Abstract Treatment of large bone defects is a challenging clinical situation that may be benefited from cell therapies based on regenerative medicine. This study was conducted to evaluate the effect local injection marrow-derived mesenchymal stromal cells (BM-MSCs) or adipose tissue-derived MSCs (AT-MSCs) regeneration rat calvarial defects. BM-MSCs and AT-MSCs were characterized their expression specific surface markers; viability evaluated after with 21-G needle. Defects measuring 5 mm...

Periodontal pathogenesis takes into consideration that disease results from a complex inflammatory immune response. Among the major cytokines related to periodontal damage, interleukin (IL)-6 enhances cascade of tissue destruction. Tocilizumab (TCZ) is humanized monoclonal anti-human IL-6 receptor inhibits IL-6-mediated proinflammatory activity. This study aimed elucidate whether TCZ deleterious effect ligature-induced periodontitis.Experimental periodontitis was treated with systemic...

The ability of hemostatic agents to promote bone repair has been investigated using in vitro and vivo models but, up now, the results are inconclusive. Objective In this context, aim study was compare potential collagen sponge with fibrin glue a rat calvarial defect model. Material Methods Defects 5 mm diameter were created calvariae treated either or glue; untreated defects used as control. At 4 8 weeks, histological analysis micro-CT-based histomorphometry carried out data compared by...

The poly(vinylidene-trifluoroethylene)/barium titanate (PVDF) membrane enhances in vitro osteoblast differentiation and vivo bone repair. Here, we hypothesized that this higher repair could be also due to resorption inhibition mediated by a microRNA (miR)/RANKL circuit. To test our hypothesis, the large-scale miR expression of tissue grown on PVDF polytetrafluoroethylene (PTFE) membranes was evaluated identify potential RANKL-targeted miRs modulated PVDF. animal model used rat calvarial...

Abstract Objective Hypertension disrupts the bone integrity and its repair ability. This study explores efficiency of a therapy based on application mesenchymal stem cells (MSCs) to defects spontaneously hypertensive rats (SHR). Methods First, we evaluated SHR in terms morphometry differentiation MSCs into osteoblasts. Then, effects interactions between from normotensive (NTR‐MSCs) cocultured with (SHR‐MSCs) osteoblast both cell populations were evaluated. Also, formation calvarial treated...

Aim: We tested the hypothesis that association of bone marrow mesenchymal stem cells (MSCs) and osteoblasts (OBs) optimize repair. Materials & Methods: MSCs were cultured in growth or osteogenic medium seeded into gelatin sponge prior to implantation. Defects created rat calvariae implanted with without cells, MSCs, OBs OBs. Histological analysis micro-CT-based histomorphometry carried out after 4 weeks. Results: Increased formation was observed defects treated volume greater either...

Aim: The aim of this study was to investigate the effect local injection osteoblasts differentiated from bone marrow (BM-OB) or adipose tissue (AT-OB) mesenchymal stromal cells on formation. Materials & methods: Defects were created in rat calvaria and injected with BM-OB AT-OB phosphate-buffered saline without as control. Bone formation evaluated 4 weeks postinjection. Results: Injection resulted higher than that obtained induced by cell injections exhibited similar mechanical properties...

Abstract The secretome present in the conditioned medium (CM) of mesenchymal stem cells (MSCs) is a promising tool to be used therapies promote bone regeneration. Considering high osteogenic potential morphogenetic protein 9 (BMP‐9), we hypothesized that MSCs overexpressing BMP‐9 (MSCs ) enhances osteoblast differentiation and formation calvarial defects. CM either (CM‐MSCs or without overexpression VPR were obtained at different periods. As CM‐MSCs generated after 1 h presented highest...

The aim of this study was to evaluate the buccal bone plate remodeling after immediate implantation using flapless approach with or without graft into gap between implant and bone.Eight dogs had mandibular bicuspids extracted flaps, four implants were installed on each side, totaling eight per animal. Randomly, in one positioned at crest level (equicrestal), opposite 2 mm subcrestal. All 2.0 from (gap) associated not grafting material. Therefore, following treatments performed: subcrestal...

Abstract Purpose The coating of implant surfaces with components the extracellular matrix offers an approach to influence peri‐implant bone healing. In this study, healing around coated implants is analyzed in a defect model. Materials and Methods Eight months after extraction premolar teeth, six dogs received 48 (eight per animal) mandible. Implant were sandblasted acid‐etched, some additionally collagen type II chondroitin sulfate (collagen/ CS ). On each side mandible, either had no...

Abstract Objectives This study evaluated buccal bone crest remodeling, socket composition after healing, and dimensional ridge preservation flapless tooth extraction procedures with or without a xenograft comparing histomorphometric microcomputed tomographic (micro‐ CT ) data. Material Methods The mandibular premolars of eight dogs were extracted flaps. One on each side received grafting material (test group), the other remained only blood clot (control group). Twelve weeks treatment, crest,...

Background: The purpose of this study was to evaluate the influence root trunk concavities on guided tissue regeneration. Methods: second, third, and fourth mandibular bicuspids 4 healthy mongrel dogs were used. Full flaps elevated furcation lesions (5 mm in height 2 depth) surgically created. One deep prepared trunks part crown all teeth. second remained as controls (C); left third received normal expanded polytetrafluoroethylene (ePTFE) membranes (NM) right modified ePTFE (MM). MM made by...

Abstract Objective Several implant surfaces are being developed, some in the nanoscale level. In this study, two different had their early healing properties compared context of circumferential defects various widths. Material and methods Six dogs mandibular premolars extracted. After 8 weeks, four implants were placed equicrestally each side. One acted as control, while others inserted into sites with 1.0, 1.5 2.0 mm wide 5 deep. A nano‐modified surface was used on one side a micro‐rough...

Abstract Aim To evaluate the influence of gingival thickness and bone grafting on buccal plate remodelling after immediate implant placement in sockets with thin bone, using a flapless approach. Materials Methods The gingiva eight dogs was thinned at one side mandible, mandibular premolars were extracted without flaps, four implants installed each 1.5 mm from bone. sites randomly assigned into: TG (test group) = gingiva; + GM ( material); CG (control normal material). After 12 weeks...

Therapies to prevent osteoporosis are relevant since it is one of the most common non-communicable human diseases in world and prevalent bone disorder adults. Since jaboticaba peel extract (JPE) added culture medium enhanced osteogenic potential mesenchymal stem cells (MSCs) derived from osteoporotic rats, we hypothesized that JPE prevents development ovariectomy-induced osteoporosis. Ovariectomized rats were treated with either (30 mg/kg body weight) or its vehicle for 90 days, starting 7...

Abstract Background Finding the most effective method of minimizing gap effect in alveolar crest remodeling constitutes a clinical challenge for immediate implant technique. Purpose To evaluate effectiveness osseoconductive xenografts with different porosities crestal bone region, and without guided regeneration, over installation. Materials Methods Five defects (6 mm diameter/4 depth) were prepared on one side mandibles twelve dogs. Implants 3.3 × 10 installed mesial each defect, providing...

Cell therapy associated with guided bone regeneration (GBR) can be used to treat defects under challenging conditions such as osteoporosis. This study aimed evaluate the effect of mesenchymal stem cells (MSCs) in combination a poly(vinylidene-trifluoroethylene)/barium titanate (PVDF-TrFE/BT) membrane on repair osteoporotic rats. Osteoporosis was induced female rats by bilateral removal ovaries (OVX) or sham surgery (SHAM), and condition characterized after 5 months microtomographic...

Aim The aim of this study was to evaluate the buccal bone remodeling after immediate implantation with flap or flapless approach. Material and Methods mandibular bilateral premolars 3 dogs were extracted immediately three implants placed in both hemi-arches each dog. Randomly, one hemi-arch treated approach, while contra lateral tooth extractions implant placement done mucoperiosteal elevation. Non-submerged healing 12 weeks provided for groups. Histomorphometric analysis compare lingual...

Bone formation is driven by many signaling molecules including bone morphogenetic protein 9 (BMP-9) and hypoxia-inducible factor 1-alpha (HIF-1α). We demonstrated that cell therapy using mesenchymal stem cells (MSCs) overexpressing BMP-9 (MSCs+BMP-9) enhances in calvarial defects. Here, the effect of hypoxia on BMP components targets MSCs+BMP-9 these hypoxia-primed osteoblast differentiation repair was evaluated. Hypoxia induced with cobalt chloride (CoCl2) MSCs+BMP-9, expression The...