A5046999994- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- Nanoplatforms for cancer theranostics
- Innovative Microfluidic and Catalytic Techniques Innovation
- PI3K/AKT/mTOR signaling in cancer
- interferon and immune responses
- Clinical Nutrition and Gastroenterology
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Dendrimers and Hyperbranched Polymers
- Advanced Cellulose Research Studies
- Immune cells in cancer
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Digestive system and related health
- Nanomaterials for catalytic reactions
- Biosensors and Analytical Detection
- Monoclonal and Polyclonal Antibodies Research
- Nanoparticle-Based Drug Delivery
- RNA Research and Splicing
- Extracellular vesicles in disease
- Adenosine and Purinergic Signaling
Harvard University
2017-2025
Brigham and Women's Hospital
2017-2025
ETH Zurich
2023
Inspire
2020
University of Waterloo
2015-2018
Breast cancer develops from local tissue but is characterized by a distinct metastatic pattern involving regional lymph nodes and distant organs, which the primary cause of high mortality in breast patients. Herein, optimal docking nanoparticles (NPs) composed laurate-functionalized Pt(IV) prodrug (Pt(lau)), human serum albumin (HSA), lecithin were predicted computational modeling, prepared nanoprecipitation, validated fluorescence spectroscopy. As macrophages have been reported to be...
Generation 6.0 polyamidoamine (G6 PAMAM) dendrimer-grafted cellulose nanocrystals (CNCs) (CNC–PAMAM) were synthesized and employed as supports for gold nanoparticles. The successful grafting of PAMAM dendrimers was confirmed by conductometric–potentiometric titration pH-dependent ζ-potential analyses. Gold nanoparticles with diameters approximately 2 to 4 nm the playing role nanoreactors NaBH4 reducing agent. More importantly, successfully prepared at pH 3.3 functional Temperature...
Many patients infected with coronaviruses, such as SARS-CoV-2 and NL63 that use ACE2 receptors to infect cells, exhibit gastrointestinal symptoms viral proteins are found in the human tract, yet little is known about inflammatory pathological effects of coronavirus infection on intestine. Here, we used a intestine-on-a-chip (Intestine Chip) microfluidic culture device lined by patient organoid-derived intestinal epithelium interfaced vascular endothelium study host cellular responses...
Abstract Environmental enteric dysfunction (EED)—a chronic inflammatory condition of the intestine—is characterized by villus blunting, compromised intestinal barrier function and reduced nutrient absorption. Here we show that essential genotypic phenotypic features EED-associated injury can be reconstituted in a human intestine-on-a-chip lined organoid-derived epithelial cells from patients with EED cultured nutrient-deficient medium lacking niacinamide tryptophan. Exposure organ chip to...
The rising threat of pandemic viruses, such as SARS-CoV-2, requires development new preclinical discovery platforms that can more rapidly identify therapeutics are active in vitro and also translate vivo . Here we show human organ-on-a-chip (Organ Chip) microfluidic culture devices lined by highly differentiated primary lung airway epithelium endothelium be used to model virus entry, replication, strain-dependent virulence, host cytokine production, recruitment circulating immune cells...
The current coronavirus disease 2019 (COVID-19) pandemic highlights the need for broad-spectrum antiviral therapeutics. Here we describe a new class of self-assembling immunostimulatory short duplex RNAs that potently induce production type I and III interferon (IFN-I IFN-III). These require minimum 20 base pairs, lack any sequence or structural characteristics known RNAs, instead unique motif (sense strand, 5'-C; antisense 3'-GGG) mediates end-to-end dimer self-assembly. presence terminal...
Oxidative stress accompanying the reactive oxygen species (ROS) burst governs immunocyte infiltration, activation, and differentiation in tumor microenvironments thus can elicit robust antitumor immunity. Here, we identify a photoactive metal–organic coordination polymer (MOCP), composed of an organometallic core formed by cytotoxic mitoxantrone (MTX) acylates photosensitive Ru(BIQ)-HDBB [BIQ = 2,2′-biquinoline, HDBB 4,4′-di(4-benzoato)-2,2′-bipyridine] linked Fe(II) ions via coordinate...
We developed and characterized a biologically functional microcirculation system consisting of arterial venous endothelial cells pericytes. The model was employed to study leukocyte transendothelial migration in the healthy inflamed state.
The current COVID-19 pandemic highlights the need for broad-spectrum antiviral therapeutics. Here we describe a new class of self-assembling immunostimulatory short duplex RNAs that potently induce production type I and III interferon (IFN-I IFN-III), in wide range human cell types. These require minimum 20 base pairs, lack any sequence or structural characteristics known RNAs, instead unique conserved motif (sense strand: 5'-C, antisense 3'-GGG) mediates end-to-end dimer self-assembly these...
11582 Background: The onset and maintenance of cancer frequently involves gain oncogenic function along with loss tumor suppression. PTEN is a well-characterized suppressor gene that lost or mutated in many human cancers including ~50% metastatic castration-resistant prostate (mCRPC). Reintroduction functional for mCRPC treatment has proven difficult. Methods: mRNA was synthesized by vitro transcription method modified ARCA capping enzymatic polyadenylation, then substituted Pseudo-UTP,...
ABSTRACT Environmental Enteric Dysfunction (EED) is a chronic inflammatory condition of the intestine characterized by villus blunting, compromised intestinal barrier function, and reduced nutrient absorption. Here, we show that key genotypic phenotypic features EED-associated injury can be reconstituted in human intestine-on-a-chip (Intestine Chip) microfluidic culture device lined organoid-derived epithelial cells from EED patients cultured niacinamide- tryptophan-deficient (-N/-T) medium....
Abstract Many patients infected with coronaviruses, such as SARS-CoV-2 and NL63 that use ACE2 receptors to infect cells, exhibit gastrointestinal symptoms viral proteins are found in the human tract, yet little is known about inflammatory pathological effects of coronavirus infection on intestine. Here, we used a intestine-on-a-chip (Intestine Chip) microfluidic culture device lined by patient organoid-derived intestinal epithelium interfaced vascular endothelium study host cellular...