A5100339602- Cellular transport and secretion
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- COVID-19 Clinical Research Studies
- Parkinson's Disease Mechanisms and Treatments
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Bone fractures and treatments
- Mitochondrial Function and Pathology
- SARS-CoV-2 and COVID-19 Research
- Vector-borne infectious diseases
- Cancer Cells and Metastasis
- HIV/AIDS drug development and treatment
- Protein Structure and Dynamics
- Viral Infections and Vectors
- Microtubule and mitosis dynamics
- Calcium signaling and nucleotide metabolism
- Enzyme Structure and Function
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- Endoplasmic Reticulum Stress and Disease
- Lysosomal Storage Disorders Research
- Lipid Membrane Structure and Behavior
- Dermatological diseases and infestations
- Caveolin-1 and cellular processes
Beijing Institute of Neurosurgery
2025
Capital Medical University
2025
Université Paris-Saclay
2025
Inserm
2025
Institute of Animal Sciences
2025
Hubei Academy of Agricultural Sciences
2025
China Three Gorges University
2013-2025
Northwest A&F University
2025
Second Affiliated Hospital of Soochow University
2025
Soochow University
2024-2025
Mutations in Pten-induced kinase 1 (PINK1) are linked to early-onset familial Parkinson's disease (FPD). PINK1 has previously been implicated mitochondrial fission/fusion dynamics, quality control, and electron transport chain function. However, it is not clear how these processes interconnected whether they sufficient explain all aspects of pathogenesis. Here we show that also controls motility. In Drosophila, downregulation dMiro or other components the machinery rescued dPINK1 mutant...
Abstract Extracting knowledge‐based statistical potential from known structures of proteins is proved to be a simple, effective method obtain an approximate free‐energy function. However, the different compositions amino acid residues at core, surface, and binding interface prohibited establishment unified for folding despite fact that physical basis interaction (water‐mediated between acids) same. Recently, state ideal gas, rather than statistically averaged state, has been used as...
PINK1 loss-of-function mutations and exposure to mitochondrial toxins are causative for Parkinson's disease (PD) Parkinsonism, respectively. We demonstrate that pathological α-synuclein deposition, the hallmark pathology of idiopathic PD, induces dysfunction impairs mitophagy, driving accumulation substrate pS65-Ubiquitin (pUb) in primary neurons vivo. synthesized MTK458, a brain penetrant small molecule binds stabilizes an active heterocomplex, thereby increasing mitophagy. MTK458 mediates...
Metabolism dysfunction-associated fatty liver disease (MAFLD), is the most common chronic disease. Few MAFLD predictions are simple and accurate. We examined predictive performance of albumin-to-glutamyl transpeptidase ratio (AGTR), plasma atherogenicity index (AIP), serum uric acid to high-density lipoprotein cholesterol (UHR) for design practical, inexpensive, reliable models.
Mutations in PINK1 and Parkin cause familial, early onset Parkinson's disease. In Drosophila melanogaster, mutants show similar phenotypes, such as swollen dysfunctional mitochondria, muscle degeneration, energy depletion, dopaminergic (DA) neuron loss. We previously showed that genetically interact with the mitochondrial fusion/fission pathway, were recently proposed to form a quality control system involves mitophagy. However, vivo relationships among PINK1/Parkin function, fission/fusion,...
<title>Abstract</title> PINK1 loss-of-function mutations and exposure to mitochondrial toxins are causative for Parkinson’s disease (PD) Parkinsonism, respectively. We demonstrate that pathological α-synuclein deposition, the hallmark pathology of idiopathic PD, induces dysfunction, impairs mitophagy as evidenced by accumulation substrate pS65-Ubiquitin (pUb). discovered MTK458, a brain penetrant small molecule binds stabilizes its active complex, resulting in increased rates mitophagy....
Sec1 proteins are critical players in membrane trafficking, yet their precise role remains unknown. We have examined the of Sec1p regulation post-Golgi secretion Saccharomyces cerevisiae. Indirect immunofluorescence shows that endogenous is found primarily at bud neck newly budded cells and patches broadly distributed within plasma unbudded cells. Recombinant binds strongly to t-SNARE complex (Sso1p/Sec9c) as well fully assembled ternary SNARE (Sso1p/Sec9c;Snc2p), but also weakly free Sso1p....
Abstract Alzheimer’s disease (AD) is a neurodegenerative characterized by genotypic and phenotypic heterogeneity. Critical components of the two AD pathological pathways, Aβ-amyloidosis Tauopathy, have been considered as therapeutic targets. Among them, much effort focused on aberrant Tau phosphorylation targeting Tau-phosphorylating kinases. Methylene blue (MB), phenothiazine dye that crosses blood-brain barrier, has shown to hit multiple molecular targets involved in beneficial effects...
Abstract Background Rapid and simple serological assays for characterizing antibody responses are important in the current COVID-19 pandemic caused by SARS-CoV-2. Multiplex immunoblot (IB) termed IB were developed detecting IgG IgM antibodies to SARS-CoV-2 virus proteins patients. Methods Recombinant nucleocapsid protein S1, S2 receptor binding domain (RBD) of spike used as target antigens IBs. Specificity assay was established with 231 sera from persons allergy, unrelated viral infections,...
Munc18a is an SM protein required for SNARE-mediated fusion. The molecular details of how acts to enhance neurosecretion have remained elusive. Here, we use in vitro fusion assays characterize specific interactions between and the neuronal SNAREs rate extent We show that interacts directly functionally with preassembled t-SNARE complex. Analysis point mutations indicates helix C Syntaxin1a NRD Replacement SNAP25b yeast Sec9c had little effect, suggesting has minimal contact within A chimeric...
Eukaryotes employ elaborate mitochondrial quality control (MQC) to maintain the function of power-generating organelle. Parkinson's disease-associated PINK1 and Parkin actively participate in MQC. However, signaling events involved are largely unknown. Here we show that mechanistic target rapamycin 2 (mTORC2) Tricornered (Trc) kinases act downstream from regulate Trc is phosphorylated mTORC2-dependent mTORC2-independent manners specifically localized mitochondria response PINK1, which...
3-methyl-4-nitrophenol (PNMC), a chemical prevalent in various industries for drug, dye, and leather production, also serves as primary byproduct of organophosphate insecticides. Despite its global recognition an endocrine disruptor with documented reproductive toxicity, detrimental impact on preimplantation embryonic development has yet to be thoroughly investigated. In this study, through the vitro culture mice embryos, it was initially observed that even low concentrations PNMC exposure...
Zwitterionic energetic materials offer a unique combination of high performance and stability, yet their synthesis stability enhancement remain key challenges. In this study, we report the highly stable (dinitromethyl-functionalized zwitterionic compound, 1-(amino(iminio)methyl)-4,5-dihydro-1H-pyrazol-5-yl)dinitromethanide (4), with thermal decomposition temperature 215 °C, surpassing that most previously reported monocyclic zwitterions (Td < 150 °C). This compound was synthesized via...
BACKGROUND Ras-related protein Rab24, which belongs to the small GTPase family, plays a crucial role in regulating intracellular trafficking. Dysregulation of Rab24 has been recently identified hepatocellular carcinoma (HCC). However, its clinical significance and tumor related effects remain be further clarified. AIM To explore expression pattern HCC progression. METHODS The profile was tested tissues together with adjacent from transcriptional, mRNA, levels. prognostic assessed by...
SUMMARY Papain‐like cysteine proteases (PLCPs) are pivotal in plant development and immunity, though their specific regulatory mechanisms immune responses remain largely unexplored. In this study, we identify AtRD19C, a vacuole‐localized PLCP, demonstrate its role negatively regulating immunity to Phytophthora parasitica . We show that AtRD19C suppresses the ethylene (ET) signaling pathway by destabilizing copper chaperone AtATX1, which is essential for activating ET through receptor ETR1....
We present an efficient density-based adaptive-resolution clustering method APLoD for analyzing large-scale molecular dynamics (MD) trajectories. performs the k-nearest-neighbors search to estimate density of MD conformations in a local fashion, which can group same high-density region into cluster. greatly improves popular peaks algorithm by reducing running time and memory usage 2–3 orders magnitude systems ranging from alanine dipeptide 370-residue Maltose-binding protein. In addition, we...
The cell growth and ethanol yield of Zymomonas mobilis may be detrimentally affected by salt stress frequently present in some biomass-based fermentation systems, leading to a decrease the rate sugar conversion or other bioproducts. To address this problem, improving tolerance Z. is desirable way. However, limited progress has been made development with higher for technical challenges past decades. Recently, transposon insertion mutant system widely used as novel genetic tool many organisms...
Neuropeptide Y (NPY) exhibits a critical but poorly understood regulatory signaling function and has been shown to promote proliferation, vascularization migration in several types of cells tissues. However, little is known about the specific role NPY proliferation apoptosis bone marrow stromal (also as marrow-derived mesenchymal stem cells, BMSCs), which contain subpopulation multipotent skeletal cells. Based on BrdU incorporation tests, Cell Counting Kit-8, flow cytometry, quantitative...