A5100630683- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Retinal Diseases and Treatments
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Neuroscience and Neural Engineering
- Endoplasmic Reticulum Stress and Disease
- Alzheimer's disease research and treatments
- Clusterin in disease pathology
- Mitochondrial Function and Pathology
- Glaucoma and retinal disorders
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Retinoids in leukemia and cellular processes
- RNA regulation and disease
- Natural product bioactivities and synthesis
- Nuclear Receptors and Signaling
- Circadian rhythm and melatonin
- Connexins and lens biology
- Heat shock proteins research
- Zebrafish Biomedical Research Applications
- Potassium and Related Disorders
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Machine Learning in Bioinformatics
Stanford University
2020-2025
VA Palo Alto Health Care System
2021-2025
Pusan National University
2011-2024
University of Southern California
2012-2023
Palo Alto Institute
2023
Sookmyung Women's University
2022
Doheny Eye Institute
2008-2021
Smith-Kettlewell Eye Research Institute
2020
University of California, San Diego
2020
Southern California Eye Institute
2015-2019
This study aimed to investigate the genes and pathways that respond heat stress in Holstein bull calves exposed severe ranges of temperature humidity. A total ten animals from 4 6 months age were subjected at 37 °C 90 % humidity for 12 h. Skin rectal temperatures measured before after stress; while no correlation was found between them stress, a moderate detected confirming be better barometer monitoring stress. RNAseq analysis identified 8567 differentially regulated, out which 465...
Abstract Motivation: Identifying altered pathways in an individual is important for understanding disease mechanisms and the future application of custom therapeutic decisions. Existing pathway analysis techniques are mainly focused on discovering between normal cancer groups not suitable identifying aberrance that may occur sample. A simple way to identify individual’s compare tumor data from same individual. However, matched often unavailable clinical situation. Therefore, we suggest a new...
Pigs with SCID can be a useful model in regenerative medicine, xenotransplantation, and cancer cell transplantation studies. Utilizing genome editing technologies such as CRISPR/Cas9 system allows us to generate genetically engineered pigs at higher efficiency. In this study, we report generation phenotypic characterization of IL2RG knockout female produced through combination SCNT. As expected, lacking presented phenotype.First, specific systems targeting were introduced into developing pig...
Abstract Retinitis Pigmentosa (RP) is a blinding disease that arises from loss of rods and subsequently cones. The P23H rhodopsin knock-in (P23H-KI) mouse develops retinal degeneration mirrors RP phenotype in patients carrying the orthologous variant. Previously, we found protein was degraded P23H-KI retinas, Unfolded Protein Response (UPR) promoted degradation heterologous cells vitro. Here, investigated role UPR regulator gene, activating transcription factor 6 ( Atf6 ), homeostasis...
We have determined the impact of rod death and cone reorganization on spatiotemporal receptive fields (RFs) spontaneous activity distinct retinal ganglion cell (RGC) types. compared RGC function between healthy retinitis pigmentosa (RP) model rats (S334ter-3) at a time when nearly all rods were lost but cones remained. This allowed us to determine cone-mediated visual signaling, relevant point because diagnosis RP frequently occurs patients are nightblind daytime vision persists. Following...
The development of cholinergic cells in the rat retina has been examined with immunocytochemistry by using antisera against choline acetyltransferase (ChAT). ChAT-immunoreactive (IR) were first detected at embryonic day 17 (E17) transitional zone between neuroblastic layer (NBL) and ganglion cell (GCL). At E20, ChAT-IR are located exclusively GCL. postnatal 0 (P0), ChAT immunoreactivity appeared for time distal margin NBL. Two prominent bands labeled processes visible P3, P15, these two...
Retinitis Pigmentosa (RP) begins with the death of rod photoreceptors and is slowly followed by a gradual loss cones rearrangement remaining retinal neurons. Clusterin chaperone protein that protects cells involved in various pathophysiological stresses, including degeneration. Using well-established transgenic rat model RP (rhodopsin S334ter), we investigated effects clusterin on photoreceptor survival. To investigate role S334ter-line3 retinas, Voronoi analysis immunohistochemistry were...
Achromatopsia (ACHM) is an autosomal recessive disease that results in severe visual loss. Symptoms of ACHM include impaired acuity, nystagmus, and photoaversion starting from infancy; furthermore, associated with bilateral foveal hypoplasia absent or severely reduced cone photoreceptor function on electroretinography. Here, we performed genetic sequencing 3 patients 2 families ACHM, identifying functionally characterizing mutations the activating transcription factor 6 (ATF6) gene. We...
As part of an ongoing natural product chemical research for the discovery bioactive secondary metabolites with novel structures, wild fruiting bodies Daedaleopsis confragosa were collected and subjected to biological analyses. We fractions derived from methanol extract D. bioactivity-guided fractionation because showed antibacterial activity against Helicobacter pylori strain 51, according our bioactivity screening. The n-hexane dichloromethane moderate weak H. active analyzed isolation...
Activating transcription factor 6 (ATF6) is a key regulator of the unfolded protein response (UPR) and important for ER function homeostasis in metazoan cells. Patients carrying loss-of-function ATF6 disease alleles develop cone dysfunction disorder achromatopsia. The effect loss on other cell types, organs, diseases people remains unclear. Here, we report that progressive sensorineural hearing was notable complaint some patients Atf6-/- mice also showed auditory deficits affecting both...
Matrix metalloproteinases (MMPs) are involved in the pathology of numerous inflammatory retinal degenerations, including retinitis pigmentosa (RP). Our previous work revealed that intravitreal injections with tissue inhibitor 1 (TIMP-1) reduce progression rod cell death and inhibit cone remodeling involves reactive gliosis Müller glial cells (MGCs) rodent models. The underlying cellular molecular mechanisms how TIMP-1 functions retina remain to be resolved; however, MGCs structural...