A5058668744- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Force Microscopy Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Tissue Engineering and Regenerative Medicine
- Advanced Sensor and Energy Harvesting Materials
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Microplastics and Plastic Pollution
- Cardiac electrophysiology and arrhythmias
- Cellular Mechanics and Interactions
- Advanced Biosensing Techniques and Applications
- Analytical Chemistry and Sensors
- Epigenetics and DNA Methylation
- Adhesion, Friction, and Surface Interactions
- Metabolomics and Mass Spectrometry Studies
- Gastroesophageal reflux and treatments
- Nanopore and Nanochannel Transport Studies
- Nanofabrication and Lithography Techniques
- Advanced Proteomics Techniques and Applications
- Bone and Dental Protein Studies
- Block Copolymer Self-Assembly
- RNA and protein synthesis mechanisms
Chonnam National University
2015-2024
Chonnam National University Hospital
2023
Pohang University of Science and Technology
2007-2017
Singapore Bioimaging Consortium
2015
Agency for Science, Technology and Research
2015
Star Technology and Research (United States)
2015
University of Washington
2014
Seattle University
2014
Korea Advanced Institute of Science and Technology
1999
A new drug delivery device using cylindrical block copolymer nanochannels was successfully developed for controlled protein applications. Depending on the hydrodynamic diameter of drugs, pore size in could be precisely down to 6 nm by Au deposition. Zero-order release bovine serum albumin (BSA) and human growth hormone (hGH) single-file diffusion, which has been observed gas diffusion through zeolite pores, realized up 2 months without denaturation. Furthermore, a nearly constant vivo hGH...
Abstract Supplementation of mesenchymal stem cells (MSCs) at sites bone resorption is required for homeostasis because the non-proliferation and short lifespan properties osteoblasts. Calcium ions (Ca 2+ ) are released from surfaces during osteoclast-mediated resorption. However, how elevated extracellular Ca concentrations would alter MSCs behavior in proximal largely unknown. In this study, we investigated effect on phenotype depending concentrations. We found that promoted cell...
Polystyrene nanoparticles (PS-NPs) derived from both environmental and occupational sources are an important class of ultrafine particles associated with human pulmonary disorders. The effects surface charges particle internalization toxicity to alveolar cells, especially under conditions comparable those found during breathing, have not been examined. Here, we applied cyclic stretches (CS) cells nanoparticle exposure show enhanced accumulation positively charged polystyrene as compared...
Sirtuins are NAD + ‐dependent deacetylases that regulate a range of cellular processes. Although diverse functions sirtuins have been proposed, those SIRT 6 and 7 mediated by their interacting proteins remain elusive. In the present study, we identified 6‐ 7‐interacting proteins, compared interactomes to investigate functional links. Our revealed 136 for 233 while confirming seven 111 previously 7, respectively. Comparison under same experimental conditions disclosed shared implying related...
BNIP3 (BCL2/adenovirus E1B 19 kDa interacting protein 3) is an atypical BH3-only that induced by hypoxia-inducible factor 1 (HIF1) under hypoxia. primarily regulated at the transcriptional level. However, little known about underlying mechanism of degradation. In this study, we found was downregulated when hypoxia accompanied amino acid starvation. The downregulation did not occur transcription level and independent HIF1A. degraded proteasome, but subjected to both proteasomal autophagic...
This paper describes the surface-patterned polydimethylsiloxane (PDMS) pillar arrays for enhancing cell alignment and contraction force in cardiomyocytes. The PDMS micropillar (μpillar) with microgrooves (μgrooves) were fabricated using a unique micro-mold made SU-8 double layer processes. spring constant of μpillar was experimentally confirmed atomic microscopy (AFM). After culturing cardiac cells on two different types arrays, without grooves top μpillar, characteristics cardiomyocytes...
Given their pervasiveness in the environment, particularly aquatic ecosystems, plastics are posing a growing concern worldwide. Many vertebrates and invertebrates marine, freshwater, terrestrial ecosystems exhibit microplastic (MP) uptake accumulation. Some studies have indicated fatal impacts of MPs on animals possible transfer through food chains. Thus, it is crucial to study MP pollution its environment-sensitive globally threatened animal groups, such as amphibians, which also play an...
Abstract Drug-induced cardiac toxicity is a critical concern in drug development, often leading to unreliable results and potential withdrawal due the lack of mature cardiomyocytes screening assays. This study aimed enhance cardiomyocyte maturation by combining structural conductive stimuli, specifically using Poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) coated PDMS membrane mechanical stimulation (MS) system. A 10% tensile strain at frequency 1 Hz was applied via...
Preclinical drug screening for cardiac toxicity has traditionally relied on observing changes in cardiomyocytes' electrical activity, primarily through invasive patch clamp techniques or non-invasive microelectrode arrays (MEA). However, relying solely field potential duration (FPD) measurements electrophysiological assessment can miss the full spectrum of drug-induced toxicity, as different drugs affect cardiomyocytes various mechanisms. A more comprehensive approach, combining and...
Abstract Cyclic stretch applied to cells induces the reorganization of stress fibers. However, correlation between fiber subtypes and strain-dependent responses cytoplasm nucleus has remained unclear. Here, we investigated dynamic involvement in orientation elongation cyclically stretched epithelial cells. We uniaxial cyclic stretches at 5%, 10%, 15% strains followed by release mechanical stretch. Dorsal, transverse arcs, peripheral fibers were mainly involved whereas perinuclear cap...
We propose a novel cantilever device integrated with polydimethylsiloxane (PDMS)-encapsulated crack sensor that directly measures the cardiac contractility. The was chemically bonded to PDMS thin layer form sandwiched structure which allows be operated very stably in culture media. reliability of proposed has improved dramatically compared no encapsulation layer. After evaluating durability layer, cardiomyocytes were cultured on nano-patterned for real-time measurement contractile forces....
Detection of adverse effects cardiac toxicity at an early stage by in vitro methods is crucial for the preclinical drug screening. Over years, several kinds biosensing platforms have been proposed scientific society detection toxicity. However, tissue optimized to measure either mechanophysiology or electrophysiology cardiomyocytes but not both. Herein, we demonstrate detail our successful attempt toward developing a novel "multifunctional microphysiological system" also known as...
Nanotechnology has been applied to the development of more effective and compatible drug delivery systems for therapeutic proteins. Human growth hormone (hGH) was fused with a hybrid Fc fragment containing partial domains human IgD IgG4 produce long-acting fusion protein. The protein, hGH-hyFc, resulted in increase hydrodynamic diameter (ca. 11 nm) compared 5 recombinant hGH. A diblock copolymer membrane nanopores (average 14.3 exhibited constant release rate hGH-hyFc. hGH-hyFc protein...
The importance of DNA sequencing in the life sciences and personalized medicine is continually increasing. Single-molecule methods have been developed to analyze directly without need for amplification. Here, we present a new approach single molecules using atomic force microscopy (AFM). In our approach, four surface-conjugated nucleotides were examined sequentially with polymerase-immobilized AFM tip. By observing specific rupture events upon examination matching nucleotide, could determine...