A5047477708- Nanoparticle-Based Drug Delivery
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Glioma Diagnosis and Treatment
- Characterization and Applications of Magnetic Nanoparticles
- Hematopoietic Stem Cell Transplantation
- Advanced MRI Techniques and Applications
- COVID-19 Clinical Research Studies
- Nanoplatforms for cancer theranostics
- Ultrasound and Hyperthermia Applications
- Microfluidic and Bio-sensing Technologies
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Parkinson's Disease Mechanisms and Treatments
- Ginkgo biloba and Cashew Applications
- Hematological disorders and diagnostics
- Metabolomics and Mass Spectrometry Studies
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Neuroscience and Neural Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Gold and Silver Nanoparticles Synthesis and Applications
- Cancer, Lipids, and Metabolism
Hospital Israelita Albert Einstein
2019-2024
Universidade Federal de Pernambuco
2020
Objective: To evaluate the magnetic hyperthermia therapy in glioblastoma tumor-on-a-Chip model using a microfluidics device. Methods: The nanoparticles coated with aminosilane were used for of hyperthermia, being evaluated specific absorption rate at 300 Gauss and 305kHz. A preculture C6 cells was performed before 3D culture on chip. process Chip after administration 20μL (10mgFe/mL) [...]
Magnetic hyperthermia (MHT) has been shown as a promising alternative therapy for glioblastoma (GBM) treatment. This study consists of three parts: The first part evaluates the heating potential aminosilane-coated superparamagnetic iron oxide nanoparticles (SPIONa). second and third parts comprise evaluation MHT multiple applications in GBM model, either vitro or vivo. obtained curves SPIONa (100 nm, +20 mV) their specific absorption rates (SAR) stablished best therapeutic conditions...
Objective: To evaluate the potential of magnetic hyperthermia using aminosilane-coated superparamagnetic iron oxide nanoparticles in glioblastoma tumor model. Methods: The were analyzed as to their stability aqueous medium and heating through specific absorption rate, when submitted with different frequencies intensities alternating field. In vitro assays, C6 cells cultured transduced luciferase by bioluminescence absence/presence [...]
To evaluate the in vitro and vivo toxicities of polyethylene glycol-coated gold nanoparticles synthesized using a one-step process.
This in vitro study aims to evaluate the magnetic hyperthermia (MHT) technique and best strategy for internalization of nanoparticles coated with aminosilane (SPIONAmine) glioblastoma tumor cells. SPIONAmine 50 100 nm were used specific absorption rate (SAR) analysis, performing MHT intensities 50, 150, 300 Gauss frequencies varying between 305 557 kHz. The was performed using 100, 200, µgFe/mL SPIONAmine, or without Poly-L-Lysine (PLL) filter, static dynamic magnet field. cell viability...
Induced pluripotent stem cells (iPSCs) show great ability to differentiate into any tissue, making them attractive candidates for pathophysiological investigations. The rise of organ-on-a-chip technology in the past century has introduced a novel way make vitro cell cultures that more closely resemble their vivo environments, both structural and functionally. literature still lacks consensus on best conditions mimic blood-brain barrier (BBB) drug screening other personalized therapies....
Breast cancer (BC) presents a growing global concern, mainly for the female population of working age. Their pathophysiology shows challenges when attempting to ensure conventional treatment efficacy without adverse effects. This study aimed evaluate magneto-hyperthermia (MHT) therapy associated with supplementation omega-3 polyunsaturated fatty acid (w-3 PUFA) and engagement in physical training (PT) triple-negative BC (TNBC) model. First, we assessed physicochemical properties iron oxide...
This in vitro study aimed to find the best method of granulocyte isolation for subsequentlabeling with multimodal nanoparticles (magnetic and fluorescent properties) enable detectionby optical magnetic resonance imaging (MRI) techniques. The granulocytes were obtained fromvenous blood samples from 12 healthy volunteers. To achieve high purity yield, four differentmethods evaluated. isolated labeled withmultimodal superparamagnetic iron oxide (M-SPIONs) coated dextran, theiron load was...
This study proposes an innovative way to evaluate the homing and tracking of hematopoietic stem cells from young old mice labeled with SPIONNIRF-Rh conjugated two types fluorophores (NIRF Rhodamine), their grafting by bioluminescence (BLI) in a bone marrow transplant (BMT) model. In vitro study, we isolated mononuclear (BM-MNC) mice, analyzed physical–chemical characteristics SPIONNIRF-Rh, internalization, cell viability, iron quantification NIRF, ICP-MS, MRI. The vivo was performed BMT...
Bone marrow transplantation is a treatment for variety of hematological and non-hematological diseases. For the transplant success, it mandatory to have thriving engraftment transplanted cells, which directly depends on their homing. The present study proposes an alternative method evaluate homing hematopoietic stem cells using bioluminescence imaging inductively coupled plasma mass spectrometry (ICP-MS) associated with superparamagnetic iron oxide nanoparticles. We identified enriched...
Abstract Parkinson's disease (PD) induced by environmental toxins involves a multifactorial cascade of harmful factors, thus motivating the search for therapeutic agents able to act on greatest number molecular targets. This study evaluated efficacy 50 mg/kg purified anacardic acids (AAs), isolated from cashew nut shell liquid, multiple steps oxidative stress and inflammation rotenone in substantia nigra (SN) striatum. Adult mice were divided into four groups: Control, rotenone, AAs + alone....