A5062640454- Signaling Pathways in Disease
- Toxin Mechanisms and Immunotoxins
- SARS-CoV-2 and COVID-19 Research
- Myasthenia Gravis and Thymoma
- SARS-CoV-2 detection and testing
- Peptidase Inhibition and Analysis
- Neuroinflammation and Neurodegeneration Mechanisms
- Peripheral Neuropathies and Disorders
- Microfluidic and Bio-sensing Technologies
- Biochemical and Molecular Research
- Autoimmune Neurological Disorders and Treatments
- Long-Term Effects of COVID-19
- Immune cells in cancer
- T-cell and B-cell Immunology
- HIV Research and Treatment
- Cystic Fibrosis Research Advances
- Bacterial Genetics and Biotechnology
- Force Microscopy Techniques and Applications
- Systemic Lupus Erythematosus Research
- Brain Metastases and Treatment
- Antifungal resistance and susceptibility
- RNA regulation and disease
- Retinal Diseases and Treatments
- Immune Cell Function and Interaction
- Food Allergy and Anaphylaxis Research
University of Zurich
2020-2023
Yale University
2018-2022
Baylor University
2022
University of Colorado Anschutz Medical Campus
2022
UCB Pharma (United States)
2022
Agricultural University of Athens
2011-2020
University of Michigan
2020
University of Utah
2020
UCLA Medical Center
2020
Harbor–UCLA Medical Center
2020
Central nervous system (CNS) immune activation is an important driver of neuronal injury during several neurodegenerative and neuroinflammatory diseases. During HIV infection, CNS associated with high rates neurocognitive impairment, even sustained long-term suppressive antiretroviral therapy (ART). However, the cellular subsets that drive damage in infection other neurological conditions remain unknown, part because cells are difficult to access living humans. Using single-cell RNA...
We have profiled cerebrospinal fluid T cells in healthy individuals and patients with MS using single-cell RNA TCR sequencing.
Autoantibodies targeting the acetylcholine receptor (AChR), found in patients with myasthenia gravis (MG), mediate pathology through 3 mechanisms: complement-directed tissue damage, blocking of binding site, and internalization AChR. Clinical assays, used to diagnose monitor patients, measure only autoantibody binding. Consequently, they are limited providing association disease burden, understanding mechanistic heterogeneity, monitoring therapeutic response. The objective this study was...
IgD-CD27- double negative (DN) B cells with proinflammatory characteristics are abnormally elevated in a proportion of multiple sclerosis (MS) patients. In this study, the origin and selection DN were studied MS patients healthy controls (HC). Expression developmental markers on peripheral blood DN, IgD-CD27+ class-switched memory (CSM) IgD+CD27- naive HC (n = 48) 96) was determined by flow cytometry. High-throughput adaptive immune receptor repertoire sequencing performed CSM 3 each). from...
Abstract Effective public-health measures and vaccination campaigns against SARS-CoV-2 require granular knowledge of population-level immune responses. We developed a Tripartite Automated Blood Immunoassay (TRABI) to assess the IgG response ectodomain receptor-binding domain spike protein as well nucleocapsid SARS-CoV-2. used TRABI for continuous seromonitoring hospital patients healthy blood donors (n=72’222) in canton Zurich from December 2019 2020 (pre-vaccine period). Seroprevalence...
The clinical outcome of SARS-CoV-2 infections, which can range from asymptomatic to lethal, is crucially shaped by the concentration antiviral antibodies and their affinity targets. However, polyclonal antibody responses in plasma difficult measure. Here we used microfluidic profiling (MAAP) determine aggregate affinities concentrations anti-SARS-CoV-2 samples 42 seropositive individuals, 19 were healthy donors, 20 displayed mild symptoms, 3 critically ill. We found that dissociation...
Effective public health measures against SARS-CoV-2 require granular knowledge of population-level immune responses. We developed a Tripartite Automated Blood Immunoassay (TRABI) to assess the IgG response three proteins. used TRABI for continuous seromonitoring hospital patients and blood donors (n = 72′250) in canton Zurich from December 2019 2020 (pre-vaccine period). found that antibodies waned with half-life 75 days, whereas cumulative incidence rose 2.3% June 12.2% mid-December 2020. A...
PpiB belongs to the superfamily of peptidyl-prolyl cis/trans isomerases (PPIases, EC: 5.2.1.8), which catalyze rate-limiting protein folding step at bonds and control several biological processes. In this study, we show that acts as a negative effector motility biofilm formation ability Escherichia coli. We identify multicopy suppressors each ΔppiB phenotype among putative prey proteins upon deletion are often characterized by analogous phenotypes. Many preys similar gene expression in...
<h3>Objective</h3> To identify candidate biomarkers associated with neuromyelitis optica spectrum disorder (NMOSD) using high-throughput technologies that broadly assay the concentrations of serum analytes and frequencies immune cell subsets. <h3>Methods</h3> Sera, peripheral blood mononuclear cells (PBMCs), matched clinical data from participants NMOSD healthy controls (HCs) were obtained Collaborative International Research in Clinical Longitudinal Experience Study biorepository. Flow...
Abstract IgE antibodies against the allergen Ara-h2 can cause life-threatening anaphylaxis upon exposure to peanuts. Desensitization strategies aim at inducing IgG responses which may compete with anaphylactogenic IgE. Here we assessed anti-Ara-h2 titers in an unselected cohort of 24,536 adult patients admitted a general hospital for disparate medical reasons. Surprisingly, (n=177) and pediatric (n=76) cystic fibrosis (pwCF) had IgG4, but not IgE, several peanut soybean allergens, yet did...
Escherichia coli PpiB is a peptidyl-prolyl cis/trans isomerase (PPIase, EC: 5.2.1.8) with chaperone activity. Here, we show that the ΔppiB deletion strain and over-expression wild-type are both characterized by defects in cell division involving milder or severe filamentation, respectively. Using various mutants, PPIase activity of necessary for observed whereas other structural features apart from active site also important this phenotype. Early divisome components zipA ftsZ showed...
Abstract The clinical outcome of SARS-CoV-2 infections, which can range from asymptomatic to lethal, is crucially shaped by the concentration antiviral antibodies and their affinity targets. However, polyclonal antibody responses in plasma difficult measure. Here we used Microfluidic Antibody Affinity Profiling (MAAP) determine aggregate affinities concentrations anti-SARS-CoV-2 samples 42 seropositive individuals, 19 were healthy donors, 20 displayed mild symptoms, 3 critically ill. We...
Abstract FklB is a member of the FK506-binding proteins (FKBPs), family that consists five genes in Escherichia coli . Little known about physiological and functional role bacterial movement. In present study, knock-out mutant Δ fklB presented an increased swarming swimming motility biofilm formation phenotype, suggesting negative regulator these cellular processes. Complementation with Peptidyl-prolyl isomerase (PPIase)-deficient gene (Y181A ) revealed defects were not restored by Y181A ,...
Abstract Central nervous system (CNS) immune activation in an important driver of neuronal injury during several neurodegenerative and neuroinflammatory diseases. During HIV infection, CNS is associated with high rates neurocognitive impairment, even sustained long-term suppressive antiretroviral therapy (ART). However, the cellular subsets that drive damage infection neurological conditions remain unknown, part because cells are difficult to access living humans. Using single cell RNA...
Abstract Background Autoantibodies targeting the acetylcholine receptor (AChR), found in patients with myasthenia gravis (MG), mediate pathology through three mechanisms: complement-directed tissue damage, blocking of binding site, and internalization AChR. Clinical assays, used to diagnose monitor patients, measure only autoantibody binding. Consequently, they are limited providing association disease burden, understanding mechanistic heterogeneity, monitoring therapeutic response....