A5103094931- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Cardiomyopathy and Myosin Studies
- Atherosclerosis and Cardiovascular Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Muscle Physiology and Disorders
- Cancer, Hypoxia, and Metabolism
- Protein Tyrosine Phosphatases
- Protease and Inhibitor Mechanisms
- Telomeres, Telomerase, and Senescence
- Spaceflight effects on biology
- Protein Kinase Regulation and GTPase Signaling
- Kruppel-like factors research
- Erythrocyte Function and Pathophysiology
- Genetics, Aging, and Longevity in Model Organisms
- Nitric Oxide and Endothelin Effects
- Phagocytosis and Immune Regulation
- Nuclear Receptors and Signaling
- Hippo pathway signaling and YAP/TAZ
- Immune cells in cancer
- Cardiovascular Health and Disease Prevention
- interferon and immune responses
The University of Texas MD Anderson Cancer Center
2013-2023
Osaka Rosai Hospital
2023
National Coalition of Independent Scholars
2023
Scripps MD Anderson Cancer Center
2020
Fujitsu (China)
2009-2018
Baylor College of Medicine
2017
Shandong University
2017
University of Rochester
2006-2015
Cardiovascular Institute Hospital
1999-2015
University of Manchester
2012-2014
The interrelationships of the endoplasmic reticulum (ER), microtubules, and intermediate filaments were studied in peripheral regions thin, spread fibroblasts, epithelial, vascular endothelial cells culture. We combined a fluorescent dye staining technique to localize ER with immunofluorescence microtubules or same cell. Microtubules are sparse lamellipodia, but usually completely absent. These relationships indicate that advance into lamellipodia before filaments. observed tubules have...
When microtubules are fixed in glutaraldehyde the presence of tannic acid and thin sections cut, subunit structure microtubule is readily observed without need image reinforcement. Seven types were analyzed: those heliozoan axoneme, mitotic apparatus, contractile axostyle, repolymerized derived from chick brain, central pair flagella, A tubules flagella basal body. In all cases composed 13 equally spaced protofilaments. The B body appear to be 11 subunits. connections C described. model a presented.
Antibodies against actin were used to corroborate the presence of as a major component protein isolated brain postsynaptic densities. The same antibodies also an immunohistochemical stain study distribution in sections intact tissue. This showed two sites where is concentrated: smooth muscle cells around blood vessels and sites. In area highest concentration occurs densities there intense staining surrounding cytoplasm, especially within dendritic spines. Antiactin was much weaker other...
Metabolic stress in obesity induces endothelial inflammation and activation, which initiates adipose tissue inflammation, insulin resistance, cardiovascular diseases. However, the mechanisms underlying induction are not completely understood. Stimulator of interferon genes (STING) is an important molecule immunity inflammation. In present study, we sought to determine role STING palmitic acid-induced activation/inflammation.
Background: Sporadic aortic aneurysm and dissection (AAD), caused by progressive smooth muscle cell (SMC) loss extracellular matrix degradation, is a highly lethal condition. Identifying mechanisms that drive degeneration crucial step in developing an effective pharmacologic treatment to prevent disease progression. Recent evidence has indicated cytosolic DNA abnormal activation of the sensing adaptor STING (stimulator interferon genes) play critical role vascular inflammation destruction....
It is widely accepted that actin filaments and the conventional double-headed myosin interact to generate force for many types of nonmuscle cell motility, this interaction occurs when regulatory light chain (MLC) phosphorylated by MLC kinase (MLCK) together with calmodulin Ca2+. However, recent studies indicate Rho-kinase also involved in regulating smooth muscle contractility. We have recently isolated reactivatable stress fibers from cultured cells established them as a model system...
Fluid shear stress (FSS) induces many forms of responses, including phosphorylation extracellular signal–regulated kinase (ERK) in endothelial cells (ECs). We have earlier reported rapid tyrosine platelet cell adhesion molecule-1 (PECAM-1) ECs exposed to FSS. Osmotic changes also induced similar PECAM-1 and ERK with nearly identical kinetics. Because both FSS osmotic should mechanically perturb the membrane, they might activate same mechanosignaling cascade. When is phosphorylated by or...
Terminal webs prepared from mouse intestinal epithelial cells were examined by the quick-freeze, deep-etch, and rotary-replication method. The microvilli of these contain actin filaments that extend into terminal web in compact bundles. Within bundles remain compact; few are separated fewer still bend towards lateral margins cell. Decoration with subfragment 1 (S1) myosin confirmed relatively travel horizontally web. Instead, between there complicated networks fibrils. Here we present two...
To understand the roles of Rho-kinase and myosin light chain kinase (MLCK) for contraction organization stress fibers, we treated cultured human foreskin fibroblasts with several MLCK, Rho-kinase, or calmodulin inhibitors analyzed F-actin in cells. Some cells were transfected green fluorescent protein (GFP)-labeled actin, effects also studied these living The Y-27632 HA1077 caused disassembly fibers focal adhesions central portion cell within 1 h. However, located periphery not severely...
The organization of actin and myosin in vascular endothelial cells situ was studied by immunofluorescence microscopy. Examination perfusion-fixed, whole mounts normal mouse rat descending thoracic aorta revealed the presence axially oriented stress fibers containing both within cells. In species, proportion varied from region to same vessel. Some mesenteric vein inferior vena cava also contained fibers. Quantitative studies age-matched normotensive spontaneously hypertensive rats significant...
Stress fibers were isolated from cultured human foreskin fibroblasts and bovine endothelial cells, their contraction was demonstrated in vitro. Cells culture dishes first treated with a low-ionic-strength extraction solution then further extracted using detergents. With gentle washes by pipetting, the nucleus apical part of cells removed. The material on dish scraped, freed forced through hypodermic needle fractionated sucrose gradient centrifugation. Isolated, free-floating stress stained...
Stress fiber-like patterns are visualized by indirect immunofluorescence in scleroblasts (fibroblasts) situ on the scale of common goldfish, Carassius auratus, using an affinity-purified antiactin, antimyosin, and anti-alpha-actinin. These fibers demonstrate classical convergent parallel exhibited stress tissue culture cells. Because dimensions, composition, pattern distribution these cytoplasmic correspond well with those cultured cells, we will call also. The staining anti-alpha-actinin...
The capping of surface Ig on B cells occurs with a striking redistribution cytoplasmic myosin. Our results suggest close association between and myosin which could be the basis for stimulated motility.
We have studied the distribution of myosin and tubulin molecules inside same tissue culture cells by using two antibodies labeled with contrasting fluorochromes. Antimyosin raised against human platelet was rhodamine. Antitubulin sea urchin vinblastine-induced crystals fluorescein. The stained entirely different structures flat interphase cell: antimyosin bound to stress fibers antitubulin thin, wavy thought be individual microtubules. Compact diffusely both antibodies. From prophase through...
Immunofluorescence microscopy was used to determine the number of endothelial cells with stress fibers for three age groups, and distinct anatomical locations within descending thoracic aorta both normotensive spontaneously hypertensive rats. For each group examined, rats consistently demonstrated greater fiber expression than did Neither nor blood pressure predominant influence on in aortic endothelium. In rats, remained unchanged all groups examined. strains, however, more were found those...