A5034263524- Venomous Animal Envenomation and Studies
- Rabies epidemiology and control
- Sugarcane Cultivation and Processing
- Biofuel production and bioconversion
- Antimicrobial Peptides and Activities
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Biochemical and Structural Characterization
- Plant nutrient uptake and metabolism
- Vector-Borne Animal Diseases
- Parasitic Infections and Diagnostics
- Marine Invertebrate Physiology and Ecology
- Amphibian and Reptile Biology
- Ion channel regulation and function
- Genomics and Phylogenetic Studies
- Healthcare and Venom Research
- Congenital heart defects research
- Trypanosoma species research and implications
- Tissue Engineering and Regenerative Medicine
- Bacteriophages and microbial interactions
- Entomological Studies and Ecology
- Escherichia coli research studies
- Epigenetics and DNA Methylation
- Bioinformatics and Genomic Networks
Instituto Butantan
2016-2025
Instituto Vital Brazil (Brazil)
2019-2020
Instituto de Biologia Molecular do Paraná
2020
Universidade Federal de São Paulo
2020
Universidade Federal do Paraná
2020
Universidade Federal do Rio de Janeiro
2020
University of California, San Diego
2020
Ezequiel Dias Foundation
2020
Universidade de São Paulo
2006-2019
University of California, Davis
2018
Abstract Transcription factors (TFs) are major players in gene regulatory networks and interactions between TFs their target genes furnish spatiotemporal patterns of expression. Establishing the architecture requires gathering information on TFs, targets genome, corresponding binding sites. We have developed GRASSIUS (Grass Regulatory Information Services) as a knowledge-based Web resource that integrates promoters across grasses. In its initial implementation, consists two separate, yet...
Because of the economical relevance sugarcane and its high potential as a source biofuel, it is important to understand how this crop will respond foreseen increase in atmospheric [CO(2)]. The effects increased [CO(2)] on photosynthesis, development carbohydrate metabolism were studied (Saccharum ssp.). Plants grown at ambient (approximately 370 ppm) elevated 720 during 50 weeks open-top chambers. plants under CO(2) showed, end such period, an about 30% photosynthesis 17% height, accumulated...
Sucrose content is a highly desirable trait in sugarcane as the worldwide demand for cost-effective biofuels surges. Sugarcane cultivars differ their capacity to accumulate sucrose and breeding programs routinely perform crosses identify genotypes able produce more sucrose. mature internodes reach around 20% of culms dry weight. Genotypes populations reflect genetic program may display contrasting growth, development, physiology, all which affect carbohydrate metabolism. Few studies have...
Abstract Background Sugarcane is an increasingly economically and environmentally important C4 grass, used for the production of sugar bioethanol, a low-carbon emission fuel. originated from crosses Saccharum species noted its unique capacity to accumulate high amounts sucrose in stems. Environmental stresses limit enormously sugarcane productivity worldwide. To investigate transcriptome changes response environmental inputs that alter yield we cDNA microarrays profile expression 1,545 genes...
ABSTRACT Background Sugarcane cultivars are polyploid interspecific hybrids of giant genomes, typically with 10–13 sets chromosomes from 2 Saccharum species. The ploidy, hybridity, and size the genome, estimated to have >10 Gb, pose a challenge for sequencing. Results Here we present gene space assembly SP80-3280, including 373,869 putative genes their potential regulatory regions. alignment single-copy in diploid grasses indicates that could resolve 2–6 (up 15) homo(eo)logs 99.1%...
Sugarcane is the source of sugar in all tropical and subtropical countries becoming increasingly important for bio-based fuels. However, its large (10 Gb), polyploid, complex genome has hindered based breeding efforts. Here we release largest most diverse set sugarcane sequences to date, as part an on-going initiative provide a genomic information resource, with ultimate goal producing gold standard genome. Three hundred seventeen chiefly euchromatic BACs were sequenced. A reference one...
Abstract Efforts to describe toxins from the two major families of venomous snakes (Viperidae and Elapidae) usually reveal proteins belonging few structural types, particular each family. Here we carried on an effort determine uncommon cDNAs that represent possible new Lachesis muta (Viperidae). In addition nine classes typical toxins, atypical molecules never observed in hundreds Viperidae studied so far are highly expressed: a diverging C-type lectin is related but appears be independently...
Abstract Background Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. The have different phenotypes affect species. causative agent for type A Xanthomonas citri subsp. , whose genome sequence was made available in 2002. fuscans aurantifolii strain B causes C Results We sequenced genomes strains to draft status. compared their genomic content X. other genomes, with special emphasis III secreted...
Comparisons between venoms from snakes kept under captivity or collected at the natural environment are of fundamental importance in order to obtain effective antivenoms treat human victims snakebites. In this study, we compared composition and biological activities Bothrops atrox venom Tapajós National Forest (Pará State, Brazil) maintained for more than 10 years Instituto Butantan herpetarium after have been mostly Maranhão Brazil. Venoms captive wild were similar except small quantitative...
Background Except for the northern region, where Amazonian black scorpion, T. obscurus, represents predominant and most medically relevant scorpion species, Tityus serrulatus, Brazilian yellow is widely distributed throughout Brazil, causing envenoming fatalities due to sting. In order evaluate compare diversity of venom components obscurus we performed a transcriptomic investigation telsons (venom glands) corroborated by shotgun proteomic analysis from two species. Results The putative...
Phoneutria nigriventer is one of the largest existing true spiders and few considered medically relevant. Its venom contains several neurotoxic peptides that act on different ion channels chemical receptors vertebrates invertebrates. Some these toxins have been shown as promising models for pharmaceutical or biotechnological use. However, large diversity predominance low molecular weight in this hampered identification deep investigation less abundant proteins with high weight. Here, we...
Venom is a key adaptive innovation in snakes, and how nonvenom genes were co-opted to become part of the toxin arsenal significant evolutionary question. While this process has been investigated through phylogenetic reconstruction sequences, evidence provided by genomic context remains less explored. To investigate recruitment, we sequenced genome
Sugarcane is an important sugar and energy crop that can be used efficiently for biofuels production. The development of sugarcane cultivars tolerant to drought could allow the expansion plantations sub-prime regions. Knowledge on mechanisms underlying responses its relationship with carbon partition would greatly help define routes increase yield. In this work we studied using a custom designed oligonucleotide array 21,901 different probes. oligoarrays were contain probes detect...
Multiomics analysis reveals the changes that occur in extracellular matrix brains of Zika virus–infected newborns.
Toxoplasmosis is an alarming public health problem that affects more than one-third of the world's population. In our work, we investigated antiparasitic effects catalytically active [BpMP-I and Jararhagin (Jar)] inactive [Jararhagin-C (Jar-C)] snake venom metalloproteinases (SVMPs) in human HeLa cells. These toxins impaired parasite invasion intracellular growth, modulated IL-6, IL-8, MIF cytokines control cell susceptibility response against T. gondii. Furthermore, verified antiprotozoal...
Dermonecrosis resulting from Loxosceles spider envenomation, primarily driven by the enzyme sphingomyelinase D (SMase D), is characterized severe inflammation and nonhealing wounds. SMases can be classified as Class I or II based on their structural characteristics. exhibits greater dermonecrotic activity than II; however, intracellular mechanisms responsible for this difference remain poorly understood. The differential transcriptomics analysis of human keratinocytes treated with each toxin...
Viral metagenomics has expanded significantly in recent years due to advancements next-generation sequencing, establishing it as the leading method for identifying emerging viruses. A crucial step is taxonomic classification, where sequence data assigned specific taxa, thereby enabling characterization of species composition within a sample. Various classifiers have been developed years, each employing distinct classification approaches that produce varying results and abundance profiles,...
The largest ever recorded epidemic of the Chikungunya virus (CHIKV) broke out in 2004 and affected four continents. Acute symptomatic infections are typically associated with onset fever often debilitating polyarthralgia/polyarthritis. In this study, a systems biology approach was adopted to analyze blood transcriptomes adults acutely infected CHIKV. Gene signatures that were viral RNA levels symptoms identified. Among these genes, putative role Eukaryotic Initiation Factor (eIF) family...
Commercial sugarcane (Saccharum hybrid) is a highly polyploid and aneuploid grass that stores large amounts of sucrose in its stem. We have measured circadian rhythms sense antisense transcription commercial cultivar (RB855453) using custom oligoarray with 14,521 probes hybridize to transcripts (SS) 7,380 (AS).We estimated 32% SS 22% AS were rhythmic. This higher proportion rhythmic than the usually found similar experiments other plant species. Orthologs inparalogs Arabidopsis thaliana,...