A5073326828- Peripheral Neuropathies and Disorders
- MicroRNA in disease regulation
- Myasthenia Gravis and Thymoma
- Inflammatory Myopathies and Dermatomyositis
- RNA modifications and cancer
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- Nanoparticle-Based Drug Delivery
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Long-Term Effects of COVID-19
- Cancer-related molecular mechanisms research
- Cancer Treatment and Pharmacology
- Reproductive Biology and Fertility
- Endoplasmic Reticulum Stress and Disease
- Neurogenetic and Muscular Disorders Research
- RNA Research and Splicing
- Chronic Lymphocytic Leukemia Research
- Neurogenesis and neuroplasticity mechanisms
- Parkinson's Disease and Spinal Disorders
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Genetics and Reproduction
- Multiple Myeloma Research and Treatments
Yale University
2018-2025
University of Alabama at Birmingham
2016-2025
Mayo Clinic in Florida
2023-2025
Chinese Academy of Sciences
2024-2025
Jacksonville College
2025
Sanofi (Belgium)
2024
WinnMed
2019-2024
Mayo Clinic in Arizona
2013-2024
Mayo Clinic
2013-2024
National Dairy Research Institute
2011-2024
<h3>Importance</h3> Many patients with generalized myasthenia gravis (gMG) have substantial clinical disability, persistent disease burden, and adverse effects attributable to chronic immunosuppression. Therefore, there is a significant need for targeted, well-tolerated therapies the potential improve control enhance quality of life. <h3>Objective</h3> To evaluate zilucoplan, subcutaneously (SC) self-administered macrocyclic peptide inhibitor complement component 5, in broad population...
Nanoscale drug delivery vehicles have been harnessed extensively as carriers for cancer chemotherapeutics. However, traditional pharmaceutical approaches nanoformulation a challenge with molecules that exhibit incompatible physicochemical properties, such platinum-based Here we propose paradigm based on rational design of active facilitate supramolecular assembly in the nanoscale dimension. Using cisplatin template, describe synthesis unique platinum (II) tethered to cholesterol backbone via...
Abstract Stress plays a major role in inducing depression, which may arise from interplay between complex cascades of molecular and cellular events that influence gene expression leading to altered connectivity neural plasticity. In recent years, microRNAs (miRNAs) have carved their own niche owing innate ability induce disease phenotype by regulating large number genes cohesive coordinated manner. this study, we examined whether miRNAs associated networks chronic corticosterone (CORT; 50 mg...
During the COVID-19 pandemic, patients with neuromuscular disorders, especially autoimmune myasthenia gravis, might be at greater risk of worse outcomes than otherwise healthy people because an immunocompromised state related to immunotherapy and possible respiratory bulbar muscular weakness. However, cessation in neuroinflammatory disorders has severe risks as well.1Korsukewitz C Reddel SW Bar-Or A Wiendl H Neurological era COVID-19—looking for consensus literature.Nat Rev Neurol. 2020; 16:...
Objective: Proinflammatory cytokines have recently received considerable attention for their role in suicidal behavior; however, how the expression of cytokine genes is regulated not clearly known. The authors examined underlying mechanisms critical gene tumor necrosis factor–alpha (TNF-α) dysregulation brains individuals who died by suicide. Method: TNF-α was dorsolateral prefrontal cortex postmortem persons with and without major depressive disorder suicide causes other than putative...
Autoantibodies targeting the acetylcholine receptor (AChR), found in patients with myasthenia gravis (MG), mediate pathology through 3 mechanisms: complement-directed tissue damage, blocking of binding site, and internalization AChR. Clinical assays, used to diagnose monitor patients, measure only autoantibody binding. Consequently, they are limited providing association disease burden, understanding mechanistic heterogeneity, monitoring therapeutic response. The objective this study was...
Abstract INTRODUCTION Loss‐of‐function variants of the ABCA7 gene are associated with an increased risk Alzheimer's disease (AD). How neuronal contributes to AD pathogenesis is unknown. METHODS Using neuron‐specific Abca7 KO mice (n −/− ) or without 5×FAD amyloid model background and post mortem brains, we investigated AD‐related phenotypes through comprehensive approaches including transcriptomics lipidomics. RESULTS Lipidomics analysis detected altered lipid profiles in brains synaptosomes...
We report the establishment of an efficient and heritable gene mutagenesis method in silkworm Bombyx mori using modified type II clustered regularly interspaced short palindromic repeats (CRISPR) with associated protein (Cas9) system. Using four loci Bm-ok, BmKMO, BmTH, Bmtan as candidates, we proved that genome alterations at specific sites could be induced by direct microinjection guide RNA Cas9-mRNA into embryos. Mutation frequencies 16.7-35.0% were observed injected generation, DNA...
Abstract Norepinephrine (NE) is produced primarily by neurons in the locus coeruleus (LC). Retrograde and ultrastructural examinations reveal that core of LC its surrounding region receives afferent projections from several brain areas which provide multiple neurochemical inputs to with changes neuronal firing, making it a highly coordinated event. Although NE mediated signaling systems have been studied relation suicide psychiatric disorders increase risk including depression, less known...
The aim of this study was to investigate if chemically produced nanotopography on titanium (Ti) surface induces osteoblast differentiation cultured human bone marrow mesenchymal stem cells (hMSCs) by regulating the expression microRNAs (miRs). It demonstrated that Ti with hMSCs as evidenced upregulation specific markers compared untreated (control) at day 4. At time-point, miR-sequencing analysis revealed 20 miRs were upregulated (>twofold) while downregulated (>threefold) in grown control...
Abstract Altered synaptic plasticity is often associated with major depressive disorder (MDD). Disease-associated changes in functions are tightly correlated altered microRNA (miRNA) expression. Here, we examined the role of miRNAs and their functioning at synapse MDD by examining miRNA processing machinery sequencing analyzing total tissue fractions obtained from dorsolateral prefrontal cortex (dlPFC) 15 matched non-psychiatric control subjects. A 333 were reliably detected fraction....
Dissemination of ovarian cancer cells can lead to inoperable metastatic lesions in the bowel and omentum that cause patient death. Here we show LRRC15, a type-I 15-leucine-rich repeat-containing membrane protein, highly overexpressed metastases compared with matched primary tumors acts as potent promoter omental metastasis. Complementary models demonstrated LRRC15 expression leads inhibition anoikis-induced cell death promotes adhesion invasion through matrices mimic omentum....
Abstract ABCA7 loss-of-function variants are associated with increased risk of Alzheimer’s disease (AD). Using knockout human iPSC models generated CRISPR/Cas9, we investigated the impacts deficiency on neuronal metabolism and function. Lipidomics revealed that mitochondria-related phospholipids, such as phosphatidylglycerol cardiolipin were reduced in ABCA7-deficient iPSC-derived cortical organoids. Consistently, deficiency-induced alterations mitochondrial morphology accompanied by ATP...
The immune system substantially influences age-related cognitive decline and Alzheimer's disease (AD) progression, affected by genetic environmental factors. In a Mayo Clinic Study of Aging cohort, we examined how risk factors like APOE genotype, age, sex affect inflammatory molecules AD biomarkers in cerebrospinal fluid (CSF). Among cognitively unimpaired individuals over 65 (
The ability of embryonic stem cells to differentiate into endothelium and form functional blood vessels has been well established can potentially be harnessed for therapeutic angiogenesis. However, after almost two decades investigation in this field, limited knowledge exists directing endothelial differentiation. A better understanding the cellular mechanisms regulating vasculogenesis is required development cell-based models therapies. In study, we elucidated mechanistic role insulin-like...
Venous neointimal hyperplasia (VNH) causes hemodialysis vascular access failure. Here we tested whether VNH formation occurs in part due to local vessel hypoxia caused by surgical trauma the vasa vasorum of outflow vein at time arteriovenous fistula placement. Selective targeting adventitia creation was performed using a lentivirus-delivered small-hairpin RNA that inhibits VEGF-A expression. This resulted significant increase mean lumen area, decreased media/adventitia and constrictive...