Objective Osteoarthritis (OA) is a common disease with complex and unclear pathogenesis. Ferroptosis new cell death mode, which proved to be involved in different kinds of disease. We hypothesized that ferroptosis contributes the progress human OA. Design Chondrocytes were extracted from waste cartilage total knee arthroplasty, stimulated interleukin-1β (IL-1β). Then, we detected morphology, proliferation, viability, levels Fe 3+ , glutathione (GSH), reactive oxygen species (ROS),...

Purpose: Osteoarthritis (OA) is a common degenerative disease, which still lacks specific therapeutic drugs. Synovitis one of the most important pathological process in OA. Therefore, we aim to identify and analyze hub genes their related networks OA synovium with bioinformatics tools provide theoretical basis for potential Materials methods: Two datasets were obtained from GEO. DEGs synovial tissue screened through Gene Ontology (GO) annotation, Kyoto Encyclopedia Genes Genomes (KEGG)...

Heart development requires robust gene regulation, and the related disruption could lead to congenital heart disease (CHD). To gain insights into regulation of expression in CHD, we obtained profiles long non-coding RNAs (lncRNAs) messenger (mRNAs) 22 tissue samples with tetralogy Fallot (TOF) through strand-specific transcriptomic analysis. Using a causal inference framework based on correlations validated microRNA (miRNA)–lncRNA–mRNA evidences, constructed competing endogenous RNA...

ChatGPT represents a groundbreaking AI application that has garnered significant attention since its inception. However, despite promising potential, ethical implications have sparked considerable debate. This study aims to examine the key concerns surrounding governance of by conducting bibliometric analysis and cluster-based content relevant scientific literature. The identifies influential authors, countries, pivotal publications, revealing three primary categories issues associated with...

Abstract Chronic psoriasis is a kind of immune-mediated skin illness and the underlying molecular mechanisms pathogenesis remain incompletely understood. Here, we used small RNA microarray assays to scan differential expressed RNAs in patient samples. The downstream miRNAs its targets were predicted using bioinformatics analysis from online bases confirmed fluorescence situ hybridization dual‑luciferase report gene assay. Cell ability proliferation migration detected CCK-8 transwell assays....

Objective— The transcription factor early growth response (EGR)-1 has been implicated as a key vascular phenotypic switch through its control of inducible transcription. EGR-1 autoregulation, and histone modification in the promoter, represent mechanisms control, but have not explored. Methods Results— We demonstrate that regulates own this involves H3 phosphorylation acetylation. transactivates promoter smooth muscle cells exposed to interleukin (IL) 1β novel cis -acting element...

Background/Aims: Interferon regulatory factor 1 (IRF-1) has been shown to function as a transcriptional activator or repressor of variety target genes. However, its upstream, non-coding RNA-related capacity remains unknown. In this study, we focus on the miRNA-associated single nucleotide polymorphisms (SNPs) in 3′untranslated region (UTR) IRF-1 further investigate functional relationship and potential diagnostic value SNPs miRNAs among Chinese gastric cancer (GC) patients. Methods: We...

Mounting evidence suggests that long noncoding RNAs serve as specific biomarkers and potent modulators of multiple cancers. Long intergenic non-protein coding RNA 324 (LINC00324) is ubiquitously expressed in various tissues, including breast cancer. The biological function LINC00324 the development progression cancer remains unknown. Here, we fully elucidate relation between expression cancer, suggest a potential mechanism action. We found decreased was dramatically correlated with...

Human gastric cancer is a serious disease with high mortality rate all over the world. The one of difficulties in effective therapy metastasis. It has been reported that lncRNAs and miRNAs are involved So, exploration new molecular mechanism underlying metastasis involving network lncRNAs/miRNAs/effector proteins important meaningful for guiding treatment cancer.MTT assay, flow cytometric analysis colony formation assay were performed to evaluate AGS or MKN-45 cell proliferation, cycle...

Abstract Background 20-hydroxyecdysone (20E) plays important roles in insect molting and metamorphosis. 20E-induced autophagy has been detected during the larval–pupal transition different insects. In Bombyx mori , is induced by 20E larval fat body. Long non-coding RNAs (lncRNAs) function various biological processes many organisms, including Many lncRNAs have reported to be potential for occurrence mammals, but it not investigated Results RNA libraries from body of B. dissected at 2 6 h...

Neohesperidin (NH) was reported to regulate osteoclastic differentiation, while LncRNA SNHG1 could inhibit osteogenic differentiation of bone marrow stromal cells (BMSCs). In this study, we aimed explore whether SNHG1-mediated be regulated by NH. Osteonecrosis and adjacent tissues, as well normal samples were gathered. BMSCs isolated from Ficoll density gradient identified flow cytometry. Histopathological changes tissues detected hematoxylin-eosin staining. After the treatment with NH or...

Abstract Background Osteoporosis (OP) is a bone disease characterized by reduced amount and quality of bone. This study was designed to explore the role mechanism lncRNA IGF2‐AS in osteogenic differentiation marrow mesenchymal stem cells (BMSCs). Methods Human miRNA microarray analyses were performed measure differential expression levels lncRNAs miRNAs undifferentiated osteogenically differentiated BMSCs. IGF2‐AS, miR‐3,126‐5p, KLK4 measured real‐time quantitative polymerase chain reaction...

Background Osteosarcoma is a common bone sarcoma that occurs in childhood and adolescence. Although research on non-coding RNAs (ncRNAs) of osteosarcoma has been developed rapidly recent years, specific bibliometric analysis this topic not yet performed. The aims to summarize knowledge atlas, hotspots, emerging trends provide researchers with new perspectives further studies. Methods All publications regarding ncRNAs published from 2000 2021 were retrieved the Web Science Core Collection....

Thromboangiitis obliterans (TAO) is a non-atherosclerotic, segmental, chronic vascular inflammatory disease. Our aim was to explore the underlying mechanisms of long non-coding RNA (lncRNA)-related competing endogenous RNAs (ceRNAs) in TAO. Six blood samples were collected from patients with TAO and healthy individuals (three for each category). Total extracted participant sequenced. Differentially expressed lncRNAs (DE-lncRNAs) miRNAs (DE-miRNAs) screened, ceRNA networks associated...

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Abstract Non-coding RNAs (ncRNAs) have long been considered the "white elephant" on genome because they lack ability to encode proteins. However, in recent years, more and biological experiments clinical reports proved that ncRNAs account for a large proportion organisms. At same time, play decisive role processes such as gene expression cell growth development. Recently, it has found short sequence non-coding RNA(miRNA) RNA(lncRNA) can regulate each other, which plays an important various...

Abstract Background : 20-hydroxyecdysone (20E) plays important roles in insect molting and metamorphosis. 20E-induced autophagy has been detected during the larval–pupal transition different insects. In Bombyx mori, is induced by 20E larval fat body. Long non-coding RNAs (lncRNAs) function various biological processes many organisms, including Many lncRNAs have reported to be potential for occurrence mammals, but it not investigated Results RNA libraries from body of B. mori dissected at 2 6...

Abstract This study aimed to investigate the therapeutic efficacy of programmed spatial anatomy myopectineal orifice technique in laparoscopic total extraperitoneal hernioplasty (TEP) surgery. A 121 adult male patients with unilateral inguinal hernias who underwent TEP Department General Surgery, Wujin Hospital, affiliated Jiangsu University, from January 2019 December 2020 were selected. Patients divided into procedural (63 cases) and traditional groups (58 according surgical methods...

<p>Supplementary Data</p>