A5081382387- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Advanced biosensing and bioanalysis techniques
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Virus-based gene therapy research
- Retinal Development and Disorders
- Periodontal Regeneration and Treatments
- Angiogenesis and VEGF in Cancer
- Cytomegalovirus and herpesvirus research
- Nanoplatforms for cancer theranostics
- Mosquito-borne diseases and control
- Innovation and Socioeconomic Development
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Graphene and Nanomaterials Applications
- Wound Healing and Treatments
- biodegradable polymer synthesis and properties
- Nanowire Synthesis and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Orthopaedic implants and arthroplasty
- Nanoparticles: synthesis and applications
Hanyang University
2018-2024
Seoul National University
2010-2024
Kangwon National University
2022-2024
Myongji University
2024
New Generation University College
2024
Korea Advanced Institute of Science and Technology
2024
Cell Biotech (South Korea)
2022
University of Seoul
2016-2017
Government of the Republic of Korea
2016-2017
Gold nanoparticles (AuNPs) have been extensively studied for photothermal cancer therapy because AuNPs can generate heat upon near-infrared irradiation. However, improving their tumor-targeting efficiency and optimizing the nanoparticle size maximizing effect remain challenging. We demonstrate that mesenchymal stem cells (MSCs) aggregate pH-sensitive gold (PSAuNPs) in mildly acidic endosomes, target tumors, be used therapy. These aggregated structures had a higher cellular retention...
Using stem cell-conditioned medium (CM) might be a viable alternative to cell transplantation, which is often hampered by low grafting efficiency and potential tumorigenesis, but the concentrations of angiogenic growth factors in CM are too for therapeutic use some components not human use. We used three-dimensional (3D) spheroid culture adipose-derived cells (ADSCs) with clinically relevant composed amino acids, vitamins, glucose, serum produce containing and/or antiapoptotic such as...
Cytosine and adenine base editor ribonucleoproteins show precise editing with reduced DNA RNA off-target effects.
Leber congenital amaurosis (LCA), an inherited retinal degeneration, causes severe visual dysfunction in children and adolescents. In patients with LCA, pathogenic variants, such as RPE65, are evident specific genes, related to the functions of pigment epithelium photoreceptors. contrast original Cas9, base editing tools can correct substitutions without generation DNA double-stranded breaks (DSBs). this study, dual adeno-associated virus (AAV) vectors containing split adenine editors (ABEs)...
Bone morphogenetic protein-2 (BMP-2) is known to induce both osteogenic and chondrogenic commitment of human mesenchymal stem cells (hMSCs). However, factors influencing BMP-2-dependent differentiation have not been investigated. In this study, we demonstrated that extracellular microenvironments, in the form cell-derived matrices, play important roles determining specific lineage hMSCs presence BMP-2. Extracellular matrices (ECMs) derived from osteoblasts chondrocytes were utilized regulate...
Implantation of ex vivo expanded and osteogenically differentiated mesenchymal stem cells (MSCs) for bone regeneration has drawbacks clinical applications, such as poor survival implanted increased treatment expenses. As a new approach that can circumvent these limitations, we propose dual delivery substance P (SP) morphogenetic protein-2 (BMP-2) to facilitate endogenous cell recruitment defects by SP subsequent in situ osteogenic differentiation those BMP-2. A heparin-conjugated fibrin...
Precise genome editing in human pluripotent stem cells (hPSCs) has potential applications isogenic disease modeling and ex vivo cell therapy, necessitating diverse tools. However, unlike differentiated somatic cells, hPSCs have unique cellular properties that maintain integrity, which largely determine the overall efficiency of an tool. Considering high demand for prime editors (PEs), it is imperative to characterize key molecular determinants PE outcomes hPSCs. Through homozygous knockout...
Abstract CRISPR-Cas9 nucleases are versatile tools for genetic engineering cells and function by producing targeted double-strand breaks (DSBs) in the DNA sequence. However, unintended production of large deletions (>100 bp) represents a challenge to effective application this genome-editing system. We optimized long-range amplicon sequencing system developed k-mer sequence-alignment algorithm simultaneously detect small alteration events deletions. With workflow, we determined that...
Cell therapy has been suggested as a treatment modality for ischemic diseases, but the poor survival and engraftment of implanted cells limit its therapeutic efficacy. To overcome such limitation, we used electrical stimulation (ES) derived from wearable solar cell inducing angiogenesis in tissue. ES enhanced secretion angiogenic growth factors migration mesenchymal stem (MSCs), myoblasts, endothelial progenitor cells, vitro. In mouse hindlimb model, generated by applied to region promoted...
For bone regeneration applications, scaffolds made from a composite of biodegradable polymer and ceramic have advantages over only one component (biodegradable or alone). In this study, simple rapid method was developed to induce hydroxyapatite (HA) nanoparticle adsorption on polyglycolic acid (PGA) scaffold surfaces. PGA meshes were coated with HA nanoparticles by immersing the in buffer solution containing 3,4-dihydroxyphenylalanine (DOPA), critical, functional element mussel adhesive...
ABA-triblock copolymers were prepared <italic>via</italic> ATRP, using modified perfluoropolyether (PFPE) as a macroinitiator (Br–PFPE–Br) to form the B block, and 2-hydroxy-3-cardanylpropyl methacrylate (HCPM) monomer A blocks.
Abstract Atherosclerosis development leads to irreversible cascades, highlighting the unmet need for improved methods of early diagnosis and prevention. Disturbed flow formation is one earliest atherogenic events, resulting in increased endothelial permeability subsequent monocyte recruitment. Here, a mesenchymal stem cell (MSC)‐derived nanovesicle (NV) that can target disturbed sites with peptide GSPREYTSYMPH (PREY) (PMSC‐NVs) presented which selected through phage display screening hundred...
Differentiation of stem cells can be controlled with interactions microenvironments the cells. The contain various signals including soluble growth factor signal, cell adhesion and mechanical which modulate differentiation Biomaterials provide these types to induce desirable cellular differentiation. deliver factors locally at a rate for long period. Stem specific molecules presented by biomaterials Mechanical delivered seeded onto biomaterial scaffolds. These approaches would invaluable...