A5074805682- Enzyme Structure and Function
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Diet, Metabolism, and Disease
- Pharmacogenetics and Drug Metabolism
- RNA modifications and cancer
- Protein purification and stability
- Connexins and lens biology
- RNA and protein synthesis mechanisms
- Microbial Natural Products and Biosynthesis
- Yersinia bacterium, plague, ectoparasites research
- Virus-based gene therapy research
- Chemical Reactions and Isotopes
- Innovative Microfluidic and Catalytic Techniques Innovation
- Epigenetics and DNA Methylation
- Erythrocyte Function and Pathophysiology
- Heat shock proteins research
- RNA Interference and Gene Delivery
- Bacterial Genetics and Biotechnology
- Chromium effects and bioremediation
- Transgenic Plants and Applications
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Monoclonal and Polyclonal Antibodies Research
- Polyamine Metabolism and Applications
- PI3K/AKT/mTOR signaling in cancer
Korea University
2020-2023
Seoul National University
1992-2020
Institute for Basic Science
2018-2020
Seoul Institute
2020
Government of the Republic of Korea
2020
Pohang University of Science and Technology
2007-2017
Ewha Womans University
2015-2016
Jeonbuk National University
2007
University of Wisconsin–Madison
1994
Abstract Connexin family proteins assemble into hexameric hemichannels in the cell membrane. The dock together between two adjacent membranes to form gap junction intercellular channels (GJIChs). We report cryo-electron microscopy structures of Cx43 GJICh, revealing dynamic equilibrium state various channel conformations detergents and lipid nanodiscs. identify three different N-terminal helix Cx43—gate-covering (GCN), pore-lining (PLN), flexible intermediate (FIN)—that are randomly...
Nanotechnology has been applied to the development of more effective and compatible drug delivery systems for therapeutic proteins. Human growth hormone (hGH) was fused with a hybrid Fc fragment containing partial domains human IgD IgG4 produce long-acting fusion protein. The protein, hGH-hyFc, resulted in increase hydrodynamic diameter (ca. 11 nm) compared 5 recombinant hGH. A diblock copolymer membrane nanopores (average 14.3 exhibited constant release rate hGH-hyFc. hGH-hyFc protein...
Direct-acting agents against viral components are considered as the most promising candidates for successful antiviral therapeutics. To date, no direct-acting drugs exist treatment dengue virus (DV) infection, which can develop into life-threatening diseases. RNA-dependent RNA polymerase (RdRp), an virus-specific enzyme highly conserved among various families, has been known broad-range drug target. Here, we developed RNA-based graphene biosensor system [RNA nano-graphene oxide (RANGO)] to...
YgjG is a putrescine aminotransferase enzyme that transfers amino groups from compounds with terminal primary amines to an aldehyde group using pyridoxal-5′-phosphate (PLP) as cofactor. Previous biochemical data show the prefers diamines, such putrescine, over ornithine substrate. To better understand enzyme's substrate specificity, crystal structures of Escherichia coli were determined at 2.3 and 2.1 Å resolutions for free putrescine-bound enzymes, respectively. Sequence structural analyses...
The DNA sequence that encodes 23S rRNA domain V of Bacillus subtilis, nucleotides 2036 to 2672 (C. J. Green, G. C. Stewart, M. A. Hollis, B. S. Vold, and K. F. Bott, Gene 37:261-266, 1985), was cloned used as a template from which transcribe defined RNA in vitro. transcripts served substrate vitro for specific methylation subtilis adenine 2085 (adenine 2058 Escherichia coli rRNA) by the ErmSF methyltransferase, an enzyme confers resistance macrolide-lincosamide-streptogramin B group...
The bromodomain and extraterminal domain (BET) family proteins recognize acetyl-lysine (K
KSI (ketosteroid isomerase) catalyses an allylic isomerization reaction at a diffusion-controlled rate. A hydrogen bond network, Asp99···Water504···Tyr14···Tyr55···Tyr30, connects two critical catalytic residues, Tyr14 and Asp99, with Tyr30, Tyr55 water molecule in the highly apolar active site of Pseudomonas putida KSI. In order to characterize interactions among these amino acids network KSI, double-mutant cycle analysis was performed, crystal structure each mutant protein within...
A fluorescent nanobiosensor for efficient quantitative analysis of the cancer-associated, m<sup>6</sup>A RNA demethylase ALKBH5 activity is developed, which shows high potential robust anticancer drug screening against demethylase.
The aim of this study was to identify apoptosis-related genes ovarian cancer cell lines following cisplatin treatment.We used IC(50) values and fluorescence-activated sorting analysis compare death in 2 lines, namely, SKOV-3 OVCAR-3, upon treatment with cisplatin. Moreover, the change transcriptional levels apoptosis-associated measured a dendron-modified DNA microarray.The protein for up-regulated each line were validated molecules that may determine cellular behavior resistance. Eight...
Proteins have evolved to compensate for detrimental mutations. However, compensatory mechanisms protein defects are not well understood. Using ketosteroid isomerase (KSI), we investigated how second-site mutations could recover defective mutant function and stability. Previous results revealed that the Y30F mutation rescued Y14F, Y55F Y14F/Y55F mutants by increasing catalytic activity 23-, 3- 1.3-fold, respectively, stability 3.3 kcal/mol. To better understand these observations,...
Affinity chromatography utilizing specific interactions between therapeutic proteins and bead-immobilized capturing agents is a standard method for protein purification, but its scalability limited by long purification times, activity loss the molecules and/or purified protein, high costs. Here, we report platform purifying antibodies via affinity precipitation using endogenous calcium ion-binding calsequestrin (CSQ), which undergoes ion-dependent phase transition. In this method, ZZ-CSQ...
Failure to detect the intermediate in spite of its existence often leads conclusion that two‐state transition unfolding process protein can be justified. In contrast previous equilibrium experiment fitted a model by circular dichroism and fluorescence spectroscopies, an dimeric ketosteroid isomerase (KSI) could detected small angle X‐ray scattering (SAXS) analytical ultracentrifugation. The sizes KSI were determined 18.7 Å 0 M urea, 17.3 5.2 25.1 7 urea SAXS. size was significantly decreased...
e14566 Background: Esophageal squamous cell carcinoma (ESCC) has been generally considered as one of the most aggressive cancers with poor prognosis. Vimentin regarded a marker epithelial-mesenchymal transition (EMT). However, little is known about ESCC vimentin expression EMT. In this study, we analyzed and its behavior. Methods: One hundred twenty-nine were surgically resected at Hirosaki University Hospital from 1996 to 2008. All patients underwent operations including subtotal...
MutL is required to assist the mismatch repair protein MutS during initiation of methyl-directed (MMR) response in various organisms ranging from prokaryotes eukaryotes. Despite this necessity, inherent propensity aggregate has led significant difficulties determining its biological relationship with other MMR-related proteins. Here, we perform analysis on thermostable found Thermotoga maritima MSB8 (TmL). Size exclusion chromatographic indicates lack aggregated forms exception a dimeric...
β-Lactam antibiotics that inhibit penicillin-binding proteins (PBPs) have been widely used in the treatment of bacterial infections. However, molecular basis underlying different inhibitory potencies β-lactams against specific PBPs is not fully understood. Here, we present crystal structures protein D2 (PBPD2) from Listeria monocytogenes, a Gram-positive foodborne pathogen causes listeriosis humans. The acylated complex with four (penicillin G, ampicillin, cefotaxime, and cefuroxime)...
The backbone dynamics of Y14F mutant Delta(5)-3-ketosteroid isomerase (KSI) from Comamonas testosteroni has been studied in free enzyme and its complex with a steroid analogue, 19-nortestosterone hemisuccinate (19-NTHS), by 15N NMR relaxation measurements. Model-free analysis the data showed that single-point mutation induced substantial decrease order parameters (S2) KSI, indicating structures KSI became significantly mobile mutation, while chemical shift indicated structural perturbations...