A5032693800- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Venomous Animal Envenomation and Studies
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Rabies epidemiology and control
- Ion channel regulation and function
- Advanced Chemical Sensor Technologies
- Antifungal resistance and susceptibility
- Diphtheria, Corynebacterium, and Tetanus
- Healthcare and Venom Research
- Protease and Inhibitor Mechanisms
- Enzyme Production and Characterization
- Analytical Chemistry and Chromatography
- Celiac Disease Research and Management
- Animal Genetics and Reproduction
- Muscle metabolism and nutrition
- Urologic and reproductive health conditions
- Meat and Animal Product Quality
- Microbial Metabolism and Applications
- Protein Hydrolysis and Bioactive Peptides
- Transgenic Plants and Applications
- Genetically Modified Organisms Research
- Biochemical effects in animals
Centro Universitário Una
2024
Waters (United States)
2011-2023
Universidade Estadual de Campinas (UNICAMP)
2006-2019
Applications Research (United States)
2010-2018
Waters (United Kingdom)
2011
Universidade Federal de São Paulo
2010
Universidade Federal de Alfenas
2010
Instituto Sapientia
2010
Fertility Medical Group
2010
We have studied the behavior of isomers and analogues by traveling wave ion mobility mass spectrometry (TWIM-MS) using drift-gases with varying masses polarizabilities. Despite reduced length cell (18 cm), a pair constitutional isomers, N-butylaniline para-butylaniline, theoretical collision cross-section values in helium (ΩHe ) differing as little 1.2 Å(2) (1.5%) but possessing contrasting charge distribution, showed baseline peak-to-peak resolution (Rp-p for their protonated molecules,...
The use of mass spectrometry to identify recombinant proteins that are expressed in total soluble (TSPs) from plant extracts is necessary accelerate further processing steps. For example, the method consists TSP sample preparation and trypsin digestion prior preliminary characterization using nanoUPLC-MS(E) analysis samples transgenic soybean seeds. A as small 50 μg can be effectively analyzed. In this study, seeds cancer testis antigen (CTAG) were used. procedure covered 30% protein...
The development of somatic cells in to embryogenic occurs several stages and ends embryo formation, though most these biochemical molecular changes have yet be elucidated. Somatic embryogenesis coupled with genetic transformation could a biotechnological tool improve potential crop yields sugarcane cultivars. objective this study was observe identify differentially expressed proteins (E) non-embryogenic (NE) callus during maturation treatment. E NE were cultured on culture medium...
Breast cancer is one of the most commonly diagnosed types among women. mortality rates remain high probably because its diagnosis hampered by inaccurate detection methods. Since changes in protein expression as well modifications glycosylation have been frequently reported development, aim this work was to study differential proteins from plasma women with breast at different stages disease (n = 30) compared healthy 10). A proteomics approach used that depleted albumin and IgG followed...
Chronic myeloid leukemia (CML) is a pluripotent hematopoietic disorder that currently considered incurable. The tyrosine kinase product of the Philadelphia chromosome, P210 BCR-ABL, provided pathogenetic explanation for initiation CML chronic phase and molecular therapeutic target disease. Imatinib mesylate, an orally available BCR-ABL inhibitor, can induce haematologic cytogenetic remission CML. However, imatinib resistance occurs frequently, resulting in relapse. New treatment strategies...
Tyrosine metabolism has an intense role in the synthesis of neurotransmitters. Our study used untargeted, sportomics-based analysis urine samples to investigate changes during a soccer match 30 male junior professional players. Samples were collected before and after analyzed using liquid chromatography mass spectrometry. Results showed significant tyrosine metabolism. Exercise caused downregulation homogentisate metabolites 4-maleylacetoacetate succinylacetone 20% (p = 4.69E-5) 16%...
In this work, sunflowers (Helianthus annuus L.) were cultivated using soil and vermicompost as substrate, plant irrigation was carried out either a Zn solution or mixed ions (Cd, Cu, Pb Zn). After harvesting, the effects of metal-ion contamination on proteins expression (either up- down-regulation) in sunflower leaves evaluated two-dimensional electrophoresis (2-DE), gel images mass spectrometry (MALDI-QTOF MS). When added to nine showed different expressions. Another twenty-three protein...
Corynebacterium pseudotuberculosis is a pathogen classified into two biovars: C. biovar ovis, the etiologic agent of caseous lymphadenitis and equi, which causes ulcerative lymphangitis. The available whole genome sequences different strains have enabled identify difference genes related both virulence physiology each biovar. To evaluate be this could reflect at proteomic level to better understand shared factors exclusive ones ovis equi strains, we applied label-free quantitative...
Corynebacterium pseudotuberculosis biovar ovis is a facultative intracellular pathogen, and the etiological agent of caseous lymphadenitis in small ruminants. During infection process, bacterium subjected to several stress conditions, including nitrosative stress, which caused by nitric oxide (NO). In silico analysis genome C. 1002 predicted genes that could influence resistance this pathogen stress. Here, we applied high-throughput proteomics using high definition mass spectrometry...
Corynebacterium pseudotuberculosis biovar ovis, a facultative intracellular pathogen, is the etiologic agent of caseous lymphadenitis in small ruminants. During infection process, C. changes its gene expression to resist different types stresses and evade immune system host. However, factors contributing infectious process this pathogen are still poorly documented. To better understand identify potential which could be involved virulence, experimental was carried out murine model using...