A5063022953- Mechanisms of cancer metastasis
- Immune Response and Inflammation
- Immune cells in cancer
- S100 Proteins and Annexins
- Cancer Mechanisms and Therapy
- Bioactive Natural Diterpenoids Research
- Neutropenia and Cancer Infections
- Nitrogen and Sulfur Effects on Brassica
- Cancer, Hypoxia, and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Blood disorders and treatments
- Cytokine Signaling Pathways and Interactions
- Peptidase Inhibition and Analysis
- Cell death mechanisms and regulation
- Immune Cell Function and Interaction
- Pharmacological Effects of Natural Compounds
- Phagocytosis and Immune Regulation
- NF-κB Signaling Pathways
- Inflammation biomarkers and pathways
- Oral health in cancer treatment
- Cell Adhesion Molecules Research
- Nanoplatforms for cancer theranostics
- Phytochemistry and Biological Activities
- Enzyme Structure and Function
Korea Research Institute of Bioscience and Biotechnology
2015-2024
Korea University of Science and Technology
2012-2020
CHA Bundang Medical Center
2011-2014
CHA University
2009-2014
Genome Research Foundation
2012
Genomics Medicine (Ireland)
2010
Plant (United States)
2009
Systems Engineering Research Center
2009
Chungnam National University
2009
Stem Cell Institute
2009
Abstract We searched for potential suppressors of tumor metastasis by identifying the genes that are frequently down-regulated in hepatocellular carcinomas (HCC) while being negatively correlated with clinical parameters relevant to metastasis, and we report here on identification N-myc downstream regulated gene 2 (NDRG2) as a promising candidate. NDRG2 expression was significantly reduced HCC compared nontumor or normal liver tissues [87.5% (35 40) 62% (62 100) at RNA protein levels,...
Downregulation of the N-myc downstream-regulated gene 2 (NDRG2) is involved in progression aggressive forms cancer, along with poor prognosis cancer patients. In current study, we examined effect NDRG2 expression on metastatic potential HT1080 human fibrosarcoma and B16F10 murine melanoma cells both vitro vivo systems. gelatin zymography, remarkably suppressed matrix metalloproteinase (MMP)-9 activity slightly inhibited MMP-2 cell lines. Tumor migration invasion were significantly reduced by...
Summary A new proinflammatory cytokine interleukin‐32 (IL‐32) has six isoforms. Although IL‐32 can be detected in sera from patients suffering Crohn’s disease and rheumatoid arthritis, it is unclear which isoforms are involved. To this end, we investigated the functions of most abundant IL‐32β by generating K562‐IL‐32β stable cell lines. This report confirms, using small interfering RNA, that induces an anti‐inflammatory IL‐10 cells U937 promonocytic cells, express endogenous upon phorbol...
Abstract Recently, the anti‐tumor activity of N‐myc downstream‐regulated gene 2 (NDRG2) was elucidated, but molecular mechanism how NDRG2 works as a tumor suppressor is not well known. To determine function suppressor, we established stable cell lines expressing protein or its mutant forms, and studied their effects on growth. Interestingly, constitutive expression wild‐type induced growth retardation SW620 colon carcinoma cells. Introduction into cells decrease c‐Jun phosphorylation at...
Although N-myc downstream regulated gene 2 (NDRG2) has been known to be a tumor suppressor gene, the function of this not elucidated. In present study, we investigated expression and NDRG2 in human gastric cancer. Among seven cancer two non-cancer cell lines, only SNU-16 SNU-620, expressed NDRG2, which was detected cytoplasm. Interestingly, highly normal tissues, but patients were divided into NDRG2-positive -negative groups. The survival rate NDRG2-negative lower than that patients. We...
NDRG (N-Myc downstream-regulated gene)-2 is a member of the family. Although it has been suggested that NDRG2 involved in cellular differentiation and tumor suppression, its intracellular signal regulatory mechanism are not well known. Here, we show differential expression human colon carcinoma cell lines tissues by reverse transcription–polymerase chain reaction immunohistochemical analyses with monoclonal antibody against NDRG2. was strongly expressed normal colonic mucosa adenomatous (25...
Considerable attention has recently been paid to the N-Myc downstream-regulated gene (NDRG) family because of its potential as a tumor suppressor in many human cancers. Primary amino acid sequence information suggests that NDRG proteins may belong α/β-hydrolase (ABH) superfamily; however, their functional role not yet determined. Here, we present crystal structures and mouse NDRG2 determined at 2.0 1.7 Å resolution, respectively. Both show remarkable structural similarity ABH superfamily,...
We searched for genes with expressions specific to human monocyte‐derived dendritic cells (DCs) using differential display reverse transcription‐polymerase chain reaction, and found that N‐myc downstream regulated gene 2 (NDRG2), a member of new family differentiation‐related genes, was expressed in DCs. While DCs derived from CD34 + progenitor also showed strong NDRG2 expression, the corresponding mRNA expression absent other cell lines including monocytes, B cells, NK cells. The inhibition...
Abstract BACKGROUND: Kallikrein‐related peptidase 6 (KLK6) encodes a trypsin‐like serine protease that is up‐regulated in several cancers, although the putative functions of KLK6 cancer have not been elucidated. In current study, overexpression was identified colon cancer, and possibility may be suitable candidate as tumor marker examined. METHODS: Messenger RNA (mRNA) transcript levels protein up‐regulation tissues examined using reverse transcriptase‐polymerase chain reaction,...
We have previously reported on the isolation of in vivo inducible genes Pseudomonas aeruginosa using IVET system. One such isolated from burn mouse infection model encodes a short open reading frame with unknown function. In this study, we demonstrate that gene product specifically suppresses expression type III secretion P. aeruginosa, thus named PtrA (Pseudomonas repressor A). A direct interaction between and transcriptional activator ExsA was demonstrated, suggesting its function is...
Chemotherapy-induced mucositis can limit the effectiveness of cancer therapy and increase risk infections. However, no specific for protection against is currently available. In this study, we investigated therapeutic effect PLAG (1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol, acetylated diglyceride) in 5-fluorouracil (5-FU)-induced oral animal models. Hamsters were administered 5-FU (80 mg/kg) intraperitoneally on days 0, 6, 9. The animals' cheek pouches then scratched equally with tip an...
// Sang Yoon Park 1, 8 , Seon-Jin Lee 2 Hee Jun Cho 1 Tae Woo Kim Jong-Tae Jae Wha 3 Chul-Ho 4 Bo-Yeon 5 Young Il Yeom 6 Jong-Seok Lim 7 Younghee Gu Genome Structure Research Center, Korea Institute of Biosceience and Biotechnology, Daejeon, Department Biomolecular Science, University Science Technology (UST), Biomedical Translational Animal Resource World Class Institute, Bioscience Cheongju, Functional Genomics, Biological Sciences, The Center for Women's Diseases, Sookmyung University,...
The type III secretion system of Pseudomonas aeruginosa is tightly regulated by various environmental signals, such as low calcium and contact with the host cell. However, exact signals triggering are unknown. present study describes finding that P. effector molecules requires protein factors from serum L broth, designated (TSFs), in addition to low-calcium environment. In absence TSF or chelator EGTA, basal levels accumulated intracellularly. Addition EGTA together effectively triggers...
Mac-2 binding protein (Mac-2BP) is a secreted tumor antigen that elevated in many cancers and implicated metastasis, as well cell adhesion immune functions. We focused on the human telomerase reverse transcriptase (hTERT) induced Mac-2BP expression relationship between progression of gastric cancer. A cDNA array analysis was performed telomerase-negative line, SW13, which engineered to overexpress hTERT when compared with parental SW13 cell. hTERT-induced confirmed via RT-PCR Northern...
The type III secretion system (T3SS) of Pseudomonas aeruginosa plays a significant role in pathogenesis. We have previously identified factor (TSF), which is required for effective the effector molecules, addition to low calcium signal. TSF includes many low-affinity high-capacity binding proteins, such as serum albumin and casein. A search targets on bacterial outer membrane resulted identification PopN, component T3SS that readily detectable cell surface. PopN specifically interacts with...