A5021301214- Immune cells in cancer
- Mesenchymal stem cell research
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- Chronic Myeloid Leukemia Treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- Virus-based gene therapy research
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- Tissue Engineering and Regenerative Medicine
- Cancer Immunotherapy and Biomarkers
- Hemoglobinopathies and Related Disorders
- Chronic Lymphocytic Leukemia Research
- Chemokine receptors and signaling
- Angiogenesis and VEGF in Cancer
- CRISPR and Genetic Engineering
- Acute Lymphoblastic Leukemia research
- Pluripotent Stem Cells Research
- Lysosomal Storage Disorders Research
- Circadian rhythm and melatonin
- Phagocytosis and Immune Regulation
- Neonatal Respiratory Health Research
- Nanoparticle-Based Drug Delivery
Instituto Butantan
2022-2024
Universidade de São Paulo
2015-2024
Inserm
2018-2023
Université Paris-Saclay
2020-2023
Clinics Hospital of Ribeirão Preto
2017-2023
Centre National de la Recherche Scientifique
2021-2023
Universidad de Lima
2022
ORCID
2022
Universidade Federal de Minas Gerais
2022
Fondation de l'Avenir
2022
Magnetic hyperthermia (MHT) has been shown as a promising alternative therapy for glioblastoma (GBM) treatment. This study consists of three parts: The first part evaluates the heating potential aminosilane-coated superparamagnetic iron oxide nanoparticles (SPIONa). second and third parts comprise evaluation MHT multiple applications in GBM model, either vitro or vivo. obtained curves SPIONa (100 nm, +20 mV) their specific absorption rates (SAR) stablished best therapeutic conditions...
Survivors from sepsis are in an immunosuppressed state that is associated with higher long-term mortality and risk of opportunistic infections. Whether these factors contribute to neoplastic proliferation, however, remains unclear. Tumor-associated macrophages (TAM) can support malignant cell survival, angiogenesis. We addressed the relationship between post-sepsis state, tumor progression TAM accumulation, phenotypic genetic profile, using a mouse model resolution then B16 melanoma mice. In...
Abstract Introduction Mesenchymal stromal/stem cells (MSCs) are multipotent that have the ability to express and secrete a wide range of immunomodulatory molecules, cytokines, growth factors antiapoptotic proteins. MSCs modulate both innate adaptive immune responses making them potential candidates for treatment patients with type 1 diabetes mellitus (T1D). However, one problem frequently associated systemic administration is entrapment mainly in lungs. In this sense, trying avoid lung...
Abstract Treatment of large bone defects is a challenging clinical situation that may be benefited from cell therapies based on regenerative medicine. This study was conducted to evaluate the effect local injection marrow-derived mesenchymal stromal cells (BM-MSCs) or adipose tissue-derived MSCs (AT-MSCs) regeneration rat calvarial defects. BM-MSCs and AT-MSCs were characterized their expression specific surface markers; viability evaluated after with 21-G needle. Defects measuring 5 mm...
Natural killer 92 (NK-92) cells are an attractive therapeutic approach as alternative chimeric antigen receptor (CAR) carriers, different from T cells, once they can be used in the allogeneic setting. The modest vivo outcomes observed with NK-92 continue to present hurdles successfully translating cell therapies into clinical applications. Adoptive transfer of CAR-NK-92 holds out promise benefit at a lower rate adverse events due absence GvHD and cytokine release syndrome. However, it has...
Diabetes mellitus (DM) is a chronic disease and major cause of mortality morbidity worldwide. The hyperglycemia caused by DM induces micro macrovascular complications that lead, among other consequences, to wounds amputations. Cell therapy tissue engineering constitute recent therapeutic alternatives improve wound healing in diabetic patients. current study aimed analyze the effectiveness biocuratives containing human mesenchymal stem cells (MSCs) associated with hydrogel matrix process...
The circadian clock coordinates biological and physiological functions to day/night cycles. perturbation of the increases cancer risk affects progression. Here, we studied how BMAL1 knockdown (BMAL1-KD) by shRNA epithelial–mesenchymal transition (EMT), a critical early event in invasion metastasis colorectal carcinoma (CRC). In corresponding gene set enrichment analysis, which showed significant EMT invasive signatures BMAL1_high CRC patients as compared BMAL1_low patients, our results...
The endothelial-to-mesenchymal transition (EndMT) is a biological process where endothelial cells (ECs) acquire fibroblastic phenotype after concomitant loss of the apical-basal polarity and intercellular junction proteins. This critical to embryonic development involved in diseases such as fibrosis tumor progression. signaling pathway transforming growth factor β (TGF-β) an important molecular route responsible for EndMT activation. However, it unclear whether anatomic location influences...
The primary objective of this study is to monitor tumor growth by using image techniques and behavioral testing through general specific motor activities (spontaneous movements gait). Our sample includes male Wistar rats, 2 months old weighing 250–300 g, that categorized into three groups: control, sham, experimental. experimental group was anesthetized; the C6 cells with luciferase expression were suspended in a culture medium implanted right frontoparietal cortex rats. sham received...
of 24h.In mammals, the suprachiasmatic nuclei hypothalamus coordinate circadian rhythms via peripheral molecular clocks composed at least fifteen genes that are expressed in every cell.Expression these clock gene is regulated by transcription factors organized positive (BMAL1 and CLOCK) or negative (PER CRY) feedback loops.Briefly, www.aging-us.com
During the last two decades, introduction of tyrosine kinase inhibitors (TKIs) to therapy has changed natural history CML but progression into accelerated and blast phase (AP/BP) occurs in 3-5% cases, especially patients resistant several lines TKIs. In TKI-refractory advanced phases, only curative option is hematopoietic stem cell transplantation. We others have shown relevance expression Interleukin-2-Receptor α subunit (IL2RA/CD25) as a biomarker progression, suggesting its potential use...
Chimeric antigen receptor (CAR) engineering of natural killer (NK) cells have shown promising results in early-phase clinical studies. However, advancing CAR-NK cell therapeutic efficacy is imperative. In this study, we investigated the impact a fourth generation CD19-targeted CAR (CAR.19) co-expressing IL-27 on NK-92 cells. We observed significant improvement proliferation and cytotoxicity activity against B-cell cancer lines, both vitro xenograft mouse lymphoma model. Our systematic...
Abstract Adipose‐derived stromal/stem cells (ASCs) are promising candidates for cell‐based therapies. However, the lack of markers able to unequivocally identify these cells, differential expression cell surface molecules among stromal progenitors from different tissues and cellular alterations caused by culture phenomena that need be comprehensively addressed in order improve ASC purification consequently refine our knowledge about their function therapeutic efficiency. In this study, we...
Aryl Hydrocarbon Receptor (AHR) is an ubiquitous basic helix-loop-helix transcription factor, which ligand-activated and involved in numerous biological processes including cell division, quiescence inflammation. It has been shown that AHR normal hematopoietic progenitor proliferation human cells. In addition, loss of knockout mice accompanied by a myeloproliferative syndrome-like disease, suggesting role stem (HSC) maintenance. To study the potential pathway CML progenitors cells, we have...
Mesenchymal stem/stromal cells (MSC) are promising candidates for cell-based therapies and the promotion of tissue repair, hence increase clinical trials in a worldwide scale. In particular, adipose tissue-derived (AT MSC) present easy accessibility rather straightforward process isolation, providing clear advantage over other sources. The high demand cell doses (millions cells/kg), needed infusion settings, requires scalable efficient manufacturing AT MSC under xenogeneic(xeno)-free culture...