A5084994614- Bacterial biofilms and quorum sensing
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Inflammasome and immune disorders
- Peptidase Inhibition and Analysis
- Antimicrobial Peptides and Activities
- Vibrio bacteria research studies
- Heat shock proteins research
- interferon and immune responses
- Cancer Mechanisms and Therapy
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Streptococcal Infections and Treatments
- Amoebic Infections and Treatments
- Cell Image Analysis Techniques
- Biofuel production and bioconversion
- Galectins and Cancer Biology
- Pharmaceutical and Antibiotic Environmental Impacts
- Pneumonia and Respiratory Infections
- Digital Holography and Microscopy
- Viral Infectious Diseases and Gene Expression in Insects
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
Korea University
2015-2025
Yeungnam University
2014
University of Rochester Medical Center
2006-2008
House Clinic
2006-2007
University of Southern California
2006-2007
University of Florida
2000-2005
University of Arkansas for Medical Sciences
1999-2000
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that primarily infects immunocompromised individuals and patients with cystic fibrosis. Using a tissue culture system, invasive strains of P. were discovered to induce apoptosis at high frequency in HeLa other epithelial fibroblast cell lines. This apoptotic phenotype the infected cells was determined by several criteria including (i) visual changes morphology, (ii) induction chromatin condensation nuclear marginalization, (iii)...
DsbA is a periplasmic thiol:disulfide oxidoreductase which contributes to the process of protein folding by catalyzing formation disulfide bonds. In this study, we demonstrate that dsbA gene required for expression type III secretion system under low-calcium inducing conditions, intracellular survival P. aeruginosa upon infection HeLa cells, and twitching motility. The diverse phenotypes mutant are likely due its defect in proteins involved various biological processes, such as signal...
Non-typeable Haemophilus influenza (NTHi) is an important human pathogen causing respiratory tract infections in both adults and children. NTHi are characterized by inflammation, which mainly mediated nuclear transcription factor kappaB (NF-κB)-dependent production of inflammatory mediators. The deubiquitinating enzyme cylindromatosis (CYLD), loss was originally reported to cause a benign syndrome called cylindromatosis, has been identified as key negative regulator for NF-κB vitro. However,...
Abstract Epithelial cells represent the first line of host innate defense against invading microbes by elaborating a range molecules involved in pathogen clearance. In particular, epithelial mucins facilitate mucociliary clearance physically trapping inhaled microbes. Up-regulation mucin production thus represents an important response How is induced upper respiratory Streptococcus pneumoniae infections unknown. this study, we show that pneumolysin required for up-regulation MUC5AC via...
We have previously reported on the isolation of in vivo inducible genes Pseudomonas aeruginosa using IVET system. One such isolated from burn mouse infection model encodes a short open reading frame with unknown function. In this study, we demonstrate that gene product specifically suppresses expression type III secretion P. aeruginosa, thus named PtrA (Pseudomonas repressor A). A direct interaction between and transcriptional activator ExsA was demonstrated, suggesting its function is...
Gram-positive bacterium Streptococcus pneumoniae is an important human pathogen that colonizes the upper respiratory tract and also major cause of morbidity mortality worldwide. S. causes invasive diseases such as pneumonia, meningitis, otitis media. Despite importance pneumococcal diseases, little known about molecular mechanisms by which pneumoniae-induced inflammation regulated, especially negative regulatory mechanisms. Here we show activates nuclear factor activated T cells (NFAT)...
Recently, a number of microcontaminants were found in aquatic environment and it raises the concerns about their effects to ecosystem. This study investigated if low levels antibiotics can trigger biofilm formation result enhanced antibiotic gene transfer. For this purpose, enteric bacteria (Escherichia coli), environmental micro-organism (Pseudomonas aeruginosa), mixture by ppb level investigated. In addition, on conjugation E. coli with P. aeruginosa structure also evaluated using...
Pseudomonas aeruginosa is a Gram-negative opportunistic human pathogen possessing type III secretion system (T3SS) which injects toxic effector proteins into mammalian host cells. In previous studies, P. strains lacking all of the known effectors were shown to cause cytotoxicity upon prolonged infection time. this study, we report identification new cytotoxin, nucleoside diphosphate kinase (NDK), injected eukaryotic cells in T3SS-dependent manner. Injection NDK inhibited by presence...
Membrane impermeability is the major contributing factor to multidrug resistance in clinical isolates of Pseudomonas aeruginosa. By using laboratory strain PAK, a spontaneous P. aeruginosa mutant (mutant PAK1-3) whose membrane had reduced permeability and which displayed increased levels various antibiotics, especially aminoglycosides, was isolated. complementation with genomic clone library derived from wild-type novel two-component regulatory system (PprA PprB) identified found be able...
The type III secretion system of Pseudomonas aeruginosa is tightly regulated by various environmental signals, such as low calcium and contact with the host cell. However, exact signals triggering are unknown. present study describes finding that P. effector molecules requires protein factors from serum L broth, designated (TSFs), in addition to low-calcium environment. In absence TSF or chelator EGTA, basal levels accumulated intracellularly. Addition EGTA together effectively triggers...
ABSTRACT Clinical isolates of Pseudomonas aeruginosa are classified into invasive and noninvasive (cytolytic) strains. In a PA103 background, ExoS ExoT have recently been shown to function as anti-internalization factors. However, these two factors seemed not such in an strain PAK background. this study, using HeLa tissue culture cells, we observed that the internalization is dependent on its growth phases, with stationary-phase cells internalized about 100-fold more efficiently than...
The transforming growth factor β (TGF-β) pathway represents an important signaling involved in the regulation of diverse biological processes, including cell proliferation, differentiation, and apoptosis. Despite known role TGF-βR-mediated suppressing immune response, its regulating human Toll-like receptors (TLRs), key host defense that recognize invading bacterial pathogens, however, remains unknown. Here, we show for first time TGF-βR-Smad3/4 acts as a positive regulator TLR2 induction by...
Bacteraemic pneumonia is a common cause of sepsis in critically ill patients today and characterized by dysregulation inflammation. The genetic factors predisposing to bacteraemic are not yet fully understood. Innate immunity pivotal for host defence against invading bacteria, nuclear factor-kappa B (NF-kappaB) central bacteria-induced inflammation immune responses. deubiquitinating enzyme CYLD has been identified as key negative regulator NF-kappaB. In the present study, we investigated...
Mucosal epithelial cells in the respiratory tract act as first line of host innate defense against inhaled microbes by producing a range molecules for clearance. In particular, mucins facilitate mucociliary clearance physically trapping microbes. Up-regulation mucin production thus represents an important response invading Excess production, however, overwhelms clearance, resulting defective mucosal defenses. Thus, tight regulation is critical maintaining appropriate balance between...
As one of the first lines host defense, monocytes play important roles in clearing infected microbes. The defensive response is triggered by recognition diverse microbial moieties, including released factors, which modulate immune responses to establish a harsh environment for clinically bacterial pathogens. In this study, we found that expression PTX3, soluble form pattern receptor, was induced infection with live Pseudomonas aeruginosa or treatment cells its supernatant. P. GroEL, homolog...
ABSTRACT Burn wounds are prone to infection by Pseudomonas aeruginosa , which is an opportunistic pathogen causing various human diseases. During infection, the bacterium senses environmental changes and regulates expression of genes appropriate for survival. A purine-auxotrophic mutant P. was unable replicate efficiently on burn wounds, suggesting that purine-deficient environments. An in vivo technology based purEK gene applied burned mouse model isolate specifically induced during...
Endometriosis, defined as the presence of endometrial cells outside uterine cavity, is a major cause infertility and pelvic pain, afflicting more than 10% reproductive age women. Endometriosis chronic inflammatory disease lipopolysaccharide promotes proliferation invasion endometriotic stromal cells. Cysteine-rich secretory protein LCCL domain-containing 2 (CRISPLD2) has high affinity for plays critical role in defense against endotoxin shock. However, function CRISPLD2 not been studied...
Toll-like receptor 2 (TLR2) plays a critical role in mediating inflammatory/immune responses against bacterial pathogens lung. Streptococcus pneumoniae (S. pneumoniae) and nontypeable Haemophilus influenzae (NTHi) were previously reported to synergize with each other induce inflammatory responses. Despite the relatively known intracellular signaling pathways involved synergistic induction of inflammation, it is still unclear if both also synergistically expression surface TLR2. Here we...
Type III-secreted effectors are essential for modulating host immune responses during the pathogenesis of Pseudomonas aeruginosa infections. Little is known about impact one effectors, ExoY, on inflammasome activation, which results in IL-1β production and pyroptotic cell death. In this study, we found that transcriptional expression Il-1β was induced to a lesser extent response an exoY-harboring strain than deleted mutant. This suppressive effect ExoY verified by complementation assay as...