A5035115503- Ubiquitin and proteasome pathways
- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Protein Degradation and Inhibitors
- PI3K/AKT/mTOR signaling in cancer
- Autophagy in Disease and Therapy
- Cancer Mechanisms and Therapy
- Nuclear Receptors and Signaling
- NF-κB Signaling Pathways
- Cell death mechanisms and regulation
- Parkinson's Disease Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Lipid metabolism and biosynthesis
- MicroRNA in disease regulation
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- Hippo pathway signaling and YAP/TAZ
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer-related gene regulation
- Polyamine Metabolism and Applications
- Metabolism, Diabetes, and Cancer
- Pharmacological Receptor Mechanisms and Effects
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
Korea Research Institute of Bioscience and Biotechnology
2014-2023
Korea University of Science and Technology
2014-2023
LG (South Korea)
2020-2023
LG Chem (South Korea)
2021-2022
Howard Hughes Medical Institute
2010-2015
University of Colorado Boulder
2015
University of Science and Technology
2013-2014
Universidad Mayor
2013
Korea Advanced Institute of Science and Technology
2000-2008
Creative Research
2006
Parkin, an E3 ubiquitin ligase, has been found to be responsible for autosomal recessive juvenile parkinsonism characterized primarily by selective loss of dopaminergic neurons with subsequent defects in movements. However, the molecular mechanisms underlying this neuron remain elusive. Here, we Drosophila parkin loss-of-function mutants, which exhibit shrinkage decreased tyrosine hydroxylase level and impaired locomotion. The behavioral defect mutant flies was partially restored...
G-protein coupled receptor 43 (GPR43) serves as a for short-chain fatty acids (SCFAs), implicated in neutrophil migration and inflammatory cytokine production. However, the intracellular signaling pathway mediating GPR43 remains unclear. Here, we show that β-arrestin 2 mediates internalization of by agonist. Agonism reduced phosphorylation nuclear translocation factor-κB (NF-κB), which was relieved short interfering RNA (siRNA) 2. Subsequently, mRNA expression proinflammatory cytokines,...
We have studied a possible role of extracellular zinc ion in the activation p70S6k, which plays an important progression cells from G(1) to S phase cell cycle. Treatment Swiss 3T3 with sulfate led and phosphorylation p70S6k dose-dependent manner. The by treatment was biphasic, early being at 30 min followed late 120 min. zinc-induced partially inhibited down-regulation phorbol 12-myristate 13-acetate-responsive protein kinase C (PKC) chronic 13-acetate, but this not significant. Moreover,...
Phosphoinositide-dependent kinase-1 (PDK-1) is a central mediator of the cell signaling between phosphoinositide 3-kinase (PI3K) and various intracellular serine/threonine kinases including Akt/protein kinase B (PKB), p70 S6 kinases, protein C. Recent studies with transfection experiments have implied that PDK-1 may be involved in functions growth apoptosis. However, despite its pivotal role cellular signalings, vivo multicellular system rarely been investigated. Here, we isolated Drosophila...
Triacylglycerol (TG) is the major form of stored energy in eukaryotic organisms and synthesized by diacylglycerol acyltransferase (DGAT) endoplasmic reticulum (ER). DGAT2, one two DGAT enzymes, barely detectable cells, even though its mRNA transcripts are maintained at considerable levels. However, little known about how DGAT2 expression altered protein stability. was highly unstable cells rapidly degraded proteasomes an ubiquitin-dependent manner. Deletion mutation analysis identified...
Abstract Proteolysis targeting chimeras (PROTACs) are an emerging strategy for promoting targeted protein degradation by inducing the proximity between proteins and E3 ubiquitin ligases. Although successful of numerous PROTACs has been demonstrated, elements that determine degradability PROTAC-targeted have not yet explored. In this study, we developed von Hippel-Lindau-Cereblon (VHL-CRBN) heterodimerizing induce CRBN, but VHL. A quantitative proteomic analysis further revealed VHL-CRBN...
The FOXO family of forkhead transcription factors has a variety important functions in stress response, metabolism, cell cycle, apoptosis, longevity, etc. transcriptional activity and subcellular localization are tightly regulated by post-translational modifications, including phosphorylation various kinases. Here, we report that the transforming growth factor-beta-activated kinase (TAK1)-Nemo-like (NLK) pathway negatively regulates FOXO1. We show NLK binds phosphorylates FOXO1 at...
Diacylglycerol acyltransferase 2 (DGAT2) is one of two distinct DGAT enzymes that catalyze the last step in triacylglycerol (TG) synthesis. Findings from previous studies suggest inhibition DGAT2 a promising strategy for treatment hepatic steatosis and insulin resistance. Here, we identified compound 122 as potent selective inhibitor human DGAT2, which appeared to act competitively against oleoyl-CoA vitro. The was also confirmed by reductions enzymatic activity de novo TG synthesis...
Akt is stimulated by several growth factors and has a major anti-apoptotic role in the cell. Therefore, we hypothesized that pathway leading to inhibition of might be utilized process apoptosis. Accordingly, used yeast two-hybrid screening assay identify proteins interact with possibly inhibit Akt. We found C-terminal region protein kinase C-related 2 (PRK2), containing amino acids 862 908, specifically binds mammalian cells. During early stages apoptosis, PRK2 cleaved from inhibitory...
Mitogen-activated protein kinase (MAPK) phosphatase 3 (MKP-3) is a well-known negative regulator in the Ras/extracellular signal-regulated (ERK)-MAPK signaling pathway responsible for cell fate determination and proliferation during development.However, physiological roles of MKP-3 mechanism by which regulates Ras/Drosophila ERK (DERK) vivo have not been determined.Here, we demonstrated that Drosophila (DMKP-3) critically involved differentiation, proliferation, gene expression suppressing...
Bi-Oh Park, Jong Soon Kang, Suresh Paudel, Sung Goo Byoung Chul Sang-Bae Han, Young-Shin Kwak, Jeong-Hoon Kim and Sunhong Kim. Biomolecules & Therapeutics 2022;30:48-54. https://doi.org/10.4062/biomolther.2021.078
Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5/GPR49) is highly expressed in adult stem cells of various tissues, such as intestine, hair follicles, and stomach. LGR5 also overexpressed some colon ovarian tumors. Recent reports show that R-spondin (RSPO) family ligands bind to activate LGR5, enhancing canonical Wnt signaling via the interaction with LRP5/6 Frizzled. The identity heterotrimeric G-proteins however, remains unclear. Here, we Rho GTPase a downstream target...