A5081336394- Advanced Proteomics Techniques and Applications
- Bioinformatics and Genomic Networks
- Alzheimer's disease research and treatments
- Melanoma and MAPK Pathways
- Mosquito-borne diseases and control
- Health, Environment, Cognitive Aging
- Viral Infections and Vectors
- Neuroinflammation and Neurodegeneration Mechanisms
- Virology and Viral Diseases
- Salivary Gland Disorders and Functions
- Oral microbiology and periodontitis research
- vaccines and immunoinformatics approaches
- Tryptophan and brain disorders
- Amyotrophic Lateral Sclerosis Research
- Immunotherapy and Immune Responses
- Machine Learning in Bioinformatics
- S100 Proteins and Annexins
- Cutaneous Melanoma Detection and Management
- Nuclear Structure and Function
- Dental Health and Care Utilization
- Cellular transport and secretion
- Neurogenetic and Muscular Disorders Research
- Periodontal Regeneration and Treatments
- Antioxidant Activity and Oxidative Stress
- Fibroblast Growth Factor Research
Lund University
2021-2024
Universidade Federal do Rio de Janeiro
2015-2021
Skåne University Hospital
2021
This paper summarizes the recent activities of Chromosome-Centric Human Proteome Project (C-HPP) consortium, which develops new technologies to identify yet-to-be annotated proteins (termed "missing proteins") in biological samples that lack sufficient experimental evidence at protein level for confident identification. The C-HPP also aims forms may be caused by genetic variability, post-translational modifications, and alternative splicing. Proteogenomic data integration basis C-HPP's...
Multiomics analysis reveals the changes that occur in extracellular matrix brains of Zika virus–infected newborns.
Abstract The MM500 meta‐study aims to establish a knowledge basis of the tumor proteome serve as complement genome and transcriptome studies. Somatic mutations their effect on have been extensively characterized in melanoma. However, effects these genetic changes proteomic landscape impact cellular processes melanoma remain poorly understood. In this study, quantitative mass‐spectrometry‐based analysis is interfaced with pathological characterization, associated clinical data. landscape,...
Schizophrenia is a chronic and incurable neuropsychiatric disorder that affects about one percent of the world population. The proteomic characterization synaptosome fraction orbitofrontal cortex useful for providing valuable information molecular mechanisms synaptic functions in these patients. Quantitative analyses proteins were made with eight paranoid schizophrenia patients pool healthy controls free mental diseases. Label-free iTRAQ labeling identified total 2018 protein groups....
Schizophrenia is a chronic disease characterized by the impairment of mental functions with marked social dysfunction. A quantitative proteomic approach using iTRAQ labeling and SRM, applied to characterization mitochondria (MIT), crude nuclear fraction (NUC), cytoplasm (CYT), can allow observation dynamic changes in cell compartments providing valuable insights concerning schizophrenia physiopathology. Mass spectrometry analyses orbitofrontal cortex from 12 patients 8 healthy controls...
Abstract The MM500 study is an initiative to map the protein levels in malignant melanoma tumor samples, focused on in‐depth histopathology coupled proteome characterization. and localization were determined for a broad spectrum of diverse, surgically isolated tumors originating from multiple body locations. More than 15,500 proteoforms identified by mass spectrometry, which chromosomal subcellular was annotated within both primary metastatic melanoma. data generated global proteomic...
Abstract Group A streptococcus (GAS) is a major bacterial pathogen responsible for both local and systemic infections in humans. The molecular mechanisms that contribute to disease heterogeneity remain poorly understood. Here we show the transition from GAS infection paralleled by pathogen-driven alterations IgG homeostasis. Using animal models combination of sensitive proteomics glycoproteomics readouts, documented progressive accumulation cleavage products plasma, due extensive enzymatic...
One Sentence Summary Proteomics analysis lead us to suspect the presence of a Bovine-like viral diarrhea virus (BVDV-like) in brain tissue fetuses bearing microcephaly during outbreak Paraiba State, Brazil, 2015. Abstract No direct experimental causal evidence confirms that Zika is sole etiological agent responsible for development malformations human pregnancy. We used discovery-driven approach analyze protein extracts three positive brains. Shotgun mass spectrometry (MS) proteomics did not...
The discovery of novel protein biomarkers in melanoma is crucial. Our introduction formalin-fixed paraffin-embedded (FFPE) tumor protocol provides new opportunities to understand the progression and open possibility screen thousands FFPE samples deposited biobanks available at hospital pathology departments. In our retrospective biobank pilot study, 90 from 77 patients were processed. Protein quantitation was performed by high-resolution mass spectrometry validated histopathologic analysis....
The hippocampus is a primary region affected in Alzheimer's disease (AD). Because AD postmortem brain tissue not available prior to symptomatic stage, we lack understanding of early cellular pathogenic mechanisms. To address this issue, examined the origin and progression pathogenesis by comparing patient-based model systems including iPSC-derived cells transplanted into mouse hippocampus. Proteomic analysis graft enabled identification pathways network dysfunction patient cells, associated...
Quantitative proteome analysis of four developmental stages pericarp tissues the açaí berry (Euterpe oleracea Mart.) was performed by isobaric labeling peptides with iTRAQ 4-plex, hydrophilic interaction liquid chromatography pre-fractionation labeled peptides, and high-performance mass spectrometry analysis. This resulted in identification 4286 proteins, which 476 presented differential abundance between stages. The these proteins seen to be coordinated metabolic demands during cell...
Abstract Purpose Zika virus (ZIKV) transmission to the fetus during pregnancy could enable a collection of severe fetal malformations like microcephaly (MC), termed Congenital Syndrome (CZS). The mechanisms involved in ZIKV transplacental are not fully understood. Experimental Design Here we aim identify placental tissues deregulated proteins associated with ZIKV‐induced MC using label‐free proteomics. Results We found DNA damage and gene expression inhibition up‐regulated infected placentas...
Alzheimer's disease (AD) is a neurodegenerative disorder and the most common cause of dementia worldwide. In AD, neurodegeneration spreads throughout different areas central nervous system (CNS) in gradual predictable pattern, causing progressive memory decline cognitive impairment. Deposition neurofibrillary tangles (NFTs) specific CNS regions correlates with severity AD constitutes basis for classification into Braak stages (I-VI). Early clinical symptoms are typically associated III-IV...
Background: Hutchinson-Gilford Progeria Syndrome (HGPS) is an extremely rare genetic disorder characterized by accelerated aging and high incidence of cardiovascular complications metabolic alterations. However, the underlying mechanisms this syndrome are not fully understood. Methods Results: This study modelled HGPS using cardiomyocytes generated from induced pluripotent stem cells (iPSc) derived a patient affected syndrome, characterizing molecular biophysical alterations found in...
Abstract The hippocampus is a primary region affected in Alzheimer’s disease (AD). Because AD postmortem brain tissue not available prior to symptomatic stage, we lack understanding of early cellular pathogenic mechanisms. To address this issue, examined the origin and progression pathogenesis patient-based model systems including iPSC-derived cells transplanted into mouse hippocampus. Notably, proteomic analysis graft enabled identification alterations patient cells, associated with...
Abstract Astrocytes play an important role in the onset and progression of amyotrophic lateral sclerosis (ALS), a fatal disorder characterized by relentless degeneration motor neurons (MNs) central nervous system. Despite evidence showing that ALS astrocytes are toxic to MNs, little is understood about earliest pathological changes lead their neurotoxic phenotype. In this study, we generated human from induced pluripotent stem cells (iPSCs) harboring ALS-associated A4V mutation superoxide...
Abstract Zika virus (ZIKV) infection during pregnancy could cause a set of severe abnormalities in the fetus known as congenital syndrome (CZS). Experiments using animal models and vitro systems significantly contributed to our understanding physiopathology ZIKV infection. However, molecular basis CZS is not yet studied humans. Here, we used biology approach integrate transcriptomic, proteomic genomic data from post-mortem brains neonates with CZS. We observed that collagen genes were...