A5032324140- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Cellular Mechanics and Interactions
- Protease and Inhibitor Mechanisms
- Cancer, Hypoxia, and Metabolism
- Polyamine Metabolism and Applications
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Cancer, Stress, Anesthesia, and Immune Response
- Advanced Proteomics Techniques and Applications
- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Amino Acid Enzymes and Metabolism
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- FOXO transcription factor regulation
- Cytokine Signaling Pathways and Interactions
- RNA Research and Splicing
- TGF-β signaling in diseases
- Cancer Genomics and Diagnostics
- COVID-19 epidemiological studies
- Mechanisms of cancer metastasis
- PI3K/AKT/mTOR signaling in cancer
Harvard University
2015-2025
Boston Children's Hospital
2015-2025
Children's Hospital
1988-2023
Center for Vascular Biology Research
2015-2023
Boston Children's Museum
2009-2020
University of California, Los Angeles
2015
University of Vermont
2015
Institute for Molecular Medicine
2015
John Wiley & Sons (United States)
2015
Hudson Institute
2015
Acetylated-low density lipoprotein (Ac-LDL) is taken up by macrophages and endothelial cells via the "scavenger cell pathway" of LDL metabolism. In this report, aortic microvascular internalized degraded 7-15 times more [125I]-Ac-LDL than did smooth muscle or pericytes. Bound was displaced unlabeled Ac-LDL, but not unmodified LDL. The ability to identify based on their increased metabolism Ac-LDL examined using labeled with fluorescent probe...
Capillary endothelial cells from rats, calves, and humans, have been carried in long-term culture. Bovine capillary cloned maintained by serial passage for longer than 8 months. This prolonged culture was accomplished using tumor-conditioned medium, gelatin-coated plates, a method of enriching primary Cultured bovine produce Factor VIII antigen angiotensin-converting enzyme, but do not Weibel-Palade bodies. Human contain are distinguished aortic their requirement conditioned medium. regular...
Migration of capillary endothelial cells is an important component angiogenesis in vivo. Increased numbers mast have been associated with several types angiogenesis. We used a quantitative assay vitro to demonstrate that release factor significantly increases bovine cell migration. The present medium conditioned by as well lysates cells. stimulatory effect on migration specific for Furthermore, no mitogenic activity Of all the secretory products tested, only heparin stimulated vitro. Heparin...
Interferon derived from human leukocytes, fibroblasts, and mouse fibroblasts was found to inhibit the motility of cultured cells. It inhibits tumor-induced capillary endothelial cells as well spontaneous migration other cell types. The ability a given preparation interferon type is proportional its antiviral activity in that particular type. Antiserum leukocyte neutralizes both motility-inhibitory this preparation.
Pancreatic cancer, one of the deadliest human malignancies, is almost invariably associated with presence an oncogenic form Kras. Mice expressing Kras in pancreas recapitulate stepwise progression disease. The inflammatory cytokine interleukin (IL)-6 often expressed by multiple cell types within tumor microenvironment. Here, we show that IL-6 required for maintenance and pancreatic cancer precursor lesions. In fact, lack completely ablates even Mechanistically, synergizes to activate...
RNA interference (RNAi) represents a promising strategy for identification and validation of putative therapeutic targets treatment myriad important human diseases including cancer. However, the effective systemic in vivo delivery small interfering (siRNA) to tumors remains formidable challenge. Using robust self-assembly strategy, we develop unique nanoparticle (NP) platform composed solid polymer/cationic lipid hybrid core lipid-poly(ethylene glycol) (lipid-PEG) shell siRNA delivery. The...
Angiostatin, a fragment of plasminogen, has been identified and characterized as an endogenous inhibitor neovascularization. We show that angiostatin treatment endothelial cells in the absence growth factors results increased apoptotic index whereas proliferation is unchanged. Angiostatin also inhibits migration tube formation cells. no effect on factor-induced signal transduction but leads to RGD-independent induction kinase activity focal adhesion kinase, suggesting biological effects...
Cloned integrin α 2 subunit complementary DNA was expressed on human rhabdomyosarcoma (RD) cells to give a functional VLA-2 (α β 1 ) adhesion receptor. The VLA-2-positive RDA2 not only showed increased collagen and laminin in vitro, but also formed substantially more metastatic tumor colonies nude mice after either intravenous or subcutaneous injection. These results show that specific receptor (VLA-2) can markedly enhance both experimental spontaneous metastasis. In contrast the metastasis...
Neovascularization is a feature of variety pathological processes. We compared the characteristics vascular endothelial growth factor (VEGF) and basic fibroblast (bFGF) on migration proliferation human umbilical vein endothelium (HUVEC). Both VEGF bFGF induced cell at similar concentrations (½ max. = ∼1.0 ng/ml, ∼5.0 ng/ml). However, VEGF-stimulated was two-fold greater than 1 10 ng/ml (p < 0.05). In contrast, four-fold more effectively 1.4 ng/ ml respectively). Checkerboard assays for...
Microarrays have been the primary means for large-scale analyses of genes implicated in cancer progression. However, more recently a need has recognized investigating development directly at protein level. In this report, we applied comparative proteomic technique to study metastatic prostate cancer. This technology, termed stable isotope labeling with amino acids cell culture (SILAC), gained popularity its ability compare expression levels hundreds proteins single experiment. SILAC makes...
Messenger RNA (mRNA) has recently emerged with remarkable potential as an effective alternative to DNA-based therapies because of several unique advantages. mRNA does not require nuclear entry for transfection activity and a negligible chance integrating into the host genome which excludes possibility potentially detrimental genomic alternations. Chemical modification further enhanced its stability decreased activation innate immune responses. Additionally, been found have rapid expression...