A5088372878- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Lung Cancer Research Studies
- Melanoma and MAPK Pathways
- Peptidase Inhibition and Analysis
- Pancreatic and Hepatic Oncology Research
- vaccines and immunoinformatics approaches
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Bioinformatics and Genomic Networks
- Alzheimer's disease research and treatments
- Cutaneous Melanoma Detection and Management
- Histone Deacetylase Inhibitors Research
- Molecular Biology Techniques and Applications
- Computational Drug Discovery Methods
- Lung Cancer Treatments and Mutations
- Microfluidic and Capillary Electrophoresis Applications
- GDF15 and Related Biomarkers
- Immunotherapy and Immune Responses
- Mitochondrial Function and Pathology
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- Analytical Chemistry and Chromatography
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
Lund University
2016-2025
Korea Research Institute of Chemical Technology
2023
Institute for Biomedicine
2014
University of Pecs
2002-2011
Uppsala University
2008
Abstract The tissue distribution and prognostic relevance of subtype‐specific proteins (ASCL1, NEUROD1, POU2F3, YAP1) present an evolving area research in small‐cell lung cancer (SCLC). expression transcription factors P53 RB1 were measured by immunohistochemistry (IHC) 386 surgically resected SCLC samples. Correlations between vitro efficacy various therapeutic agents investigated proteomics cell viability assays 26 human lines. Besides SCLC‐A (ASCL1‐dominant), SCLC‐AN (combined...
Resistance mechanisms against antiangiogenic drugs are unclear. Here, we correlated the antitumor and antivascular properties of five different receptor tyrosine kinase inhibitors (RTKIs) (motesanib, pazopanib, sorafenib, sunitinib, vatalanib) with their intratumoral distribution data obtained by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). In first mouse model, only sunitinib exhibited broad-spectrum activities simultaneously suppressing vascular...
The hippocampus is important for memory formation and severely affected in the brain with Alzheimer disease (AD). Our understanding of early pathogenic processes occurring hippocampi AD limited due to tissue unavailability. Here, we report a chemical approach rapidly generate free-floating hippocampal spheroids (HSs), from human induced pluripotent stem cells. When used model AD, both APP atypical PS1 variant HSs displayed increased Aβ42/Aβ40 peptide ratios decreased synaptic protein levels,...
Extracellular vesicles (EVs) are submicron, membrane-enclosed particles that released from cells in various pathophysiological states. The molecular cargo of these is considered to reflect the composition cell origin, and EV proteome therefore a potential source biomarkers for diseases. Our aim was determine whether EVs isolated plasma provide additional diagnostic value or improved understanding compared alone context myocardial infarction (MI). A panel proximity extension assays (n = 92)...
Drug therapy is often directed to specific organ and tissue compartments where the mode of action compound affects specifically targeted biological processes. However, direct measurement drug uptake in terms a time kinetic concentrations attained at local sites has not been readily available as clinical index for most drugs. A proof-of-principle study was conducted test utility applying matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) demonstrate qualitative...
This paper summarizes the recent activities of Chromosome-Centric Human Proteome Project (C-HPP) consortium, which develops new technologies to identify yet-to-be annotated proteins (termed "missing proteins") in biological samples that lack sufficient experimental evidence at protein level for confident identification. The C-HPP also aims forms may be caused by genetic variability, post-translational modifications, and alternative splicing. Proteogenomic data integration basis C-HPP's...
// Dingyuan Hu 1, 2 , Daniel Ansari 3 Krzysztof Pawłowski 4, 5 Qimin Zhou 1 Agata Sasor 6 Charlotte Welinder 7 Theresa Kristl Monika Bauden Melinda Rezeli 8 Yi Jiang György Marko-Varga and Roland Andersson Lund University, Faculty of Medicine, Department Clinical Sciences (Surgery), Lund, Sweden Gastroenterology, The Second Affiliated Hospital Wenzhou Medical Wenzhou, China Skane University Hospital, 4 Experimental Design Bioinformatics, Warsaw Life Sciences, Warsaw, Poland Translational...
Melanoma is a highly heterogeneous disease, and deeper molecular classification essential for improving patient stratification treatment approaches. Here, we describe the histopathology-driven proteogenomic landscape of 142 treatment-naïve metastatic melanoma samples to uncover subtypes clinically relevant biomarkers. We performed an integrative analysis identify proteomic subtypes, assess impact BRAF V600 mutations, study profiles cellular composition tumor microenvironment. Clinical...
Extracellular vesicles (ECVs), including microparticles and exosomes, are submicrometer membrane released by diverse cell types upon activation or stress. Circulating ECVs potential reservoirs of disease biomarkers, the complexity these is significantly lower compared to their source, blood plasma, which makes ECV-based biomarker studies more promising. Proteomic profiling important not only discover new diagnostic prognostic markers but also understand roles in biological function. In...
A targeted mass spectrometric assay was developed for identification and quantification of apoE isoforms (apoE2, E3 E4), it utilized screening samples from AD patients (n = 39) with other neurodegenerative disorders 38). The showed good linearity LOQ corresponds to total concentration 0.8 40 ng/mL in CSF plasma/serum, respectively. We identified phenotypes 100% accuracy clinical samples. found strong association between genotypes the individuals their levels blood; ϵ4 allele carriers had...
Melanoma of the skin is sixth most common type cancer in Europe and accounts for 3.4% all diagnosed cancers. More alarming degree recurrence that occurs with approximately 20% patients lethally relapsing following treatment. Malignant melanoma a highly aggressive metastases rapidly extend to regional lymph nodes (stage 3) distal organs 4). Targeted oncotherapy one standard treatment progressive stage 4 melanoma, BRAF inhibitors (e.g. vemurafenib, dabrafenib) combined MEK inhibitor...
Abstract Huntington's disease is a neurodegenerative disorder caused by CAG expansions in the huntingtin (HTT) gene. Modelling challenging, as rodent and cellular models poorly recapitulate seen ageing humans. To address this, we generated induced neurons through direct reprogramming of human skin fibroblasts, which retain age-dependent epigenetic characteristics. (HD-iNs) displayed profound deficits autophagy, characterized reduced transport late autophagic structures from neurites to soma....
Abstract Background Early metastasis is a hallmark of small cell lung cancer (SCLC). However, the mechanisms and resulting patterns SCLC dissemination are unclear. Our aim was thus to investigate organ specificity timing blood‐borne metastases in comprehensive large cohort patients. Methods In this retrospective non‐interventional cross‐sectional study 1009 Caucasian patients, we investigated correlation between distinct locations primary tumor metastatic sites. Results The onset bone ( p...
Abstract The MM500 meta‐study aims to establish a knowledge basis of the tumor proteome serve as complement genome and transcriptome studies. Somatic mutations their effect on have been extensively characterized in melanoma. However, effects these genetic changes proteomic landscape impact cellular processes melanoma remain poorly understood. In this study, quantitative mass‐spectrometry‐based analysis is interfaced with pathological characterization, associated clinical data. landscape,...
Abstract Purpose: Acquired chemoresistance is a frequent event in small cell lung cancer (SCLC), one of the deadliest human malignancies. Histone deacetylase inhibitors (HDACi) have been shown to synergize with different chemotherapeutic agents including cisplatin. Accordingly, we aimed investigate dual targeting HDAC inhibition and chemotherapy SCLC. Experimental Design: The efficacy HDACi SCLC was investigated both vitro vivo. Synergistic drug interactions were calculated based on HSA...
Biobanks are a major resource to access and measure biological constituents that can be used monitor the status of health disease, both in unique individual samples within populations. Most "omic" activities rely on these collections stored provide basis for establishing ranges frequencies expression. Furthermore, information about relative abundance form protein found provides an important historical index comparative studies inherited, epidemic, developing disease. Standardizations sample...
Prostate specific antigen (PSA) is a well-established tumor marker that frequently employed as model biomarker in the development and evaluation of emerging quantitative proteomics techniques, partially result wide access to commercialized immunoassays serving "gold standards." We designed multiple reaction monitoring (MRM) assay detect PSA proteoforms clinical samples (<i>n</i> = 72), utilizing specificity sensitivity method. report, for first time, proteoform coded by SNP-L132I (rs2003783)...