A5101744514- Silk-based biomaterials and applications
- Neurobiology and Insect Physiology Research
- Insect Utilization and Effects
- Silkworms and Sericulture Research
- Viral Infectious Diseases and Gene Expression in Insects
- Invertebrate Immune Response Mechanisms
- Insect Resistance and Genetics
- Insect and Arachnid Ecology and Behavior
- Studies on Chitinases and Chitosanases
- Biofuel production and bioconversion
- Insect and Pesticide Research
- Insect-Plant Interactions and Control
- RNA Interference and Gene Delivery
- Aquaculture disease management and microbiota
- CRISPR and Genetic Engineering
- Microbial Metabolic Engineering and Bioproduction
- Physiological and biochemical adaptations
- Insect symbiosis and bacterial influences
- Coleoptera: Cerambycidae studies
- 3D Printing in Biomedical Research
- Signaling Pathways in Disease
- bioluminescence and chemiluminescence research
- Gout, Hyperuricemia, Uric Acid
- Entomopathogenic Microorganisms in Pest Control
- RNA Research and Splicing
South China University of Technology
2024
Zhejiang University
2010-2020
Wyoming Department of Education
2010
University of Wyoming
2010
Shizuoka University
2009
Institute of Animal Sciences
2007
Ōtani University
2006
Xiaomi (China)
2005
The development of a spider silk-manufacturing process is great interest. However, there are serious problems with natural manufacturing through farming, and standard recombinant protein production platforms have provided limited progress due to their inability assemble silk proteins into fibers. Thus, we used piggyBac vectors create transgenic silkworms encoding chimeric silkworm/spider proteins. fibers produced by these animals were composite materials that included integrated in an...
Abstract MicroRNAs (miRNAs) are endogenous small non‐coding RNAs regulating gene expression in animals and plants. To find some differentially expressed miRNAs that may be associated with age‐dependent behavioural changes honey bees ( Apis mellifera ), we applied next‐generation high‐throughput sequencing technology to detect nurses foragers. Our results showed both foragers had a complicated RNA population, the length of varied, 22 nucleotides being predominant length. Combining deep...
We report the establishment of an efficient and heritable gene mutagenesis method in silkworm Bombyx mori using modified type II clustered regularly interspaced short palindromic repeats (CRISPR) with associated protein (Cas9) system. Using four loci Bm-ok, BmKMO, BmTH, Bmtan as candidates, we proved that genome alterations at specific sites could be induced by direct microinjection guide RNA Cas9-mRNA into embryos. Mutation frequencies 16.7-35.0% were observed injected generation, DNA...
Bombyx mori (B. mori) silk fibroin and sericin can act as a great candidate in delivering drugs or other bioactive substances. Silica also has application the field of drug delivery. To best our knowledge, there been no report on design nanocomposite made protein silica for Here, first time, we used B. (SF) (SS), self-assembled into nanospheres nanofibers situ aqueous solution, respectively, biotemplate to regulate nucleation self-assembly designing anticancer SF SS mediated assembly...
We utilized the proteomic approach to investigate proteome of fifth instar hemolymph during growth and development, improve understanding this important bioprocess gene expression situation. A total 25 μL was used for 2D analysis, separated proteins were visualized by silver staining analyzed using ImageMaster software. The report showed as many 241 protein spots expressed in beginning instar. Among them, most concentrated pI 3.5−6.5, which reached 76% spots. As molecular sizes, 182 between...
The effects of several factors on the activity alkaline phosphatase (ALKP) in midgut silkworm, Bombyx mori L were studied. ALKP varied greatly among tested strains. There was positive correlation between and cocoon quality. decreased when silkworm infected with cytoplasmic polyhedrosis virus (CPV); whereas, infection nuclear (NPV) did not affect activity. This suggests that pathogenic mechanism CPV differs from NPV. Bacillus thuringiensis caused direct damage to tissues a rapid decline...
Salivary gland secretion is altered in Drosophila embryos with loss of function the sage gene. Saliva has a reduced volume and an increased electron density according to transmission microscopy, resulting regions tube dilation constriction intermittent closure. However, precise functions Bmsage silkworm (Bombyx mori) are unknown, although its sequence had been deposited SilkDB. From this, inferred be transcription factor that regulates synthesis silk fibroin interacts another gland-specific...
Abstract Ecdysteroids play a critical role in coordinating insect growth, development and reproduction. A suite of cytochrome P450 monooxygenases coded by what are collectively termed Halloween genes mediate ecdysteroid biosynthesis. In this study, we describe cloning RNA interference (RNAi)‐mediated knockdown the CYP307B1 gene ( Spookiest ) western tarnished plant bug, Lygus hesperus . Transcripts for Ly. LhSpot were amplified from all life stages correlated well with timing pre‐moult...
Hedgehog (Hh) signals regulate invertebrate and vertebrate development, yet the role of pathway in adipose development remains poorly understood. In this report, we found that Hh components are expressed fat body silkworm larvae. Functional analysis these a BmN cell line model revealed activation gene stimulated transcription components, but inhibited expression marker AP2. Conversely, specific RNA interference-mediated knockdown resulted increased AP2 expression. This further showed...
Abstract PLA2 enzyme hydrolyzes arachidonic acid, and other polyunsaturated fatty acids, from the sn‐2 position to release free acid a lysophospholipid. Previous studies reported that in invertebrate organisms participates lipid signaling molecules like immune‐associated tissues hemocytes fat bodies. In present study, we cloned BmPLA2 gene body tissue of silkworm Bombyx mori , which has total sequence 1.031 kb with 31.90 kDa protein. silico results Bm indicated protein putative WD40...
Abstract The physiological titer of molting hormones in insects depends on relative activities synthesis and degradation pathways. Ecdysone oxidase (EO) is a key enzyme the inactivation ecdysteroid. However, there are only few reports ecdysteroid its enzymes silkworm. In this study, we cloned characterized Bombyx mori EO (BmEO). BmEO cDNA contains an ORF 1,695 bp deduced protein sequence 564 amino acid residues. two functional domains glucose–methanol–choline oxidoreductase N‐terminal...
Abstract Molting in insects is regulated by molting hormones (ecdysteroids), which are also crucial to insect growth, development, reproduction, etc. Ecdysone was inactivated 3‐dehydroecdysone (3DE) under ecdysone oxidase (EO), and followed NAD(P)H‐dependent irreversible reduction 3‐epiecdysteroid 3DE 3a‐reductase. On the other hand, undergoes reversible 3 β ‐reductase hemolymph. In this article, we cloned characterized (3DE ‐reductase) different tissues developing stage from silkworm,...