A5100323812- Cancer-related molecular mechanisms research
- Hearing, Cochlea, Tinnitus, Genetics
- Neuroinflammation and Neurodegeneration Mechanisms
- Thyroid Disorders and Treatments
- Vestibular and auditory disorders
- Effects and risks of endocrine disrupting chemicals
- CRISPR and Genetic Engineering
- Hearing Loss and Rehabilitation
- Neurological Disease Mechanisms and Treatments
- MicroRNA in disease regulation
- RNA modifications and cancer
- Birth, Development, and Health
- RNA Research and Splicing
- Circular RNAs in diseases
- Anesthesia and Neurotoxicity Research
- Growth Hormone and Insulin-like Growth Factors
- Ophthalmology and Eye Disorders
- Neuroscience and Neuropharmacology Research
- Neonatal Health and Biochemistry
- Diet, Metabolism, and Disease
- Extracellular vesicles in disease
- Immune cells in cancer
- RNA regulation and disease
- Obesity, Physical Activity, Diet
- Neuroscience of respiration and sleep
Chengdu Third People's Hospital
2025
Southwest Jiaotong University
2025
China Medical University
2014-2024
Massachusetts Eye and Ear Infirmary
2022-2024
Harvard University
2022-2024
Zhongnan Hospital of Wuhan University
2023-2024
Wuhan University
2023-2024
Jilin University
2024
Xijing Hospital
2021-2023
Fujian University of Traditional Chinese Medicine
2017-2023
Abstract Systemic transplantation of oxygen−glucose deprivation (OGD)-preconditioned primary microglia enhances neurological recovery in rodent stroke models, albeit the underlying mechanisms have not been sufficiently addressed. Herein, we analyzed whether or extracellular vesicles (EVs) derived from such are biological mediators these observations and which signaling pathways involved process. Exposing bEnd.3 endothelial cells (ECs) cortical neurons to OGD, impact EVs OGD-preconditioned on...
ABSTRACT Accumulating evidence demonstrates that acetylcholine can directly modulate immune function in peripheral tissues including the spleen and gastrointestinal tract. However, anatomical relationships between cholinergic system cells located these lymphoid remain unclear due to inherent technical difficulties with currently available neuroanatomical methods. In this study, mice specific expression of tdTomato fluorescent protein choline acetyltransferase (ChAT)‐expressing were used...
Outer membrane vesicles (OMVs) are nanosized produced by the gut microbiota (GM). The GM is well-known to be involved in pathological process of Alzheimer's disease (AD). However, mechanism OMVs not clear. In present study, we demonstrated involvement development cognitive (learning and memory) dysfunction induced blood-brain barrier (BBB) disruption. More important, further study showed that tau phosphorylation activating glycogen synthase kinase 3β (GSK-3β) hippocampus. activated...
Abstract Background The vast majority of trait-associated variants identified using genome-wide association studies (GWAS) are noncoding, and therefore assumed to impact gene regulation. However, the loci unexplained by regulatory quantitative trait (QTLs). Results We perform a comprehensive characterization putative mechanisms which GWAS human immune traits. By harmonizing four major QTL studies, we identify 26,271 expression QTLs (eQTLs) 23,121 splicing (sQTLs) spanning 18 cell types. Our...
Ginsenoside Rg1 is the principal active ingredient in ginseng. The antidepressant effects of have been validated; however, specific underlying mechanism this effect needs further research. Rats were subjected to chronic restraint stress (CRS) depression model. Rg1, or a positive control drug, was administered rats. Depression-like behaviours evaluated through behavioural experiments. Cytokine, mRNA, protein, ATP, and mitochondria DNA levels detected using indicated methods....
Background: Stroke stimulates reactive astrogliosis, aquaporin 4 (AQP4) depolarization and neuroinflammation.Preconditioned extracellular vesicles (EVs) from microglia exposed to hypoxia, in turn, reduce poststroke brain injury.Nevertheless, the underlying mechanisms of such effects are elusive, especially with regards inflammation, AQP4 polarization, cerebrospinal fluid (CSF) flow.Methods: Primary astrocytes were oxygen-glucose deprivation (OGD) injury.For analyzing role expression patterns...
We report the establishment of an efficient and heritable gene mutagenesis method in silkworm Bombyx mori using modified type II clustered regularly interspaced short palindromic repeats (CRISPR) with associated protein (Cas9) system. Using four loci Bm-ok, BmKMO, BmTH, Bmtan as candidates, we proved that genome alterations at specific sites could be induced by direct microinjection guide RNA Cas9-mRNA into embryos. Mutation frequencies 16.7-35.0% were observed injected generation, DNA...
Strategies to overcome irreversible cochlear hair cell (HC) damage and loss in mammals are of vital importance hearing recovery patients with permanent loss. In mature mammalian cochlea, co-activation Myc Notch1 reprograms supporting cells (SC) promotes HC regeneration. Understanding the underlying mechanisms may aid development a clinically relevant approach achieve regeneration nontransgenic cochlea. By single-cell RNAseq, we show that MYC/NICD “rejuvenates” adult mouse cochlea by...
Mutations in microRNA-96 ( MIR96 ) cause autosomal dominant deafness-50 (DFNA50), a form of delayed-onset hearing loss. Genome editing has shown efficacy recovery through intervention neonatal mice, yet the adult inner ear is necessary for clinical applications, which not been done. Here, we developed genome therapy mutation 14C>A by screening different CRISPR systems and optimizing Cas9 expression sgRNA scaffold efficient specific editing. AAV delivery KKH variant Staphylococcus aureus...
Sound activates not only the cochlea but also vestibular end organs. Research on this phenomenon led to discovery of sound-evoked myogenic potentials recorded from sternocleidomastoid muscles (cervical VEMP, or cVEMP). Since cVEMP offers simplicity and ability stimulate each labyrinth separately, its values as a test human function are widely recognized. Currently, is interpreted saccule based assumption that clicks primarily activate saccule. However, sound activation organs other than has...
The main object of the present study was to explore effect perinatal bisphenol A (BPA) exposure on glucose metabolism in early and later life male rat offspring, establish potential mechanism BPA-induced dysglycemia. Pregnant rats were treated with either vehicle or BPA by drinking water at concentrations 1 10 µg/mL from gestation day 6 through end lactation. We measured levels fasting serum glucose, insulin, adiponectin parameters oxidative stress postnatal (PND) 50 PND100 mRNA protein...
To investigate the possible effects of anxiety and/or depression symptoms on treatment outcomes and recurrence benign paroxysmal positional vertigo (BPPV).This is a retrospective study conducted at single institution. 142 consecutive patients diagnosed with idiopathic BPPV Department Otology in Shengjing Hospital China Medical University between October 2016 July 2017 were retrospectively reviewed. 127 finally included this study. Zung self-rating scale (SAS) (SDS) used to evaluate presence...
The prevalence, characteristics, and trends in obesity, overweight, malnutrition among children adolescents 2010 2014 Shenyang, China was described.This a multiple cross-sectional study using data from the National Survey on Students' Constitution Health. A total of 31,031 were included this survey. Differences percentages by age, gender, living region compared χ2 test. Stepwise logistic regression performed to select potential covariates for dependent variable (overweight, or...
Developmental iodine deficiency results in inadequate thyroid hormone (TH), which damages the hippocampus. Here, we explored roles of hippocampal doublecortin and neural cell adhesion molecule (NCAM)-180 developmental hypothyroidism.Two rat models were established with either an iodine-deficient diet, or propylthiouracil (PTU)-adulterated water (5 ppm 15 ppm) to impair function, pregnant rats from gestational day 6 until postnatal (PN) 28. Silver-stained neurons protein levels NCAM-180...