A5017770296- Lipid metabolism and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Antifungal resistance and susceptibility
- Biofuel production and bioconversion
- Soybean genetics and cultivation
- Fungal Infections and Studies
- Peroxisome Proliferator-Activated Receptors
- Fungal and yeast genetics research
- Parasitic Infections and Diagnostics
- Peptidase Inhibition and Analysis
- Pneumocystis jirovecii pneumonia detection and treatment
- Trace Elements in Health
- Enzyme Catalysis and Immobilization
- Metabolomics and Mass Spectrometry Studies
- Polyamine Metabolism and Applications
- Fatty Acid Research and Health
- Enzyme Production and Characterization
- Adipose Tissue and Metabolism
- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Chromium effects and bioremediation
- Algal biology and biofuel production
- Biopolymer Synthesis and Applications
- Bacterial biofilms and quorum sensing
- Microbial Metabolites in Food Biotechnology
Xi'an University of Science and Technology
2024
Nankai University
2007-2016
In the human fungal pathogen Candida albicans, environmental pH has profound effects on morphogenesis and response to extracellular is clearly relevant pathogenicity of this fungus. Yeast cells have evolved a complex network mechanisms in they often require integration Rim101 calcineurin/Crz1 signaling pathways. Ca(2+) burst common cellular when are exposed stresses; therefore, study, we asked whether it follows same case under alkaline stress calcium change regulated by Rim101p Crz1p. We...
Abstract The pre-identification of impulse road surfaces, such as speed bumps, potholes, and manhole covers, can significantly enhance the performance active suspension systems. However, existing current identification methods often fail to balance between low cost, high reliability, precise speed. This study proposes an surface method based on a reinforcement learning network. First, real dataset surfaces was collected, with dynamic responses serving inputs random levels along features...
The maintenance of cellular calcium homeostasis is associated with signalling transduction and the functions many membrane compartments, especially endoplasmic reticulum (ER) function. ER-localized proteins that serve to maintain ER in Candida albicans are still unclear. In this study, Spf1, putative C. homologue Saccharomyces cerevisiae P-type ATPase ScSpf1, was investigated for its roles homeostasis, hyphal development virulence. We constructed an Spf1 null mutant which showed decreased...
Candida albicans morphogenesis and gastrointestinal colonization are closely associated with the pathogenicity of this pathogen. This study investigated in vitro vivo effect verapamil, a calcium channel blocker, on these processes. Exposure to ≥ 10 μg mL(-1) verapamil led significant decrease C. hyphal cells. The ability adhere polystyrene surface buccal epithelial cells was inhibited by exposure 20 verapamil. Detection Hwp1-green fluorescent protein fusion showed that expression transport...
Iron is an essential element required for most organisms. The high-affinity iron-uptake systems in the opportunistic pathogen Candida albicans are activated under iron-limited conditions and also virulence. Here one component of systems, multicopper oxidase (MCO) genes, was characterized. We examined expression five MCO genes demonstrated that CaFET3 CaFET34 were major response to iron deficiency. Complementation Saccharomyces cerevisiae fet3Δ mutant showed could effectively rescue growth...
A cDNA sequence putatively encoding a Delta(6)-fatty acid desaturase was isolated from Rhizopus arrhizus using reverse transcription polymerase chain reaction and rapid amplification of ends methods. Sequence analysis indicated that this had an open reading frame 1377 bp 458 amino acids 52 kDa. The deduced showed high similarity to those fungal desaturases which comprised the characteristics membrane-bound desaturases, including three conserved histidine-rich motifs hydropathy profile....
The type III secretion system (T3SS) of Pseudomonas aeruginosa plays a significant role in pathogenesis. We have previously identified factor (TSF), which is required for effective the effector molecules, addition to low calcium signal. TSF includes many low-affinity high-capacity binding proteins, such as serum albumin and casein. A search targets on bacterial outer membrane resulted identification PopN, component T3SS that readily detectable cell surface. PopN specifically interacts with...
Candida albicans is an important opportunistic pathogen, causing both superficial mucosal infections and life-threatening systemic diseases. Iron acquisition factor for pathogen–host interaction also a significant element the pathogenicity of this organism. Ferric reductases, which convert ferric iron into ferrous iron, are components high-affinity uptake system. Sequence analyses have identified at least 17 putative reductase genes in C. genome. CFL1 was first albicans. However, little...
ABSTRACT. A cDNA sequence was cloned from the filamentous fungus Thamnidium elegans As3.2806 using reverse transcription polymerase chain reaction (RT‐PCR) and rapid amplification of ends method (RACE). Sequence analysis indicated that this has an open reading frame 1,380 bp, which encodes a 52.4 kDa peptide 459 amino acids. The designated acid high similarity with found in fungal delta 6‐fatty desaturases: it shows three conserved histidine‐rich motifs two hydrophobic domains. cytochrome b...
Iron is an essential nutrient required for the growth and metabolism in Candida albicans. Here, we first time identified Mrs4 as a new member of mitochondrial carrier family C. Our experiments revealed that albicans (CaMrs4) localized to mitochondria morphology. We found CaMrs4 cell growth, mrs4Δ/Δ mutant showed more severe defect iron deficiency. Deletion MRS4 affected cellular content by altering expression regulon genes albicans, such AFT2, SMF3, FTR1 ISU1. Aft2 factor functions negative...
Morphological transition and iron metabolism are closely relevant to Candida albicans pathogenicity virulence. In our previous study, we demonstrated that C. Aft2 plays an important role in ferric reductase activity Here, further explored the roles of numerous cellular processes. We found exhibited through bi-directional regulation effects on iron-regulon expression. Deletion AFT2 reduced accumulation under iron-deficient conditions. Furthermore, both reactive oxygen species (ROS) generation...
Based on the sequence information of Δ 12 ‐fatty acid desaturase genes (from Mucor circinelloides , Mortierella alpina rouxii and Aspergillus nidulans ), which were involved in conversion from C18:1 to C18:2, a cDNA putatively encoding was isolated Rhizopus arrhizus using combination reverse transcription‐polymerase chain reaction (RT‐PCR) rapid amplification ends (RACE) methods. Sequence analysis indicated that it had an open reading frame (ORF) 1170 bp, coding for 389 amino residues 45...