A5025137149- Alzheimer's disease research and treatments
- Amyotrophic Lateral Sclerosis Research
- Parkinson's Disease Mechanisms and Treatments
- Antimicrobial Peptides and Activities
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Advanced Fluorescence Microscopy Techniques
- Mitochondrial Function and Pathology
- Cholinesterase and Neurodegenerative Diseases
- Digital Holography and Microscopy
- Cellular transport and secretion
- Genetic Neurodegenerative Diseases
- Spectroscopy Techniques in Biomedical and Chemical Research
- Neuroscience and Neuropharmacology Research
- Glioma Diagnosis and Treatment
- Axon Guidance and Neuronal Signaling
- Biochemical and Structural Characterization
- Nanoplatforms for cancer theranostics
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Microtubule and mitosis dynamics
- Bacterial Identification and Susceptibility Testing
- Cellular Mechanics and Interactions
- Circular RNAs in diseases
- Neurogenetic and Muscular Disorders Research
Korea Basic Science Institute
2016-2025
Chung-Ang University
2023-2025
Korea University of Science and Technology
2015-2025
Hanyang University
2025
Seoul National University
2000-2022
Gwangju University
2021
Soonchunhyang University Hospital
2021
Dankook University
2020
Stanford University
2010-2018
Korea Research Institute of Bioscience and Biotechnology
2015-2018
Mutations in Pten-induced kinase 1 (PINK1) are linked to early-onset familial Parkinson's disease (FPD). PINK1 has previously been implicated mitochondrial fission/fusion dynamics, quality control, and electron transport chain function. However, it is not clear how these processes interconnected whether they sufficient explain all aspects of pathogenesis. Here we show that also controls motility. In Drosophila, downregulation dMiro or other components the machinery rescued dPINK1 mutant...
Calcium (Ca2+) homeostasis is essential for neuronal function and survival. Altered Ca2+ has been consistently observed in neurological diseases. How achieved various cellular compartments of disease-relevant cell types not well understood. Here we show Drosophila Parkinson's disease (PD) models that transport from the endoplasmic reticulum (ER) to mitochondria through ER-mitochondria contact site (ERMCS) critically regulates mitochondrial (mito-Ca2+) dopaminergic (DA) neurons, PD-associated...
Abstract The abnormal assembly of β‐amyloid (Aβ) peptides into neurotoxic, β‐sheet‐rich amyloid aggregates is a major pathological hallmark Alzheimer’s disease (AD). Light‐induced photosensitizing molecules can regulate Aβ amyloidogenesis. Multiple photochemical analyses using circular dichroism, atomic force microscopy, dot blot, and native gel electrophoresis verified that photoactivated meso ‐tetra(4‐sulfonatophenyl)porphyrin (TPPS with M=2H + , Zn 2+ Cu Mn ) successfully inhibits...
Polystyrene nanoparticles (PS-NPs) derived from both environmental and occupational sources are an important class of ultrafine particles associated with human pulmonary disorders. The effects surface charges particle internalization toxicity to alveolar cells, especially under conditions comparable those found during breathing, have not been examined. Here, we applied cyclic stretches (CS) cells nanoparticle exposure show enhanced accumulation positively charged polystyrene as compared...
Lipid droplet (LD) accumulation, a key feature of foam cells, constitutes an attractive target for therapeutic intervention in atherosclerosis. However, despite advances cellular imaging techniques, current noninvasive and quantitative methods have limited application living cells. Here, using optical diffraction tomography (ODT), we performed morphological biophysical analysis cells label-free manner. We identified LDs by verifying the specific refractive index correlative comprising ODT...
Mutations in leucine-rich repeat kinase 2 (LRRK2) are linked to familial as well sporadic forms of Parkinson's disease (PD), a neurodegenerative characterized by dysfunction and degeneration dopaminergic other types neurons. The molecular cellular mechanisms underlying LRRK2 action remain poorly defined. Here, we show that controls synaptic morphogenesis at the Drosophila neuromuscular junction. Loss results overgrowth, whereas overexpression LRRK or human has opposite effects. Alteration...
Despite the potential of photodynamic therapy (PDT), its comprehensive use in cancer treatment has not been achieved because nondegradable risks photosensitizing drugs and limits light penetration instrumentation. Here, we present bioluminescence (BL)-induced proteinaceous PDT (BLiP-PDT), through combination luciferase a reactive oxygen species (ROS)-generating protein (Luc-RGP), which is self-luminescent degradable. After exposure to coelenterazine-h as substrate for without external...
Abstract Background Cytoplasmic inclusions of transactive response DNA binding protein 43 kDa (TDP-43) in neurons and astrocytes are a feature some neurodegenerative diseases, such as frontotemporal lobar degeneration with TDP-43 (FTLD-TDP) amyotrophic lateral sclerosis (ALS). However, the role astrocyte pathology remains largely unknown. Methods To investigate whether overexpression primary could induce inflammation, we transfected plasmids encoding Gfp or TDP - . The inflammatory...
Abstract Glioblastoma multiforme (GBM) is the most fatal form of brain cancer in humans, with a dismal prognosis and median overall survival rate less than 15 months upon diagnosis. Glioma stem cells (GSCs), have recently been identified as key contributors both tumor initiation therapeutic resistance GBM. Both public dataset analysis direct differentiation experiments on GSCs demonstrated that CREB5 more highly expressed undifferentiated differentiated GSCs. Additionally, gene silencing by...
Abstract Antimicrobial peptides (AMPs) are promising agents for treating antibiotic‐resistant bacterial infections. Although discovering novel AMPs is crucial combating multidrug‐resistant bacteria and biofilm‐related infections, their clinical potential relies on precise, real‐time evaluation of efficacy, toxicity, mechanisms. Optical diffraction tomography (ODT), a label‐free imaging technology, enables visualization morphological changes, membrane damage, biofilm formation over time....
In both mammalian and insect models of ethanol-induced behavior, low doses ethanol stimulate locomotion. However, the mechanisms stimulant effects on CNS are mostly unknown. We have identified tao , encoding a serine-threonine kinase Ste20 family, as gene necessary for locomotor hyperactivity in Drosophila . Mutations also affect behavioral responses to cocaine nicotine, making flies resistant drugs. show that function is required during development adult nervous system mutations cause...
Abstract Alzheimer’s disease (AD) is a neurodegenerative characterized by genotypic and phenotypic heterogeneity. Critical components of the two AD pathological pathways, Aβ-amyloidosis Tauopathy, have been considered as therapeutic targets. Among them, much effort focused on aberrant Tau phosphorylation targeting Tau-phosphorylating kinases. Methylene blue (MB), phenothiazine dye that crosses blood-brain barrier, has shown to hit multiple molecular targets involved in beneficial effects...
Antimicrobial peptides (AMPs) are promising therapeutics in the fight against multidrug-resistant bacteria. As a mimic of AMPs, peptoids with N-substituted glycine backbone have been utilized for antimicrobials resistance proteolytic degradation. known to kill bacteria by membrane disruption; however, nonspecific aggregation intracellular contents is also suggested as an important bactericidal mechanism. Here,structure-activity relationship (SAR) library indole side chain-containing...
Axon extension and guidance require a coordinated assembly of F-actin microtubules as well regulated translation. The molecular basis how the translation mRNAs encoding proteins could be closely tied to pace cytoskeletal is poorly understood. Previous studies have shown that F-actin-microtubule crosslinker Short stop (Shot) required for motor sensory axon in Drosophila embryo. Here, we provide biochemical genetic evidence Shot functions with novel inhibitor, Krasavietz (Kra, Exba), steer...
TARDBP/TDP-43 (TAR DNA binding protein) proteinopathies are a common feature in variety of neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS), frontotemporal lobar degeneration (FTLD), and Alzheimer disease (AD). However, the molecular mechanisms underlying TARDBP-induced neurotoxicity largely unknown. In this study, we demonstrated that TARDBP induce impairment ubiquitin proteasome system (UPS), as evidenced by an accumulation ubiquitinated proteins reduction...
BMP2 is clinically used as an ectopic bone inducer and plays a significant role in development, formation, diseases. Chitinase 3-like 1 protein (Chi3L1) found the skeletal system. However, Chi3L1-mediated metabolism aging-related erosion via signaling have not yet been demonstrated. Herein, Chi3L1 increased BMP2-induced osteoblast differentiation mesenchymal precursor cells human primary osteoblasts. Chi3L1KO(-/-) showed abnormal osteoblasts isolated from exhibited impaired maturation. also...
Several lines of evidence suggest that endoplasmic reticulum (ER) stress plays a critical role in the pathogenesis many neurodegenerative diseases such as Alzheimer's disease, Parkinson's and amyotrophic lateral sclerosis.Protein tyrosine phosphatase 1B (PTP1B) is known to regulate ER signaling pathway, but its neuronal systems terms remains largely unknown.Here, we showed rotenone-induced toxicity human neuroblastoma cell mouse primary cortical neurons was ameliorated by PTP1B...