A5058665881- CRISPR and Genetic Engineering
- Connexins and lens biology
- Virus-based gene therapy research
- Heat shock proteins research
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- Lipid Membrane Structure and Behavior
- Escherichia coli research studies
- Mass Spectrometry Techniques and Applications
- Cytomegalovirus and herpesvirus research
- Cancer therapeutics and mechanisms
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- nanoparticles nucleation surface interactions
- Yersinia bacterium, plague, ectoparasites research
- DNA and Nucleic Acid Chemistry
- Pancreatic function and diabetes
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Electron and X-Ray Spectroscopy Techniques
Korea University
2020-2023
Konkuk University
2017-2018
Cytosine and adenine base editor ribonucleoproteins show precise editing with reduced DNA RNA off-target effects.
Abstract Connexin family proteins assemble into hexameric hemichannels in the cell membrane. The dock together between two adjacent membranes to form gap junction intercellular channels (GJIChs). We report cryo-electron microscopy structures of Cx43 GJICh, revealing dynamic equilibrium state various channel conformations detergents and lipid nanodiscs. identify three different N-terminal helix Cx43—gate-covering (GCN), pore-lining (PLN), flexible intermediate (FIN)—that are randomly...
Connexin 36 (Cx36) is responsible for signal transmission in electrical synapses by forming interneuronal gap junctions. Despite the critical role of Cx36 normal brain function, molecular architecture junction channel (GJC) unknown. Here, we determine cryo-electron microscopy structures GJC at 2.2-3.6 Å resolutions, revealing a dynamic equilibrium between its closed and open states. In state, pores are obstructed lipids, while N-terminal helices (NTHs) excluded from pore. state with...
Abstract Connexin family proteins assemble into hexameric hemichannels in a cell membrane, which dock together between two adjacent membranes to form gap junction intercellular channels (GJIChs). The most ubiquitously expressed connexin Cx43 plays important roles numerous biological processes. Here we report cryo-EM structures of GJIChs at 3.1–3.6 Å resolutions, show dynamic conformational changes N-terminal helices (NTHs) caused by pH change or C-terminal truncation. channel-closing...
We report the crystal structure and bioinformatic analysis of SF173, a functionally uncharacterized protein from human enteropathogenic bacteria Shigella flexneri. The shows tightly interlinked dimer formed by adimeric core comprising α2 α3 helices both subunits swapping N-terminal α1 helix each monomer. Structural inspection genomic results suggest that SF173 might play its putative function binding to SF172, partially overlapped upstream product in operon. As YaeO (an SF172 orthologue) has...
DNA base editors and prime editing technology capable of therapeutic conversion enable ex vivo gene therapy for various genetic diseases. For such therapy, it is critical that the target cells survive well both outside body after transplantation. In this regard, chemically derived stem/progenitor are attracting attention as most useful cell sources clinical trials. Here, we generate hepatic progenitors from hereditary tyrosinemia type1 model mouse (HT1-mCdHs) successfully correct disease...
Summary DNA base editors and prime editing technology capable of therapeutic conversion enable ex vivo gene therapy for various genetic diseases. For such therapy, it is critical that the target cells survive well both outside body after transplantation. In this regard, chemically derived stem/progenitor are attracting attention as most useful cell sources clinical trials. Here, we generate hepatic progenitors from hereditary tyrosinemia type1 model mouse (HT1-mCdHs) successfully correct...
Growing demands for the structure determination of biological macromolecules using cryogenic electron microscopy (cryo-EM) necessitate expansion national research infrastructure.In particular, better accessibility to transmission microscopes (TEMs) is needed sample screening and high-resolution data collection as well newly emerging cryo-electron tomography (cryo-ET) analysis.Here we introduce recently established Cryo-EM Research Center at Pusan National University.The center equipped with...