNA vaccine GX-188E for inducing regression cervical intraepithelial neoplasia (CIN) 3. Patients and Methods: We conducted a prospective, randomized, multicenter, open-label, phase II clinical trial in CIN3 patients positive human papillomavirus (HPV) type 16/18. The primary endpoint was to histopathologic ≤CIN1 at visit seven (V7; 20 weeks after first injection), an extension study pursued until 8 (V8; 36 injection). HPV-sequencing...
We assessed the safety and immunogenicity of two recombinant DNA vaccines for COVID-19: GX-19 containing plasmid encoding SARS-CoV-2 spike protein, GX-19N receptor-binding domain (RBD) foldon, nucleocapsid protein.Two open-label non-randomised phase 1 trials, one other were done at hospitals in South Korea. enrolled healthy adults aged 19-49 years trial 19-54 trial. Participants who tested positive by serological testing excluded. At 4-week intervals, participants received vaccine doses...
ABSTRACT Persistent infection with hepatitis C virus (HCV) is associated the development of liver cirrhosis and hepatocellular carcinoma. To examine oncogenic potential HCV core gene product, primary rat embryo fibroblasts (REFs) were transfected in presence or absence H- ras oncogene. In contrast to a previous report (R. B. Ray, L. M. Lagging, K. Meyer, R. J. Virol. 70:4438–4443, 1996), proteins from two different genotypes (type 1a type 1b) not found transform REFs tumorigenic phenotype...
ABSTRACT T follicular helper (Tfh) cells are specialized providers of cognate B cell help, which is important in promoting the induction high-affinity antibody production germinal centers (GCs). Interleukin-6 (IL-6) and IL-21 have been known to play roles Tfh differentiation. Here, we demonstrate that IL-7 plays a pivotal role generation GC formation vivo , as treatment with anti-IL-7 neutralizing markedly impaired development IgG responses. Moreover, codelivery mouse Fc-fused (IL-7-mFc)...
Synthetic hydrogels have been extensively investigated as artificial extracellular matrices (ECMs) for tissue engineering in vitro and vivo. Crucial challenges such are sustaining long‐term cytocompatible encapsulation providing appropriate cues at the right place time spatio‐temporal control of cells. Here, situ supramolecularly assembled modularly modified engineered mesenchymal stem cell (eMSC) therapy reported using cucurbit[6]uril‐conjugated hyaluronic acid (CB[6]‐HA), diaminohexane...
The cytokine IL-7 plays critical and nonredundant roles in T cell immunity so that the abundance availability of act as key regulatory mechanisms immunity. Importantly, is not produced by cells themselves but primarily non-lymphoid lineage stromal epithelial are limited their numbers. Thus, depend on extrinsic IL-7, amount
Tumor suppressor protein p53 is a potent transcriptional activator and regulates cell growth negatively. To characterize the activation domain (TAD) of p53, various point mutants were constructed in context Gal4 DNA binding tested for their transactivation ability. Our results demonstrated that positionally conserved hydrophobic residues shared with herpes simplex virus VP16 other transactivators are essential transactivation. Also, negatively charged proline necessary full activity, but not...
Abstract IL-12 is a pleiotropic cytokine that plays an important role in innate and adaptive immunity. induces T cell proliferation IFN-γ secretion from activated cells. It was also reported prevents apoptosis of CD4+ However, the signaling mechanism regulates these IL-12-induced responses poorly understood yet. In this study, we demonstrated activates phosphatidylinositol 3-kinase (PI3K)/Akt pathway murine cells, required for antiapoptotic function, but not induction. Through PI3K/Akt...
Liver cirrhosis is one of the major complications hepatitis C virus (HCV) infection, but mechanisms underlying HCV-related fibrogenesis are still not clear. Although roles HCV core protein remain poorly understood, it supposed to play an important role in regulation cellular growth and hepatocarcinogenesis. The aim this study was examine on hepatic fibrogenesis. We established vitro co-culture system with primary stellate cell (HSC) isolated from rats, a stable HepG2-HCV line which had been...
IL-12p70 plays a pivotal role in regulating the Th1/Th2 balance initial stage of immune responses. In contrast, IL-12p40, which is produced excess over IL-12p70, has been known to down-regulate IL-12p70-mediated responses by acting as an antagonist. To investigate vivo function RH7777 rat hepatoma cells were engineered inducibly express mouse IL-12p40 under tight control doxycycline (dox). absence dox, s.c. injection these into syngeneic was shown generate tumors. However, induction dox...
Abstract DNA immunization induces CD8+ CTL responses by bone marrow-derived APCs, which are directly transfected with a plasmid and/or acquire Ags from DNA-transfected non-APCs. To investigate the relative contribution of APCs vs non-APCs to initiation T cell responses, we used tissue-specific promoter-directed gene expression and adoptive transfer systems in gun immunization. In this study, demonstrated that non-APC-specific expressions induced significant IFN-γ-producing cells Ab whereas...
Abstract CpG DNA has been recognized as a powerful stimulant of dendritic cells (DCs). In this study, we demonstrate that inhibits spontaneous apoptosis DCs. up-regulated cellular inhibitor proteins (cIAPs) well Bcl-2 and Bcl-xL, but down-regulated active caspase-3. Although activated p38 mitogen-activated protein kinase, extracellular signal-related phosphatidylinositide-3′-OH kinase (PI3K), only the blocking PI3K inhibited DNA-induced DC survival. Moreover, while expression Bcl-xL depends...
Durable complete remission of metastatic renal cell carcinoma (RCC) has rarely been achieved with current treatment modalities. To solve this problem, alternative therapeutic options high efficacy and minimal side effects are strongly needed.Mesenchymal stem cells (MSC) were engineered to coexpress dodecameric TRAIL herpes simplex virus thymidine kinase (MSC/dTRAIL-TK). The antitumor MSCs expressing dTRAIL (MSC/dTRAIL) or HSV-TK alone (MSC/TK) MSC/dTRAIL-TK compared murine RCC using in vitro...
Here, we evaluated the safety and immunogenicity of hepatitis B virus (HBV) DNA vaccine, HB-110, in mice Korean patients with chronic (CHB) undergoing adefovir dipivoxil (ADV) treatment.For animal study, (BALB/c or HBV transgenic) were immunized mHB-110, T-cell antibody responses evaluated. For clinical 27 randomly received either ADV alone combination HB-110. Liver function tests, serum levels presence HBeAg/anti-HBe analysed. estimated by ELISPOT FACS analysis.mHB-110 induced higher than...
A low lymphocyte count puts immune-compromised patients at risk of mortality. hIL-7-hyFc is a homodimeric interleukin-7 (IL-7), potent T-cell amplifier, fused to the hybridizing IgD/IgG4 immunoglobulin domain. We performed randomized, double-blind, placebo-controlled, dose-escalation, phase I study assess pharmacokinetic, pharmacodynamic, safety, tolerability, and immunogenicity profiles administered s.c. i.m. healthy volunteers. Thirty subjects randomly received or its matching placebo in...
The unprecedented and rapid spread of SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2) has motivated the need for a rapidly producible scalable vaccine. Here, we developed synthetic soluble spike (S) DNA-based vaccine candidate, GX-19. In mice, immunization with GX-19 elicited not only S-specific systemic pulmonary antibody responses but also Th1-biased T cell in dose-dependent manner. GX-19-vaccinated nonhuman primates seroconverted exhibited detectable neutralizing response as...
Immune correlates of protection against hepatitis C virus (HCV) infection are not well understood. Here we investigated 2 naive and 6 immunized chimpanzees before after intravenous challenge, 12 weeks the last immunization, with 100 50% chimpanzee infectious doses (CID50) heterologous genotype 1b HCV. Vaccination recombinant DNA adenovirus vaccines expressing HCV core, E1E2, NS3-5 genes induced long-term HCV-specific antibody T-cell responses reduced peak viral load about times compared...
In this study, we have constructed various DNA vaccine vectors that carried hepatitis C virus (HCV) envelope genes without and with the granulocyte-macrophage colony-stimulating factor (GM-CSF) gene in several different ways. Buffalo rats received plasmids carrying HCV genes, which encode proteins E1 E2, both antibody lymphoproliferative responses against these were induced. These greatly enhanced by codelivery of GM-CSF gene. particular, inoculation a bicistronic plasmid independently...