A5056647947- Enzyme Production and Characterization
- Atherosclerosis and Cardiovascular Diseases
- Microbial Metabolites in Food Biotechnology
- Ubiquitin and proteasome pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Melanoma and MAPK Pathways
- Protein Kinase Regulation and GTPase Signaling
- Chemotherapy-induced cardiotoxicity and mitigation
- Angiogenesis and VEGF in Cancer
- Phytase and its Applications
- Biofuel production and bioconversion
- Cancer, Hypoxia, and Metabolism
- Nitric Oxide and Endothelin Effects
- Telomeres, Telomerase, and Senescence
- Receptor Mechanisms and Signaling
- Cardiac Imaging and Diagnostics
- Protein Tyrosine Phosphatases
- Signaling Pathways in Disease
- Immune cells in cancer
- Food composition and properties
- Phosphodiesterase function and regulation
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
The University of Texas MD Anderson Cancer Center
2016-2025
Kagoshima University
2001-2024
The University of Texas Health Science Center at Houston
2023
NTT (Japan)
2023
Palo Alto Institute
2021
Texas A&M Health Science Center
2018-2021
Scripps MD Anderson Cancer Center
2020
Hokkaido University
1987-2018
Baylor College of Medicine
2016-2017
Shandong University
2017
Metabolic stress in obesity induces endothelial inflammation and activation, which initiates adipose tissue inflammation, insulin resistance, cardiovascular diseases. However, the mechanisms underlying induction are not completely understood. Stimulator of interferon genes (STING) is an important molecule immunity inflammation. In present study, we sought to determine role STING palmitic acid-induced activation/inflammation.
Background: Sporadic aortic aneurysm and dissection (AAD), caused by progressive smooth muscle cell (SMC) loss extracellular matrix degradation, is a highly lethal condition. Identifying mechanisms that drive degeneration crucial step in developing an effective pharmacologic treatment to prevent disease progression. Recent evidence has indicated cytosolic DNA abnormal activation of the sensing adaptor STING (stimulator interferon genes) play critical role vascular inflammation destruction....
Abstract RNA velocity provides an approach for inferring cellular state transitions from single-cell sequencing (scRNA-seq) data. Conventional models infer universal kinetics all cells in scRNA-seq experiment, resulting unpredictable performance experiments with multi-stage and/or multi-lineage transition of cell states where the assumption same kinetic rates no longer holds. Here we present cellDancer, a scalable deep neural network that locally infers each its neighbors and then relays...
Big mitogen-activated kinase 1 (BMK1) or extracellular signal-regulated kinase-5 (ERK5) has recently been identified as a new member of the protein family. We have shown that BMK1 is activated to greater extent by H<sub>2</sub>O<sub>2</sub> than growth factors, suggesting in comparison with other family members, redox-sensitive kinase. Previous investigations indicate tyrosine c-Src mediates signal transduction reactive oxygen species, including H<sub>2</sub>O<sub>2</sub>. Therefore, role...
c-Jun NH2-terminal kinase (JNK) is activated by a number of cellular stimuli such as inflammatory cytokines and environmental stresses. Reactive oxygen species also cause activation JNK; however, the signaling cascade that leads to JNK remains be elucidated. Because recent reports showed expression Cas, putative Src substrate, stimulates activation, we hypothesized family Cas would involved in reactive species. An essential role for both was demonstrated. First, specific tyrosine inhibitor,...
Inflammation is a hallmark of many diseases, such as atherosclerosis, chronic obstructive pulmonary disease, arthritis, infectious and cancer. Although steroids cyclooxygenase inhibitors are effective antiinflammatory therapeutical agents, they may cause serious side effects. Therefore, developing unique agents without significant adverse effects urgently needed. Vinpocetine, derivative the alkaloid vincamine, has long been used for cerebrovascular disorders cognitive impairment. Its role in...
Reactive oxygen species (ROS) activate Ras and the extracellular signal-regulated kinase (ERK) cascade. Because JAK2 is a critical mediator for Ras/Raf/ERK activation by several hormones, we examined role of in ROS signal events. H2O2 stimulated activity fibroblasts with peak at 2–5 min. To determine specific Src Fyn as mediators its downstream events, used derived from transgenic mice deficient (Src−/−) or (Fyn−/−). H2O2-stimulated was completely inhibited Fyn−/− cells. Shc tyrosine...
Myocyte apoptosis plays an important role in pathological cardiac remodeling and the progression of heart failure. cAMP signaling is crucial regulation myocyte remodeling. Multiple cAMP-hydrolyzing phosphodiesterases (PDEs), such as PDE3 PDE4, coexist cardiomyocytes elicit differential temporal/spatial signaling. However, PDE4 cardiomyocyte remains unclear. Although chronic treatment with inhibitors increases mortality patients failure, contribution expression/activity failure not well...
Oxidative stress, generated by excessive reactive oxygen species, promotes cardiovascular disease. Cyclophilin A (CyPA) is a 20-kDa chaperone protein secreted from vascular smooth muscle cells (VSMCs) in response to species that stimulates VSMC proliferation and inflammatory cell migration vitro; however, the role CyPA plays function vivo remains unknown.We tested hypothesis contributes remodeling analyzing complete carotid ligation knockout mice, wild-type mice overexpress (VSMC-Tg). After...
Cyclophilin A (CyPA; encoded by Ppia) is a ubiquitously expressed protein secreted in response to inflammatory stimuli. CyPA stimulates vascular smooth muscle cell migration and proliferation, endothelial adhesion molecule expression, chemotaxis. Given these activities, we hypothesized that would promote atherosclerosis. Apolipoprotein E–deficient (Apoe−/−) mice fed high-cholesterol diet for 16 wk developed more severe atherosclerosis compared with Apoe−/−Ppia−/− mice. Moreover, deficiency...
Cyclic nucleotide phosphodiesterases (PDEs) through the degradation of cGMP play critical roles in maintaining cardiomyocyte homeostasis. Ca(2+)/calmodulin (CaM)-activated cGMP-hydrolyzing PDE1 family may a pivotal role balancing intracellular Ca(2+)/CaM and signaling; however, its function cardiomyocytes is unknown.Herein, we investigate Ca(2+)/CaM-stimulated regulating pathological hypertrophy neonatal adult rat ventricular myocytes heart vivo.Inhibition activity using PDE1-selective...
Background— Diabetes mellitus is a major risk factor for cardiovascular mortality by increasing endothelial cell (EC) dysfunction and subsequently accelerating atherosclerosis. Extracellular-signal regulated kinase 5 (ERK5) activated steady laminar flow regulates EC function nitric oxide synthase expression inhibiting inflammation. However, the role regulatory mechanisms of ERK5 in atherosclerosis are poorly understood. Here, we report critical p90 ribosomal S6 (p90RSK)/ERK5 complex diabetes...
Insulin-like growth factor (IGF)-1 and the type I IGF-1 receptor are important regulators of vascular function that may contribute to cardiovascular disease. We hypothesized causes endothelial cell dysfunction expression neutrophil monocyte adhesion molecules by enhancing pro-inflammatory cytokine signal transduction. Long-term treatment cells potentiated c-Jun nuclear NF-κB activation tumor necrosis (TNF)-α enhanced TNF-α–mediated molecule expression. In response treatment, kinases in...
The mitogen-activated protein kinases (MAPKs) play important roles in regulation of cell growth and survival. Human MAPK 5 (ERK5) or Big MAP kinase 1 (BMK1) is a recently cloned member the family. To identify ERK5-related kinases, we searched GenBank™ expressed sequence tag (EST) data base for mouse cDNAs with homology to human ERK5. A full-length cDNA that was highly homologous ERK5 identified. Further analysis polymerase chain reaction products generated from embryo yielded three (mERK5a,...