A5008581924- Machine Learning in Bioinformatics
- Neuroendocrine Tumor Research Advances
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Lung Cancer Research Studies
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- vaccines and immunoinformatics approaches
- Ion channel regulation and function
- Genomics and Phylogenetic Studies
- Cardiac electrophysiology and arrhythmias
- Neuroblastoma Research and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Peptidase Inhibition and Analysis
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Congenital heart defects research
- Fungal Infections and Studies
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Medical Imaging and Pathology Studies
- interferon and immune responses
- Gait Recognition and Analysis
Second Xiangya Hospital of Central South University
2023-2025
Central South University
2023-2025
Soochow University
2025
First Affiliated Hospital of Soochow University
2025
The University of Texas Health Science Center at Houston
2017-2024
Xiangya Hospital Central South University
2024
Chongqing Normal University
2024
Wuhan University of Technology
2024
Huazhong Agricultural University
2024
Wake Forest University
2024
Abstract In eukaryotes, protein phosphorylation is specifically catalyzed by numerous kinases (PKs), faithfully orchestrates various biological processes, and reversibly determines cellular dynamics plasticity. Here we report an updated algorithm of Group-based Prediction System (GPS) 5.0 to improve the performance for predicting kinase-specific sites (p-sites). Two novel methods, position weight determination (PWD) scoring matrix optimization (SMO), were developed. Compared with other...
Abstract Various posttranslational modifications (PTMs) participate in nearly all aspects of biological processes by regulating protein functions, and aberrant states PTMs are frequently implicated human diseases. Therefore, an integral resource PTM–disease associations (PDAs) would be a great help for both academic research clinical use. In this work, we reported PTMD, well-curated database containing that associated with We manually collected 1950 known PDAs 749 proteins 23 types 275...
In the experiments here, time- and voltage-dependent properties of Ca2+-independent, depolarization-activated K+ currents in adult mouse ventricular myocytes were characterized detail. majority (65 72, ≈ 90%) cells dispersed from ventricles, analysis decay phases outward revealed three distinct current components: a rapidly inactivating, transient current, Ito,f (mean ± SEM τdecay = 85 2 ms); slowly 1,162 29 ms) inactivating IK,slow; non steady state Iss. small subset (7 10%) cells, was...
Cyclic nucleotide phosphodiesterases (PDEs) through the degradation of cGMP play critical roles in maintaining cardiomyocyte homeostasis. Ca(2+)/calmodulin (CaM)-activated cGMP-hydrolyzing PDE1 family may a pivotal role balancing intracellular Ca(2+)/CaM and signaling; however, its function cardiomyocytes is unknown.Herein, we investigate Ca(2+)/CaM-stimulated regulating pathological hypertrophy neonatal adult rat ventricular myocytes heart vivo.Inhibition activity using PDE1-selective...
Lung injury, whether induced by infection or caustic chemicals, initiates a series of complex wound-healing responses. If uncontrolled, these responses may lead to fibrotic lung diseases and loss function. Thus, resolution injury must be tightly regulated. The key regulatory proteins required for controlling the have yet identified. Here we show that deubiquitinase CYLD led development fibrosis in mice after with Streptococcus pneumoniae. inhibited transforming growth factor-β-signalling...
DNA N4-methylcytosine (4mC) modification represents a novel epigenetic regulation. It involves in various cellular processes, including replication, cell cycle and gene expression, among others. In addition to experimental identification of 4mC sites, silico prediction sites the genome has emerged as an alternative promising approach. this study, we first reviewed current progress computational systematically evaluated predictive capacity eight conventional machine learning algorithms well...
Abstract —An in vivo experimental strategy, involving cardiac-specific expression of a mutant Kv 2.1 subunit that functions as dominant negative, was exploited studies focused on exploring the role members Kv2 subfamily pore-forming (α) subunits generation functional voltage-gated K + channels mammalian heart. A Kv2.1 α (Kv2.1N216) designed to produce truncated protein containing intracellular N terminus, S1 membrane–spanning domain, and portion S1/S2 loop. The Kv2.1N216 epitope tagged at C...
Previous studies have demonstrated a role for voltage-gated K + (Kv) channel α subunits of the Kv4 subfamily in generation rapidly inactivating/recovering cardiac transient outward current, I to,f , channels. Biochemical suggest that mouse ventricular channels reflect heteromeric assembly Kv4.2 and Kv4.3 with accessory subunits, KChIP2 Kvβ1, is primary determinant regional differences (mouse ventricular) densities. Interestingly, phenotypic consequences manipulating expression different...
cAMP plays crucial roles in cardiac remodeling and the progression of heart failure. Recently, we found that expression hydrolyzing phosphodiesterase 3A (PDE3A) was significantly reduced human failing hearts, accompanied by up-regulation inducible early repressor (ICER) expression. Angiotensin II (Ang II) β-adrenergic receptor agonist isoproterenol (ISO) also induced persistent PDE3A down-regulation concomitant ICER vitro , which is important Ang II- ISO-induced cardiomyocyte apoptosis. We...
Background— Diabetes mellitus is a major risk factor for cardiovascular mortality by increasing endothelial cell (EC) dysfunction and subsequently accelerating atherosclerosis. Extracellular-signal regulated kinase 5 (ERK5) activated steady laminar flow regulates EC function nitric oxide synthase expression inhibiting inflammation. However, the role regulatory mechanisms of ERK5 in atherosclerosis are poorly understood. Here, we report critical p90 ribosomal S6 (p90RSK)/ERK5 complex diabetes...
Abstract As one of the most important reversible types post-translational modification, protein methylation catalyzed by methyltransferases carries many pivotal biological functions as well essential processes. Identification sites is prerequisite for decoding regulatory networks in living cells and understanding their physiological roles. Experimental methods are limitations labor-intensive time-consuming. While silicon approaches cost-effective high-throughput manner to predict potential...
Mutations in kinases are abundant and critical to study signaling pathways regulatory roles human disease, especially cancer. Somatic mutations kinase genes can affect drug treatment, both sensitivity resistance, clinically used inhibitors. Here, we present a newly constructed database, KinaseMD (kinase response), structurally functionally annotate mutations. integrates 679 374 somatic mutations, 251 522 network-rewiring events, 390 460 response records curated from various sources for 547...
Abstract Chemoresistance is one of the major causes poor prognosis in osteosarcoma. Alternative therapeutic strategies for osteosarcoma are limited, indicating that increasing sensitivity to currently used chemotherapies could be an effective approach improve patient outcomes. Using a kinome-wide CRISPR screen, we identified PRKDC as critical determinant doxorubicin (DOX) The analysis clinical samples demonstrated was hyperactivated osteosarcoma, and functional experiments showed loss...
Non-typeable Haemophilus influenza (NTHi) is an important human pathogen causing respiratory tract infections in both adults and children. NTHi are characterized by inflammation, which mainly mediated nuclear transcription factor kappaB (NF-κB)-dependent production of inflammatory mediators. The deubiquitinating enzyme cylindromatosis (CYLD), loss was originally reported to cause a benign syndrome called cylindromatosis, has been identified as key negative regulator for NF-κB vitro. However,...
Abstract Summary: Lysine succinylation orchestrates a variety of biological processes. Annotation in proteomes is the first-crucial step to decipher physiological roles implicated pathological In this work, we developed novel site online prediction tool, called SuccFind, which constructed predict lysine sites based on two major categories characteristics: sequence-derived features and evolutionary-derived information sequence via an enhanced feature strategy for further optimizations. The...
Abstract Motivation Protein malonylation is a novel post-translational modification (PTM) which orchestrates variety of biological processes. Annotation in proteomics the first-crucial step to decipher its physiological roles are implicated pathological Comparing with expensive and laborious experimental research, computational prediction can provide an accurate effective approach identification many types PTMs sites. However, there still no online predictor for lysine malonylation. Results...
Abstract Motivation DNA N6-methyladenine (6 mA) has recently been found as an essential epigenetic modification, playing its roles in a variety of cellular processes. The abnormal status 6 mA modification reported cancer and other disease. annotation marks genome is the first crucial step to explore underlying molecular mechanisms including regulatory roles. Results We present novel online site tool, mA-Finder, by incorporating seven sequence-derived information three physicochemical-based...
Introduction: We report a case of relapse and refractory acute B lymphoblastic leukemia (r/r B-ALL) apparent turned to myeloid (AML), which is occasional fatal during CAR-T treatment. There are still limited data clarify the molecular mechanism blast populations proliferation Case Presentation: A 21-year-old man with an established history r/r B-ALL complex chromosome karyotype renal extramedullary infiltration presented our institution. He exhibited rapid monocytic 19 days after CD19 cell...