A5053669969- Diabetes and associated disorders
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Salivary Gland Disorders and Functions
- Phytoplasmas and Hemiptera pathogens
- Oral microbiology and periodontitis research
- Plant Pathogenic Bacteria Studies
- RNA and protein synthesis mechanisms
- Hemoglobinopathies and Related Disorders
- Plant Stress Responses and Tolerance
- Immunotherapy and Immune Responses
- Research on Leishmaniasis Studies
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Multiple Sclerosis Research Studies
- SARS-CoV-2 and COVID-19 Research
- Diabetes Management and Research
- Mosquito-borne diseases and control
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Trypanosoma species research and implications
- Force Microscopy Techniques and Applications
- Electromagnetic Fields and Biological Effects
- Insect symbiosis and bacterial influences
- Synthesis and Biological Evaluation
Brazilian Biosciences National Laboratory
2020-2024
Brazilian Center for Research in Energy and Materials
2020-2024
Universidad Americana
2022
Pontifícia Universidade Católica de Campinas
2020
Faculdade de Medicina de Jundiaí
2012-2017
Uninter
2015
Universidade Estadual de Campinas (UNICAMP)
2003-2012
Brazilian Synchrotron Light Laboratory
2003
ABSTRACT Complete sequencing of the Xylella fastidiosa genome revealed characteristics that have not been described previously for a phytopathogen. One characteristic this was abundance genes encoding proteins with adhesion functions related to biofilm formation, an essential step colonization plant host or insect vector. We examined four belonging class encoded by in X. : PilA2 and PilC fimbrial proteins, which are components type IV pili, XadA1 XadA2, afimbrial adhesins. Polyclonal...
Molecular mechanisms responsible for periodontal disease (PD) and its worsening in type 1 Diabetes Mellitus (DM1) remain unknown. Cytokine profile expression levels of collagenases, Mmp14, tissue inhibitors were determined, as the numbers neutrophils macrophages combined streptozotocin-induced DM1 ligature-induced PD models. Increased IL-23 (80-fold) Mmp8 (25-fold) was found DM1. Ligature resulted an IL-1β/IL-6 profile, increased Mmp8, Mmp13, Mmp14 (but not Mmp1), transient Timp1 Reck...
Alveolar bone resorption results from the inflammatory response to periodontal pathogens. Systemic diseases that affect host response, such as type 1 diabetes mellitus (DM1), can potentiate severity of disease (PD) and accelerate resorption. However, biological mechanisms by which DM1 modulates PD are not fully understood. The aim this study was determine influence on alveolar evaluate role receptor activator nuclear factor-kappaB ligand (RANKL)/osteoprotegerin (OPG) in osteoclastogenesis...
Periodontal disease corresponds to a group of lesions that affect the tooth-supporting tissues present in dental follicle. Although bacterial plaque is important, immune response also contributes destruction periodontal tissues. Diabetes mellitus closely associated with development, progression and severity because it not only affects extracellular matrix organization but tissue inflammation. The objective investigation was study influence diabetes on experimental by evaluating degradation...
An alternative label-free electrochemical immunosensor for the rapid detection of Leishmania braziliensis was developed by immobilizing a peptide-based probe promastigote surface antigen (PSA-38S) onto electrospun polyamide-6 (PA6)/chitosan nanofibers. increase in chitosan content spinning solution leads to decrease diameter formed fibers, whereas differential scanning calorimetry (DSC) and X-ray diffraction (XRD) data showed crystallinity upon increasing formulation. In addition,...
Serological testing is a powerful tool in epidemiological studies for understanding viral circulation and assessing the effectiveness of virus control measures, as case SARS-CoV-2, pathogenic agent COVID-19. Immunoassays can quantitatively reveal concentration antiviral antibodies. The assessment antibody titers may provide information on exposure, changes IgG levels are also indicative reduction circulation. In this work, we describe serological study evaluation IgM antibodies their...
Soybean rust is a disease that occurs on soybean leaves and considered very aggressive, reducing product quality. Early identification of fungus in the plants prevents severe farming losses as well spreading to neighboring cultures. In this paper, label-free immunosensor was developed based impedance measurements detect Asian leaf extract at early stages disease. The antibody anti-mycelium Phakopsora pachyrhizi (disease agent) immobilized gold substrate via self-assembled monolayer (SAM)...
This work reports the in vitro expression of Citrus leprosis virus C (CiLV-C) putative coat protein (p29) and production a polyclonal antibody to be used as tool for serological diagnosis citrus leprosis. The ORF2/RNA1, corresponding p29, was cloned pET28a transformed into Escherichia coli cells (BL21). Expression p29 induced purified immunization rabbits produce antibody. anti serum shown highly specific CiLV-C detection by immunological methods (Western blot, PTA-ELISA, tissue blot situ...
Freeze-drying of nanoparticle suspensions in presence pre-formed protein corona is capable generating stable nanoformulations with improved biocompatibility, longer storage times and easier transportation.
Obesity, a burgeoning global health crisis, has tripled in prevalence over the past 45 years, necessitating innovative research methodologies. Adipocytes, which are responsible for energy storage, play central role obesity. However, most studies this field rely on animal models or adipocyte monolayer cell cultures, limited their ability to fully mimic complex physiology of living organism, pose challenges terms cost, time consumption, and ethical considerations. These limitations prompt...
Abstract A precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients’ prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% specificity 100% the most recommended test detect anti-aquaporin-4 antibodies (AQP4-Ab). Here, we tested four AQP4 external loop peptides (AQP4 61–70 , 131–140 141–150 201–210 ) an atomic force microscopy nanoimmunosensor develop diagnostic assay. We obtained highest...
Plants not only evolve but also reduce oxygen in photosynthesis. An inevitable consequence of this normal process is the production reactive species (ROS). are adequately protected by presence multiple antioxidative enzymes cytosol and different cell organelles such as chloroplasts, mitochondria, peroxisomes. Traditionally, ROS were considered to be a toxic byproduct aerobic metabolism. However, recently it has become apparent that plants actively produce these molecules which may control...
Abstract Background: Mineral trioxide aggregate (MTA) has been used in a variety of surgical and non‐surgical endodontic applications. The aim this study was to evaluate the gene expression protein production TNF‐α, IL‐1β IL‐6, as well RANKL OPG using both commercial experimental MTA macrophage cell cultures. Methods: Peritoneal culture performed. Viability, cytokines, OPG, levels experimental‐ commercial‐grey co‐cultured with peritoneal macrophages determined by tryptan blue, real time PCR...
In this work, we considered the autoantibodies proposed as putative biomarkers of demyelination taking into account their reactivity towards myelin oligodendrocyte glycoprotein (MOG) and basic protein (MBP). These proteins are among most commonly researched targets in immunopathology demyelinating diseases. context, development assays for autoantibody detection can contribute a predictive value early diagnosis Hence, aimed to address application silver nanoparticles (AgNPs) sensing device...
Citrus tristeza virus (CTV) is one of the most important citrus pathogen, and among Brazilian CTV strains, genotype Capão Bonito (CB) harmful. Therefore, coat protein (CP) gene were cloned expressed as recombinant used to develop four specific monoclonal antibodies (MAbs). Our previously data had showed these MAbs could recognize different strains present goal identify epitopes CP by ELISA screening overlapping peptides determine binding specificity isolates in light their antigenic domains...
Abstract Antigen-antibody interaction is crucial in autoimmune disease pathogenesis, as multiple sclerosis and neuromyelitis optica. Given that, autoantibodies are essential biomolecules, of which the myelin oligodendrocyte glycoprotein (MOG) can figure a target. Here we combined Molecular Dynamics (MD), Steered (SMD), Atomic Force Microscope (AFM) to detail MOG recognition by its specific antibody. The complex model consisted external domain interacting with an experimental anti-MOG...
A NAM, comprising a microphysiological system of three organ equivalents (skin, liver and intestine), combined with toxicological gene signature analysis, is promising tool to assess chemical safety.