A5083353329- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Inhalation and Respiratory Drug Delivery
- Industrial Technology and Control Systems
- Advanced machining processes and optimization
- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Manufacturing Process and Optimization
- Advanced Surface Polishing Techniques
- Simulation and Modeling Applications
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory viral infections research
- Long-Term Effects of COVID-19
- Advanced Machining and Optimization Techniques
- COVID-19 Clinical Research Studies
- Microfluidic and Bio-sensing Technologies
- Tracheal and airway disorders
- interferon and immune responses
- Ion Transport and Channel Regulation
- Protein Degradation and Inhibitors
- Advanced biosensing and bioanalysis techniques
- Congenital heart defects research
Harvard University
2019-2025
Henan Academy of Sciences
2025
University of Rochester
2013-2024
Shaanxi University of Technology
2007-2023
Inspire
2020
University of Rochester Medical Center
2016
Baidu (China)
2013
State Planning Organization
2006
Mechanical breathing motions have a fundamental function in lung development and disease, but little is known about how they contribute to host innate immunity. Here we use human alveolus chip that experiences cyclic breathing-like deformations investigate whether physical forces influence immune responses viral infection. Influenza H3N2 infection of mechanically active chips induces cascade including increased permeability, apoptosis, cell regeneration, cytokines production, recruitment...
The rising threat of pandemic viruses, such as SARS-CoV-2, requires development new preclinical discovery platforms that can more rapidly identify therapeutics are active in vitro and also translate vivo . Here we show human organ-on-a-chip (Organ Chip) microfluidic culture devices lined by highly differentiated primary lung airway epithelium endothelium be used to model virus entry, replication, strain-dependent virulence, host cytokine production, recruitment circulating immune cells...
Human-to-human transmission of viruses, such as influenza viruses and coronaviruses, can promote virus evolution the emergence new strains with increased potential for creating pandemics. Clinical studies analyzing how a particular type progressively evolves traits, resistance to antiviral therapies, result passing between different human hosts are difficult carry out because complexity, scale, cost challenge. Here, we demonstrate that spontaneous A through both mutation gene reassortment be...
The orientation and dispersion of nanoparticles can greatly influence the conductivity mechanical properties nanocomposites. In this study, Polypropylene/ Carbon Nanotubes (PP/CNTs) nanocomposites were produced using three different molding methods, i.e., compression (CM), conventional injection (IM), interval (IntM). Various CNTs content shear conditions give states. Then, electrical percolation thresholds (4 wt.% CM, 6 IM, 9 IntM) obtained by various orientations. Agglomerate (Adis),...
Lung manifestations of chronic obstructive pulmonary disease (COPD) are often exacerbated by influenza A virus infections; however, the underlying mechanisms remain largely unknown and hence, therapeutic options limited. Using a physiologically relevant human lung airway-on-a-chip (Airway Chip) microfluidic culture model lined with airway epithelium from COPD or healthy donors interfaced microvascular endothelium, we observed that Airway Chips exhibit an increased sensitivity to infection,...
Cystic fibrosis (CF) is a genetic disease caused by mutations in the CF transmembrane conductance regulator ( CFTR ) gene, leading to chronic, unresolved inflammation of airways due uncontrolled recruitment polymorphonuclear leukocytes (PMNs). Evidence indicates that loss‐of‐function, addition promoting pro‐inflammatory phenotype, associated with an increased risk developing cancer, suggesting can exert tumor‐suppressor functions. Three‐dimensional (3D) vitro culture models, such as lung...
Lung manifestations of chronic obstructive pulmonary disease (COPD) are often exacerbated by influenza A virus infections; however, the underlying mechanisms remain largely unknown, and hence therapeutic options limited. Using a physiologically relevant human lung airway-on-a-chip (Airway Chip) microfluidic culture model lined with airway epithelium from COPD or healthy donors interfaced microvascular endothelium, we observed that Airway Chips exhibit an increased sensitivity to infection,...
The current coronavirus disease 2019 (COVID-19) pandemic highlights the need for broad-spectrum antiviral therapeutics. Here we describe a new class of self-assembling immunostimulatory short duplex RNAs that potently induce production type I and III interferon (IFN-I IFN-III). These require minimum 20 base pairs, lack any sequence or structural characteristics known RNAs, instead unique motif (sense strand, 5'-C; antisense 3'-GGG) mediates end-to-end dimer self-assembly. presence terminal...
Abstract Here we demonstrate that influenza virus replication, host responses to infection, evolution through mutation or gene reassortment, and clinical efficacy of antiviral drugs can be reconstituted in a human Airway Chip microfluidic culture device. Modeling human-to-human transmission infection the continued presence on chips led emergence clinically prevalent mutations responsible for amantadine- oseltamivir-resistance, as well discovery new resistance mutations. Analysis resulted...
Aiming at the difficulty of temperature control in laser cladding process high-temperature nickel-based alloys, influence parameters on molten pool, and quality layer were explored. Firstly, through analysis finite element method, Inconel718 single-pass model was established surface 45 steel by using parametric language life–death technology, different power scanning speed pool center explored, reasonable scope selected. Secondly, taking as independent variables, dilution rate forming...
Abstract Multivalent presentation of ligands often enhances receptor activation and downstream signaling. DNA origami offers precise nanoscale spacing ligands, a potentially useful feature for therapeutic nanoparticles. Here we introduce “square block” platform to explore the importance CpG oligonucleotides, which engage Toll-like receptors thereby act as danger signals dendritic cells. Through in vitro cell-culture studies vivo tumor-treatment models, demonstrate that square blocks induce...
Disease models that can accurately recapitulate human pathophysiology during infection and clinical response to antiviral therapeutics are still lacking, which represents a major barrier in drug development. The emergence of Organs-on-a-Chip integrated microfluidics with three-dimensional (3D) cell culture, may become the potential solution for this urgent need. Human aims by incorporating tissue-relevant types their microenvironment, such as dynamic fluid flow, mechanical cues,...
Abstract Alveolar macrophages (AMs) are the major sentinel immune cells in human alveoli and play a central role eliciting host inflammatory responses upon distal lung viral infection. Here, we incorporated peripheral monocyte-derived within microfluidic Lung Alveolus Chip that recreates alveolar-capillary interface under an air-liquid along with vascular flow to study how residential AMs contribute pulmonary response When Chips were cultured infected influenza H3N2, there was reduction...
An intact lung epithelial barrier is essential for homeostasis. The Na+, K+-ATPase (NKA), primarily serving as an ion transporter, also regulates function via modulation of tight junctions. However, the underlying mechanism not well understood. Here, we show that overexpression NKA β1 subunit upregulates expression junction proteins, leading to increased alveolar by transport–independent mechanism. Using IP and mass spectrometry, identified a number unknown protein interactions subunit,...