A5086375532- Immune Response and Inflammation
- Immune cells in cancer
- Immune Cell Function and Interaction
- Drug-Induced Hepatotoxicity and Protection
- Muscle Physiology and Disorders
- Amyotrophic Lateral Sclerosis Research
- Prion Diseases and Protein Misfolding
- Cardiovascular Effects of Exercise
- Metabolism and Genetic Disorders
- Cardiomyopathy and Myosin Studies
- Clinical Nutrition and Gastroenterology
- Cancer, Hypoxia, and Metabolism
- Neurological diseases and metabolism
- Tryptophan and brain disorders
Wayne State University
2010-2018
Institute for Stem Cell Biology and Regenerative Medicine
2014
Acute liver failure (ALF) can be induced in mice by administering Escherichia coli lipopolysaccharide (LPS) and D-galactosamine (D-GalN), which induce an inflammatory response involving tumour necrosis factor (TNF)-α production a hepatocyte-specific transcriptional block. Under these conditions, binding of TNF-α to its cognate receptor on hepatocytes eventually leads their apoptosis.As part effort identify drugs treat this disease model, we have investigated whether the glutamine synthetase...
The glutamine synthetase inhibitor methionine sulfoximine (MSO), shown previously to prevent death caused by an inflammatory liver response in mice, was tested on vitro production of cytokines mouse peritoneal macrophages triggered with lipopolysaccharide (LPS). MSO significantly reduced the Interleukin 6 (IL-6) and Tumor Necrosis Factor Alpha (TNFα) at 4 h after LPS-treatment. This reduction did not result from decreased transcription IL-6 TNFα genes, therefore appeared post-transcriptional...
Methionine sulfoximine (MSO) is an amino acid that irreversibly inhibits glutamine synthetase (GS). Several studies have linked aberrant GS activity with pathological states, and inhibition by MSO has been extensively reviewed. We previously shown IP injection of (50mg/kg) increases survival decreases pro‐inflammatory cytokine levels in a mouse model acute liver failure. are trying to characterize this activity, we present evidence directly modulates the innate immune response decreasing...