A5100337288- Photoreceptor and optogenetics research
- Photosynthetic Processes and Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Hemoglobin structure and function
- Amino Acid Enzymes and Metabolism
- Retinal Development and Disorders
- Supramolecular Self-Assembly in Materials
- Photochromic and Fluorescence Chemistry
- Force Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Electron and X-Ray Spectroscopy Techniques
- Neuroscience and Neuropharmacology Research
- Surface and Thin Film Phenomena
- Quantum Dots Synthesis And Properties
- Mass Spectrometry Techniques and Applications
- Neuroscience and Music Perception
- Electrochemical sensors and biosensors
- Semiconductor materials and devices
- Hearing Loss and Rehabilitation
- Digital Holography and Microscopy
- Image Processing Techniques and Applications
- Music Technology and Sound Studies
- Copper Interconnects and Reliability
- Protein Structure and Dynamics
- Advanced X-ray Imaging Techniques
Sungkyunkwan University
2024-2025
Institute for Basic Science
2014-2023
Korea Advanced Institute of Science and Technology
2008-2021
Photoreceptor proteins play crucial roles in receiving light stimuli that give rise to the responses required for biological function. However, structural characterization of conformational transition photoreceptors has been elusive their native aqueous environment, even a prototype photoreceptor, photoactive yellow protein (PYP). We employ pump–probe X-ray solution scattering probe changes occur during photocycle PYP wide time range from 3.16 μs 300 ms. By analysis both kinetics and...
Proteins serve as molecular machines in performing their biological functions, but the detailed structural transitions are difficult to observe native aqueous environments real time. For example, despite extensive studies, solution-phase structures of intermediates along allosteric pathways for between relaxed (R) and tense (T) forms have been elusive. In this work, we employed picosecond X-ray solution scattering novel analysis track details dynamics wild-type homodimeric hemoglobin (HbI)...
Engineered defect chemistry in ultrathin (≈5 nm) hafnia through substitutional cobalt (HCO) is investigated for selective glucose sensing. Thin films of HCO, grown using chemical solution deposition (CSD)-traditionally used to grow thick films-on silicon, show significant sensing activity and undergo monoclinic orthorhombic phase transformation. The presence multivalent hafnia, with oxygen vacancies proximity, selectively oxidizes minimal interference from ascorbic acid, dopamine, uric acid....
Structural changes in global conformation and chromophore of photoactive yellow protein are probed real time by time-resolved X-ray solution scattering transient absorption spectroscopy.
Molecular switches alterable between two stable states by environmental stimuli, such as light and temperature, offer the potential for controlling biological functions. Here, we report a circular photoswitchable protein complex made of multiple molecules that can rapidly reversibly switch with significant conformational changes. The structural photochromic properties photoactive yellow (PYP) are harnessed to construct oligomer PYPs (coPYPs) desired sizes. Considering light-induced...
A novel optical holographic technique is presented to simultaneously measure both the real and imaginary components of complex refractive index (CRI) a protein solution over wide visible wavelength range. Quantitative phase imaging was employed precisely field transmitted from solution, which CRIs were retrieved using Fourier light scattering technique. Using this method, we characterized two dominant structural states photoactive yellow broad range (461-582 nm). The significant CRI...
This paper describes the design of a sound system used in human-robot interaction. Its objective is to play sounds synchronized with main (another application or robot) increase emotional gestural impact. synchronization possible using generic model composed states/transitions graph and different effects applied instantaneously. For each system, can be built. Every element also sound. enables dynamics system. The communicates this by sending messages network.
Photoactive yellow protein (PYP) induces negative phototaxis in Halorhodospira halophila via photoactivation triggered by light-mediated chromophore isomerization. Chromophore isomerization proceeds a volume-conserving mechanism due to the hydrogen-bond network and steric constraints inside protein, causes significant conformational changes accompanied N-terminal protrusion. However, it is unclear how structural change of affects remote domain. To understand photocycle-related changes, we...
Here, we introduce the nanoparticle-aided cryo-electron microscopy sampling (NACS) method to access conformational distribution of a protein molecule. Two nanogold particles are labeled at two target sites, and interparticle distance is measured as structural parameter via (cryo-EM). The key aspect NACS that projected information instead global extracted from each This possible because contrast provided by strong enough provide distance, while itself invisible due its low contrast. We...
Proteins have the potential to serve as nanomachines with well-controlled structural movements, and artificial control of their conformational changes is highly desirable for successful applications exploiting dynamic characteristics. Here, we demonstrate an experimental approach regulating degree change in proteins by incorporating a small-molecule linker into well-known photosensitive protein, photoactive yellow protein (PYP), which sensitized blue light undergoes photo-induced N-terminal...
A novel optical technique for measuring the complex refractive index (CRI) of photoactive proteins over wide range visible wavelengths is presented. Employing quantitative phase microscopy equipped with a wavelength swept source, fields transmitted from solution were precisely measured, which CRIs retrieved Fourier light scattering technique. Using present method, both real and imaginary RIs yellow protein (PYP) measured broad (461 - 582 nm). The internal population ground excited states...
Molecular switches alterable between two stable states by environmental stimuli such as light and temperature offer the potential for controlling biological functions. Here, we report a photoswitchable protein complex made of multiple molecules that can rapidly reversibly switch with significant conformational changes. The structural photochromic properties photoactive yellow (PYP) are harnessed to construct circular oligomer PYPs (coPYPs) desired sizes. Considering light-induced N-terminal...
Photoactive yellow protein (PYP) is one of the most extensively studied photoreceptors. Nevertheless, role N-terminus in photocycle and structural transitions still elusive. Here, we attached additional amino acids to PYP investigated effect length charge N-terminal residues using circular dichroism, two-dimensional nuclear magnetic resonance (2D-NMR), transient absorption (TA), grating (TG) spectroscopic techniques. TA experiments showed that, except for negatively charged (5D-PYP),...