A5102999810- Muscle Physiology and Disorders
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Neonatal Respiratory Health Research
- Microbial Inactivation Methods
- Immune Response and Inflammation
- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Inflammasome and immune disorders
- Pulmonary Hypertension Research and Treatments
- Virus-based gene therapy research
- Hydrogen's biological and therapeutic effects
- CRISPR and Genetic Engineering
- Respiratory Support and Mechanisms
- Animal Nutrition and Physiology
- Caveolin-1 and cellular processes
- Radiopharmaceutical Chemistry and Applications
- Antioxidants, Aging, Portulaca oleracea
- HER2/EGFR in Cancer Research
- Cardiac Arrest and Resuscitation
- Aquaculture Nutrition and Growth
- Ferroptosis and cancer prognosis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Soochow University
2021-2025
First People's Hospital of Kunshan
2021-2025
Qilu University of Technology
2025
Anhui Polytechnic University
2025
Jiangsu University
2021-2025
Duke University Hospital
2021-2024
Duke Medical Center
2021-2024
University of Rochester
2010-2024
Southern Medical University
2024
Nanfang Hospital
2024
Abstract Over the last two decades, lipid nanoparticles (LNPs) have evolved as an effective biocompatible and biodegradable RNA delivery platform in fields of nanomedicine, biotechnology, drug delivery. They are novel bionanomaterials that can be used to encapsulate a wide range biomolecules, such mRNA, demonstrated by current successes COVID‐19 mRNA vaccines. Therefore, it is important provide perspective on LNPs for delivery, which further offers useful guidance researchers who want work...
Acute respiratory distress syndrome (ARDS) afflicts 200,000 patients annually with a mortality rate of 30% to 60% despite wide use low tidal volume (LTV) ventilation, the present standard care. High-permeability alveolar edema and instability occur early in development ARDS, before clinical signs lung injury, represent potential targets for therapy. We hypothesize that application protective ventilation strategy (airway pressure release [APRV]) will stabilize alveoli reduce edema, preventing...
After 480 days of age, high-producing hens are likely to be subject ovarian aging, mainly due oxidative stress. In this study, the amelioration aging in chickens, using a plant antioxidant, lycopene, was investigated. The activity Nrf2/HO-1 pathway chicken ovaries at different ages (90, 150, 280 and 580 old) were compared elucidate any age-related changes. Subsequently, putative attenuating effect lycopene (100 ng/mL) on evaluated through establishment D-gal-induced culture model. cultured...
Oxidative stress is an important inducement in ovarian aging which results fecundity decline human and diverse animals. As a potent antioxidant, grape seed proanthocyanidin extract (GSPE) was investigated to ameliorate chicken this study. Firstly, antioxidant capacity of hens at different ages (90, 150, 280, 580 days old) compared elucidate its age-related changes. Subsequently, D-gal-induced (2.5 mg/mL) model established the cultured tissues were treated with GSPE 5 μ g/mL for 72 h evaluate...
Pulmonary fibrosis is a chronic, progressive lung disease associated with damage and scarring. The pathological mechanism causing pulmonary remains unknown. Emerging evidence suggests prominent roles of epithelial-mesenchymal transition (EMT) alveolar epithelial cells (AECs) in myofibroblast formation fibrosis. Our previous work has demonstrated the regulation YY1 idiopathic pathogenesis fibroid lung. However, specific function AECs during yet to be determined. Herein, we found higher level...
Background Human rhinoviruses (HRVs) are a highly prevalent cause of acute respiratory infection in children. They classified into at least three species, HRV-A, HRV-B and HRV-C, which characterized by sequencing the 5′ untranslated region (UTR) or VP4/VP2 genome. Given increased interest for novel HRV strain identification their worldwide distribution, we have carried out clinical molecular diagnosis strains 2-year study children with visiting one district hospital Shanghai....
Established acute respiratory distress syndrome (ARDS) is often refractory to treatment. Clinical trials have demonstrated modest treatment effects, and mortality remains high. Ventilator strategies must be developed prevent ARDS.Early ventilatory intervention will block progression ARDS if the ventilator mode (1) maintains alveolar stability (2) reduces pulmonary edema formation.Yorkshire pigs (38-45 kg) were anesthetized subjected a "two-hit" ischemia-reperfusion peritoneal sepsis. After...
Objective Osteoarthritis (OA) is a common disease with complex and unclear pathogenesis. Ferroptosis new cell death mode, which proved to be involved in different kinds of disease. We hypothesized that ferroptosis contributes the progress human OA. Design Chondrocytes were extracted from waste cartilage total knee arthroplasty, stimulated interleukin-1β (IL-1β). Then, we detected morphology, proliferation, viability, levels Fe 3+ , glutathione (GSH), reactive oxygen species (ROS),...
Chronic limb-threatening ischemia (CLTI), a severe manifestation of peripheral arterial disease (PAD), is associated with 1-year limb amputation rate approximately 15-20% and substantial mortality. A key feature CLTI the compromised regenerative ability skeletal muscle; however, mechanisms responsible for this impairment are not yet fully understood. In study, we aim to delineate pathological changes at both cellular transcriptomic levels, as well in cell-cell signaling pathways, muscle...
Abstract In developing B cells, V(D)J recombination assembles exons encoding IgH and Igκ variable regions from hundreds of gene segments clustered across Igh Igk loci. V, D J are flanked by conserved signal sequences (RSSs) that target RAG endonuclease 1 . orchestrates upon capturing a H -RSS within the -RSS-based centre 1–3 (RC). orientation programmes to scan upstream D- V -containing chromatin is presented in linear manner cohesin-mediated loop extrusion 4–7 During scanning, robustly...
The integrity and normal function of the small intestinal epithelium depends critically on rapid renewal epithelial cells from basal stem cells. intensive proliferation that fuels this self-renewal process is confined to crypts. Establishment suitable protocols for crypt isolation culture pivotal studies mechanisms. In study, chicken crypts were isolated, purified, further cultured in a Matrigel 3-D system. growth factor concentration assay fourth d showed Group C (50 ng/mL epidermal (EGF),...
Resistance to chemotherapy is the main reason for treatment failure and poor prognosis in patients with triple-negative breast cancer (TNBC). Although association of RNA N6-methyladenosine (m6A) modifications therapy resistance noticed, its role development therapeutic TNBC not well documented. This study aimed investigate potential mechanisms underlying reactive oxygen species (ROS) regulation doxorubicin (DOX)-resistant TNBC. Here, we found that DOX-resistant cells displayed low ROS levels...
Purpose: Osteoarthritis (OA) is a common degenerative disease, which still lacks specific therapeutic drugs. Synovitis one of the most important pathological process in OA. Therefore, we aim to identify and analyze hub genes their related networks OA synovium with bioinformatics tools provide theoretical basis for potential Materials methods: Two datasets were obtained from GEO. DEGs synovial tissue screened through Gene Ontology (GO) annotation, Kyoto Encyclopedia Genes Genomes (KEGG)...
In this study we examined the effects of hydroxyethyl starch (HES 200/0.5) on lung capillary permeability in endotoxic rats and explored possible mechanisms. Male Wistar were randomly divided into seven groups treated with saline, lipopolysaccharide (LPS; 6 mg/kg), LPS plus HES (3.75, 7.5, 15, or 30 mL/kg), (30 mL/kg) alone for 4 2 h. Lung permeability, neutrophil accumulation, expression CD11b blood cell surface, cytokine-induced chemoattractant protein level, nuclear factor kappa B...
A balance between self-renewal and differentiation is critical for the regenerative capacity of tissue-resident stem cells. In skeletal muscle, successful regeneration requires orchestrated activation, proliferation, muscle satellite cells (MuSCs) that are normally quiescent. subset MuSCs undergoes to replenish cell pool, but features identify define self-renewing remain be elucidated. Here, through single-cell chromatin accessibility analysis, we reveal versus trajectories over course in...
In response to the relentless mutation of coronavirus disease, current artificial intelligence algorithms for automated diagnosis COVID-19 via CT imaging exhibit suboptimal accuracy and efficiency. This manuscript proposes a multi-objective optimization algorithm (MOAOA) enhance BiLSTM model diagnosis. The proposed approach involves configuring several hyperparameters bidirectional long short-term memory (BiLSTM), optimized using MOAOA intelligent algorithm, subsequently validated on...
The differentiation of fibroblasts into myofibroblasts is a cardinal feature idiopathic pulmonary fibrosis (IPF). transcription factor Yin Yang 1 (YY1) plays role in the proliferation and diverse cell types, but its fibrotic lung diseases not known.