A5028429512- Mesenchymal stem cell research
- Bone Metabolism and Diseases
- Bone Tissue Engineering Materials
- Metabolism, Diabetes, and Cancer
- Periodontal Regeneration and Treatments
- Bone fractures and treatments
- Bone health and treatments
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Bone health and osteoporosis research
- Hedgehog Signaling Pathway Studies
- Oral and Maxillofacial Pathology
- dental development and anomalies
- Tissue Engineering and Regenerative Medicine
- Muscle Physiology and Disorders
- Cancer Cells and Metastasis
- Dental Trauma and Treatments
- Bone and Dental Protein Studies
- Pancreatic function and diabetes
- Ocular Disorders and Treatments
- Wnt/β-catenin signaling in development and cancer
- Laser Applications in Dentistry and Medicine
- Osteoarthritis Treatment and Mechanisms
Universidade de São Paulo
2020-2024
Aim: We evaluated the bone repair induced by MSCs from adipose tissue (AT-MSCs) and marrow (BM-MSCs) injected into rat calvarial defects at two time points. Methods & results: Both cell populations expressed MSC surface markers differentiated adipocytes osteoblasts. μCT showed that combination of cells distinct sources exhibited synergistic effects to increase with an advantage when BM-MSCs were prior AT-MSCs. The higher osteogenic potential these combinations was demonstrated using in vitro...
Aim: This study aimed to evaluate the ability of human periodontal ligament stem cells (PDLSCs) with high (HP-PDLSCs) and low (LP-PDLSCs) osteogenic potential, in addition mixed cells, repair bone tissue. Methods: Cell phenotype, proliferation differentiation were evaluated. Undifferentiated PDLSCs injected into rat calvarial defects new was evaluated by μCT, histology real-time PCR. Results: exhibited a typical mesenchymal cell phenotype HP-PDLSCs showed lower proliferative higher potential...
Background: The events of bone formation and osteoblast/titanium (Ti) interactions may be affected by Hedgehog Notch signalling pathways. Herein, we investigated the effects modulation these pathways on osteoblast differentiation caused nanostructured Ti (Ti-Nano) generated H2SO4/H2O2. Methods: Osteoblasts from newborn rat calvariae were cultured Ti-Control Ti-Nano in presence agonist purmorphamine or antagonist cyclopamine N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester...
Abstract Objective Hypertension disrupts the bone integrity and its repair ability. This study explores efficiency of a therapy based on application mesenchymal stem cells (MSCs) to defects spontaneously hypertensive rats (SHR). Methods First, we evaluated SHR in terms morphometry differentiation MSCs into osteoblasts. Then, effects interactions between from normotensive (NTR‐MSCs) cocultured with (SHR‐MSCs) osteoblast both cell populations were evaluated. Also, formation calvarial treated...
Abstract This study aimed to investigate if wingless‐related integration site (Wnt) signaling participates in the high osteogenic potential of titanium with nanotopography (Ti‐Nano). We showed that among several components Wnt pathway, Frizzled 6 ( Fzd6 ) was transcript most intensely modulated by compared untreated Ti surface (Ti‐Machined). Then, we investigated whether and how regulation osteoblast differentiation caused nanotopography. The silencing CRISPR–Cas9 transfection MC3T3‐E1 cells...
Tissue engineering and cell therapy have been the focus of investigations on how to treat challenging bone defects. This study aimed produce characterize a P(VDF-TrFE)/BaTiO3 scaffold evaluate effect mesenchymal stem cells (MSCs) combined with this photobiomodulation (PBM) repair.P(VDF-TrFE)/BaTiO3 was synthesized using an electrospinning technique presented physical chemical properties suitable for tissue engineering. implanted in rat calvarial defects (unilateral, 5 mm diameter) and, 2...
Therapies to prevent osteoporosis are relevant since it is one of the most common non-communicable human diseases in world and prevalent bone disorder adults. Since jaboticaba peel extract (JPE) added culture medium enhanced osteogenic potential mesenchymal stem cells (MSCs) derived from osteoporotic rats, we hypothesized that JPE prevents development ovariectomy-induced osteoporosis. Ovariectomized rats were treated with either (30 mg/kg body weight) or its vehicle for 90 days, starting 7...
Abstract Type 1 (T1DM) and type 2 (T2DM) diabetes mellitus are characterized by changes in glucose metabolism cause bone damage via a variety of mechanisms, including effects on osteoblasts. We aimed to evaluate the osteoblast differentiation mesenchymal stem cells (MSCs) from rats with T1DM or T2DM removing hyperglycemic stimulus osteogenic potential these cells. MSCs healthy were cultured normoglycemic medium, whereas medium. reduced grown media, having more pronounced effect, as evidenced...
Bone formation is driven by many signaling molecules including bone morphogenetic protein 9 (BMP-9) and hypoxia-inducible factor 1-alpha (HIF-1α). We demonstrated that cell therapy using mesenchymal stem cells (MSCs) overexpressing BMP-9 (MSCs+BMP-9) enhances in calvarial defects. Here, the effect of hypoxia on BMP components targets MSCs+BMP-9 these hypoxia-primed osteoblast differentiation repair was evaluated. Hypoxia induced with cobalt chloride (CoCl2) MSCs+BMP-9, expression The...
<title>Abstract</title> BACKGROUND Diabetes mellitus (DM) negatively impacts bone tissue, leading to loss and increased fracture risk with many in need of additional treatments, therapy based on mesenchymal stem cells (MSCs) represents a promising treatment for defects patients diabetes. The present investigation explored the interactions between MSCs from normoglycemic (NG-MSCs) diabetic (DM-MSCs) donors osteoblast differentiation effects NG-MSCs regeneration created rats. METHODS After DM...
Type 1 (T1DM) and type 2 (T2DM) diabetes mellitus are characterized by changes in glucose metabolism cause bone damage via a variety of mechanisms, including effects on osteoblasts. We aimed to evaluate the osteoblast differentiation mesenchymal stem cells (MSCs) from rats with T1DM or T2DM removing hyperglycemic stimulus osteogenic potential these cells. Morphometric parameters revealed that caused loss, having more deleterious effects. MSCs healthy were cultured normoglycemic medium,...
Aims: Type 1 (T1DM) and type 2 (T2DM) diabetes mellitus are characterized by changes in glucose metabolism both cause damages to bone through several mechanisms, including effects on osteoblasts. We aimed evaluate the osteoblast differentiation of mesenchymal stem cells (MSCs) from rats with T1DM or T2DM hyperglycemic stimulus deletion osteogenic potential these cells.Main methods key findings: Morphometric parameters showed that induced loss, more deleterious T2DM. MSCs healthy were grown...