A5029739354- Bone Tissue Engineering Materials
- Dental Implant Techniques and Outcomes
- Bone and Dental Protein Studies
- Periodontal Regeneration and Treatments
- Dental materials and restorations
- Orthopaedic implants and arthroplasty
- dental development and anomalies
- Bone Metabolism and Diseases
- Endodontics and Root Canal Treatments
- Oral and Maxillofacial Pathology
- Electrospun Nanofibers in Biomedical Applications
- Bone health and treatments
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Salivary Gland Tumors Diagnosis and Treatment
- Graphene and Nanomaterials Applications
- Facial Trauma and Fracture Management
- 3D Printing in Biomedical Research
- Orthodontics and Dentofacial Orthopedics
- Connective tissue disorders research
- Alkaline Phosphatase Research Studies
- Silk-based biomaterials and applications
- Cleft Lip and Palate Research
- Dental Radiography and Imaging
- Dental Trauma and Treatments
Universidade de São Paulo
2016-2025
University of Coimbra
2025
Universidade de Ribeirão Preto
2011-2021
Hospital Universitário da Universidade de São Paulo
2011
Centro de Neurociências e Biologia Celular
2010
Université de Montréal
2003-2009
Research Institute of Human Morphology
2009
Université du Québec à Montréal
2009
Plasmionique (Canada)
2009
Institut National de la Recherche Scientifique
2009
The surface characteristics of biomaterials can influence protein adsorption, cellular functions, and ultimately tissue formation. Controlled chemical oxidation titanium-based surfaces with a mixture H(2)SO(4)/H(2)O(2) creates nanopatterned that has been shown to affect early osteogenic events. objective this study was evaluate the effect over time nanopattern on various key parameters osteogenesis, determine whether these effects translate into more mineralized matrix production. Osteogenic...
A nanocomposite based on bacterial cellulose (BC) and type I collagen (COL) was evaluated for in vitro bone regeneration. BC membranes were modified by glycine esterification followed cross-linking of employing 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. Collagen incorporation studied spectroscopy analysis. X-Ray diffraction showed changes the crystallinity after incorporation. The elastic modulus tensile strength BC-COL decreased, while strain at failure a slight increase, even...
In the field of regenerative medicine, nanoscale physical cuing is clearly becoming a compelling determinant cell behavior. Developing effective methods for making nanostructured surfaces with well-defined physicochemical properties thus mandatory rational design functional biomaterials. Here, we demonstrate versatility simple chemical oxidative patterning to create unique nanotopographical that influence behavior various types, modulate expression key determinants activity, and offer...
In the course of attempting to define bone "secretome" using a signal-trap screening approach, we identified gene encoding small membrane protein novel osteoblasts. Although previously in silico as ifitm5, no localization or functional studies had been undertaken on this gene. We characterized expression patterns and vitro vivo assessed its role matrix mineralization vitro. The specificity shown led us rename restricted ifitm-like (Bril). Bril encodes 14.8-kDa 134 amino acid with two...
A common subject in bone tissue engineering is the need for porous scaffolds to support cell and interactions aiming at repairing tissue. As poly(lactide-co-glycolide)–calcium phosphate (PLGA–CaP) can be manufactured with different pore sizes, aim of this study was evaluate effect diameter on osteoblastic responses formation. Scaffolds were prepared 85% porosity, diameters ranges 470–590, 590–850 850–1200 µm. Rat marrow stem cells differentiated into osteoblasts cultured up 10 days growth,...
Abstract One of the strategies to improve mechanical performance bioactive glasses for load‐bearing implant devices has been development glass‐ceramic materials. The present study aimed evaluate effect a highly bioactive, fully‐crystallized (Biosilicate®) system P 2 O 5 –Na O–CaO–SiO on various key parameters in vitro osteogenesis. Surface characterization was carried out by scanning electron microscopy and Fourier transform infrared spectroscopy. Osteogenic cells were obtained enzymatic...
The aim of this study was to investigate if chemically produced nanotopography on titanium (Ti) surface induces osteoblast differentiation cultured human bone marrow mesenchymal stem cells (hMSCs) by regulating the expression microRNAs (miRs). It demonstrated that Ti with hMSCs as evidenced upregulation specific markers compared untreated (control) at day 4. At time-point, miR-sequencing analysis revealed 20 miRs were upregulated (>twofold) while downregulated (>threefold) in grown control...
ABSTRACT The aim of our study was to investigate the osteoinductive potential a titanium (Ti) surface with nanotopography, using mesenchymal stem cells (MSCs) and mechanism involved in this phenomenon. Polished Ti discs were chemically treated H 2 SO 4 /H O yield nanotopography rat MSCs cultured under osteogenic non‐osteogenic conditions on both untreated polished (control) surfaces. increased cell proliferation alkaline phosphatase (Alp) activity upregulated gene expression key bone markers...
The investigation of titanium (Ti) surface modifications aiming to increase implant osseointegration is one the most active research areas in dental implantology. This study was carried out evaluate benefits coating Ti with type I collagen on implants. Acid etched implants (AETi), either untreated or coated (ColTi), were placed dog mandibles for three and eight weeks histomorphometric, cellular molecular evaluations bone tissue response. While histological aspects essentially same both being...
ABSTRACT We aimed at evaluating the effect of titanium (Ti) with nanotopography (Nano) on endogenous expression BMP‐2 and BMP‐4 relevance this process to nanotopography‐induced osteoblast differentiation. MC3T3‐E1 cells were grown Nano machined (Machined) Ti surfaces BMP‐2/4 BMP receptor BMPR1A silencing in both differentiation genes related TGF‐β/BMP signaling evaluated. supported higher gene protein upregulated compared Machined surface. The inhibited osteogenic potential induced by...
This study evaluated the impact of a single variation in etching time H2SO4/H2O2-treated titanium (Ti) surfaces on adsorption growth and differentiation factor-5 (GDF-5) their effects acquisition osteogenic phenotype vitro. Rat primary calvarial cells were grown for up to 14 days following Ti surfaces: (1) 30 min: nanotopography obtained with 1:1 mixture H2SO4/H2O2 min (control); (2) + GDF-5: min-etched sample adsorbed recombinant human (rh) GDF-5; (3) 4 h: h (4) h-etched rhGDF-5. The GDF-5...
Abstract: The study investigates the biocompatibility of porcine acellular dermal matrix (PADM) as a tissue substitute in periodontal applications. Given limitations associated with subepithelial connective grafts (SCTG), PADM emerges promising al-ternative for integration and wound healing. research employed histological analyses rat model to evaluate PADM’s over 7, 14, 21, 28 days. surgical procedure involved subcutaneous implantation, followed by histomorphomet-rical analysis fibrous...
Abstract Background: It has recently been reported that controlled chemical oxidation of titanium (Ti) with sulfuric acid (H 2 SO 4 )/hydrogen peroxide O ) significantly influences the early stages in vitro osteogenesis. The aim this study was to evaluate whether treatment can also influence vivo bone formation. Material and methods: Ti implants (Mk III) were etched H /H for h at room temperature. Mandibular premolars extracted eight dogs and, after 3 months, three treated untreated placed...
Abstract Objective: This study aimed at investigating the influence of porous titanium (Ti) structure on osteogenic cell behaviour. Materials and methods: Porous Ti discs were fabricated by powder metallurgy process with pore size typically between 50 400 μm a porosity 60%. Osteogenic cells obtained from human alveolar bone cultured until subconfluence subcultured dense (control) for periods up to 17 days. Results: Cultures grown exhibited increased proliferation total protein content, lower...
The aim of this study was to investigate the effects low-level laser therapy (LLLT) by using gallium aluminum arsenide (GaAlAs) diode on human osteoblastic cells grown titanium (Ti). Osteoblastic were obtained enzymatic digestion alveolar bone and cultured Ti discs for up 17 days. Cells exposed LLLT at 3 J/cm2 (wavelength 780 nm) days 7 non-irradiated cultures used as control. treatment did not influence culture growth, ALP activity, mineralized matrix formation. Analysis epifluorescence...
Osteogenic cells express some matrix proteins at early culture intervals. The aim of this study was to determine if, and in what proportion, used for plating contain bone sialoprotein (BSP) osteopontin (OPN), two associated with initial events formation. Their pattern expression, as well that fibronectin (FN) type I pro-collagen, also examined 6 hr 1 3 days. were obtained by enzymatic digestion newborn rat calvariae, grown on glass coverslips. Cytocentrifuge preparations isolated coverslips...
This study was aimed at investigating the in vitro biocompatibility of a novel membrane composite poly(vinylidene-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT). Osteoblastic cells were obtained from human alveolar bone fragments and cultured under standard osteogenic condition until subconfluence. First passaged on P(VDF-TrFE)/BT expanded polytetrafluoroethylene (e-PTFE--control) membranes 24-well plates. Cell adhesion spreading evaluated 30 min, 4 24 h. For proliferation assay, for 1,...
Abstract Objectives: The aims of this research were to evaluate the efficacy a bioactive glass–ceramic (Biosilicate ® ) and glass (Biogran placed in dental sockets maintenance alveolar ridge osseointegration Ti implants. Material methods: Six dogs had their low premolars extracted implanted with Biosilicate , Biogran particles, or left untreated. After extractions, measurements width height on taken. 12 weeks new surgery was performed take final insert bilaterally three implants...