A5091389949- Reproductive tract infections research
- Cervical Cancer and HPV Research
- Pelvic floor disorders treatments
- Reproductive System and Pregnancy
- Urinary Tract Infections Management
- Aquaculture disease management and microbiota
- Physiological and biochemical adaptations
- Heat shock proteins research
- Immune Response and Inflammation
- Insect and Arachnid Ecology and Behavior
- Neonatal Respiratory Health Research
- Reproductive Biology and Fertility
- Inflammasome and immune disorders
- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Toxin Mechanisms and Immunotoxins
- Immune cells in cancer
- Cancer Research and Treatments
- Vibrio bacteria research studies
- Cancer Cells and Metastasis
- Salmonella and Campylobacter epidemiology
- Respiratory Support and Mechanisms
- Immunotoxicology and immune responses
- Immune Cell Function and Interaction
Harvard University
2022-2025
Inspire
2023-2024
Gates Foundation
2024
Harvard University Press
2024
Indian Institute of Science Education and Research Mohali
2018-2021
Abstract Background A dominance of non-iners Lactobacillus species in the vaginal microbiome is optimal and strongly associated with gynecological obstetric health, while presence diverse obligate or facultative anaerobic bacteria a paucity species, similar to communities found bacterial vaginosis (BV), considered non-optimal adverse health outcomes. Various therapeutic strategies are being explored modulate composition microbiome; however, there no human model that faithfully reproduces...
Abstract Modulation of the cervix by steroid hormones and commensal microbiome play a central role in health female reproductive tract. Here we describe organ-on-a-chip (Organ Chip) models that recreate human cervical epithelial-stromal interface with functional epithelial barrier production mucus biochemical hormone-responsive properties similar to living cervix. When Cervix Chips are populated optimal healthy versus dysbiotic microbial communities (dominated Lactobacillus crispatus...
This study explores the protective role of cervicovaginal mucus in maintaining vaginal health, particularly relation to bacterial vaginosis (BV), using organ chip technology. By integrating human Cervix and Vagina Chips, we demonstrated that cervical significantly reduces inflammation epithelial damage caused by a dysbiotic microbiome commonly associated with BV. Proteomic analysis Chip, following exposure from revealed differentially abundant proteins, suggesting potential biomarkers...
Abstract Alveolar macrophages (AMs) are the major sentinel immune cells in human alveoli and play a central role eliciting host inflammatory responses upon distal lung viral infection. Here, we incorporated peripheral monocyte-derived within microfluidic Lung Alveolus Chip that recreates alveolar-capillary interface under an air-liquid along with vascular flow to study how residential AMs contribute pulmonary response When Chips were cultured infected influenza H3N2, there was reduction...
Vibrio parahaemolyticus is a human pathogen, and it major cause of severe gastroenteritis in coastal areas. OmpU one the outer membrane porins V. Host-immunomodulatory effects (VpOmpU) have not been elucidated yet. In this study, an effort towards characterizing effect VpOmpU on innate immune responses host, we observed that recognized by Toll-like receptor 1/2 (TLR1/2) heterodimer THP-1 monocytes but both TLR1/2 TLR2/6 heterodimers RAW 264.7 macrophages. To best our knowledge, first report...
The ability to tolerate temperature stress is an important component of adult fitness. In holometabolous insects like Drosophila melanogaster, resistance can be affected by growth conditions experienced during the larval stages. Although evolution under crowded known lead correlated many traits, its consequences on heat tolerance have not been investigated. Therefore, in present study, we assessed populations D. melanogaster adapted a stressful crowding environment. We used replicate...
ABSTRACT Modulation of mucus production by the human endo– and ecto-cervical epithelium steroid hormones associated interactions with commensal microbiome play a central role in physiology pathophysiology female reproductive tract. However, most our knowledge about these is based on results from animal studies or vitro models that fail to faithfully mimic mucosal environment cervix. Here we describe microfluidic organ-on-a-chip (Organ Chip) cervical mucosa recreate epithelial-stromal...
Women's health, and particularly diseases of the female reproductive tract (FRT), have not received attention they deserve, even though an unhealthy system may lead to life-threatening diseases, infertility, or adverse outcomes during pregnancy. One barrier in field is that there has been a dearth preclinical, experimental models faithfully mimic physiology pathophysiology FRT. Current vitro animal do fully recapitulate hormonal changes, microaerobic conditions, interactions with vaginal...
Salmonella enterica serovar Typhimurium is known to cause its virulence by secreting various effector proteins directly into the host cytoplasm via two distinct type III secretion systems (T3SS-1 and T3SS-2). Generally, T3SS-1 delivered effectors help S. in early phases of infection including invasion, immune modulation cells, whereas, T3SS-2 mainly survival within cells maintenance Salmonella-containing vacuole (SCV), replication bacteria dissemination. Some are secreted both T3SS-2,...
ABSTRACT Background The cervicovaginal mucus that coats the upper surface of vaginal epithelium is thought to serve as a selective barrier helps clear pathogens, however, its role in modulating physiology and pathophysiology human vagina poorly understood. Bacterial vaginosis (BV), common disease female reproductive tract increases susceptibility sexually transmitted infections, pelvic inflammatory disease, infertility, preterm birth, both maternal neonatal infections characterized by...
ABSTRACT Background A dominance of non-iners Lactobacillus species in the vaginal microbiome is optimal and strongly associated with gynecological obstetric health, while presence diverse obligate or facultative anaerobic bacteria a paucity species, similar to communities found bacterial vaginosis (BV), considered non-optimal adverse health outcomes. Various therapeutic strategies are being explored modulate composition microbiome; however, there no human model that faithfully reproduces...
Abstract Background The cervicovaginal mucus which coats the upper surface of vaginal epithelium is thought to serve as a selective barrier that helps clear pathogens, however, its role in modulating physiology and pathophysiology human vagina poorly understood. Bacterial vaginosis (BV), common disease female reproductive tract increases susceptibility sexually transmitted infections, pelvic inflammatory disease, infertility, preterm birth, both maternal neonatal infections characterized by...
Abstract In mice and one human case study, Borrelia burgdorferi (Bb), the causative agent of Lyme disease (LD), disrupts germinal center architecture function. Bb proteins also mimic self-antigens. Thus, antibody response may be poor quality target self antigens. To understand this pathology its relevance to infection-induced autoimmune diseases, we infected lymphoid organ chips (Goyal et al., Adv Sci., 2022) created from healthy volunteers with Bb. We discovered unexpected severe...