A5024254015- Cardiomyopathy and Myosin Studies
- RNA Research and Splicing
- Muscle Physiology and Disorders
- Asphalt Pavement Performance Evaluation
- Lower Extremity Biomechanics and Pathologies
- Cardiovascular and exercise physiology
- Infrastructure Maintenance and Monitoring
- Sports Performance and Training
- Muscle activation and electromyography studies
- RNA regulation and disease
- Cellular transport and secretion
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Heart Rate Variability and Autonomic Control
- Balance, Gait, and Falls Prevention
- Sports injuries and prevention
- Ion channel regulation and function
- Cytomegalovirus and herpesvirus research
- RNA and protein synthesis mechanisms
- Foot and Ankle Surgery
- Cardiovascular Health and Disease Prevention
- Wnt/β-catenin signaling in development and cancer
- melanin and skin pigmentation
- Genomics and Chromatin Dynamics
- Viral Infections and Immunology Research
Harvard University
2025
McLaughlin Research Institute
2006-2020
Institute for Stem Cell Biology and Regenerative Medicine
2012-2017
National Centre for Biological Sciences
2013
University of Nevada, Las Vegas
2004-2012
Universidade Federal do Estado do Rio de Janeiro
2004
Universidade Castelo Branco
2004
University at Albany, State University of New York
2003
University of Washington Applied Physics Laboratory
2002
University of Oregon
1997-2001
Myosin Va is associated with discrete vesicle populations in a number of cell types, but little known the function myosin Vb. Yeast two-hybrid screening rabbit parietal cDNA library dominant active Rab11a (Rab11aS20V) identified Vb as an interacting protein for Rab11a, marker plasma membrane recycling systems. The isolated clone, corresponding to carboxyl terminal 60 kDa tail, interacted all members Rab11 family (Rab11a, Rab11b, and Rab25). GFP-myosin endogenous immunoreactivity...
Our understanding of the organization postsynaptic signaling systems at excitatory synapses has been aided by identification proteins in density (PSD) fraction, a subcellular fraction enriched structures with morphology PSDs. In this study, we have completed most major PSD use an analytical method based on mass spectrometry coupled searching protein sequence databases. At least one each 26 prominent bands from now identified. We found 7 not previously known to be constituents and 24 that had...
BACKGROUND Evaluation of gene expression in failing human heart has been limited by the availability cardiac tissue. METHODS AND RESULTS We used polymerase chain reaction (PCR) to assess small quantities and nonfailing heart. PCR is a powerful new molecular biological tool that allows quantity DNA be amplified as much 1 million-fold. Total RNA was extracted from 3-5 mg samples reverse-transcribed complementary (cDNA). With selected oligonucleotide primers, we amplify cDNAs encoding atrial...
Mutant alleles at the dilute unconventional myosin heavy chain locus cause diluted coat color, opisthotonic seizures, and death. The color phenotype is caused by irregular clumping of pigment in hair, but amounts melanin are unchanged from wild-type controls. melanocyte has been described as adendritic, since hair bulb Harderian gland melanocytes appear to be rounded tissue sections. These observations do not exclude possibility that processes lack pigment, shape was judged distribution...
The formation of complex subcellular organelles requires the coordinated targeting multiple components. Melanosome biogenesis in mouse melanocytes is an excellent model system for studying function gene products intracellular trafficking. To begin to order events melanosome and distribution, we employed classical coat‐color mutants ashen , dilute leaden which affect but not melanin synthesis. loci have been renamed Rab27a Myo5a Mlph their products. While each three has shown be required...
Spleen cells which replicate murine cytomegalovirus (MCMV) during acute infection in vivo were identified by electron microscopy and combined immunocytochemical staining situ cytohybridization. Most infected cells, as defined hybridization for viral RNA with MCMV-specific probes, shown to be positive factor VIII-related antigen negative Ia, Thy-1, F4/80 antigens. Electron microscopic ultrastructural observations indicated that the spleen are predominantly sinusoidal-lining cells. We also...
Abstract Evi-1 is a putative protooncogene first identified as common site of retroviral integration in murine myeloid leukemias. It encodes 145×103Afr nuclear DNA-binding protein that contains ten zinc-finger motifs separated into two domains, well an acidic domain. These features suggest transcriptional regulatory protein. In Drosophila, proteins such Kruppel are involved body plan patterning, and exhibit spatially restricted pattern expression the embryo. To determine if may be...
Preschool children with attention-deficit/hyperactivity disorder (ADHD) (27 boys, 5 girls, mean age 4 years 10 months) participated in a double-blind placebo-controlled crossover drug study to assess the side effects of methylphenidate. Children received twice daily, for at least 1 week each, placebo, 0.3 mg/kg methylphenidate, and 0.5 Side were monitored by parent rating scale designed medication studies. In general, methylphenidate was tolerated relatively well, no withdrawing because...
We have isolated cDNAs encoding a second member of the dilute (myosin-V) unconventional myosin family in vertebrates, myr 6 (myosin from rat 6). Expression transcripts brain is much more limited than expression dilute, with highest levels observed choroid plexus and components limbic system. mapped locus to mouse chromosome 18 using an interspecific backcross. The 3' portion cDNA sequence nearly identical that previously published putative glutamic acid decarboxylase [Huang, W.M.,...
Vaccines prepared from the envelope glycoprotein, gp120, of common laboratory isolate human immunodeficiency virus type 1 (HIV-1) (IIIB/LAV-1) elicit antibodies that neutralize homologous but show little if any cross-neutralizing activity. This may be because principal neutralizing determinant (PND) gp120 is highly unusual in IIIB/LAV-1 strain and not representative those found majority field isolates. We have now examined immunogenicity recombinant MN HIV-1 (MN-rgp120), whose PND thought to...
Abstract Background Myosin-Vb has been shown to be involved in the recycling of diverse proteins multiple cell types. Studies on transferrin trafficking HeLa cells using a dominant-negative myosin-Vb tail fragment suggested that was required for from perinuclear compartments plasma membrane. However, chemical-genetic, experiments, which specifically induced bind actin, initial hypothesis incorrect both its site and mode action. Instead, chemical-genetic data functions actin-rich periphery as...