A5033393526- Lung Cancer Treatments and Mutations
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Cancer Mechanisms and Therapy
- Peptidase Inhibition and Analysis
- Advanced Chemical Physics Studies
- Lung Cancer Research Studies
- Gastric Cancer Management and Outcomes
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Neuropeptides and Animal Physiology
- Immunotherapy and Immune Responses
- Protein Hydrolysis and Bioactive Peptides
- Analytical Chemistry and Chromatography
- Ferroptosis and cancer prognosis
- Glycosylation and Glycoproteins Research
- Bioinformatics and Genomic Networks
- Atomic and Molecular Physics
- Molecular Biology Techniques and Applications
- Ion-surface interactions and analysis
- Advanced Glycation End Products research
- Melanoma and MAPK Pathways
- Neuroendocrine Tumor Research Advances
- Computational Drug Discovery Methods
- Atmospheric chemistry and aerosols
St. Marianna University School of Medicine
2016-2024
Nishijin Hospital
2024
Tokyo Medical University
2007-2022
Daiichi University of Pharmacy
2022
University School
2022
National Hospital Organization
2022
Jichi Medical University
2022
Jichi Medical University Hospital
2022
Tohoku University
2022
National Institute for Materials Science
2022
Human umbilical cord blood (CB) is a potential source for mesenchymal stem cells (MSC) capable of forming specific tissues, example, bone, cartilage, or muscle. However, difficulty isolating MSC from CB (CB-MSC) has impeded their clinical application. Using more than 450 units donated to two public banks, we found that successful cell recovery fits hyper-exponential function time since birth with very high fidelity. Additionally, significant improvement in the isolation CB-MSC was achieved...
Protein ubiquitylation contributes to the regulation of many cellular processes including protein degradation, receptor internalization, and repair DNA damage. We now present a comprehensive characterization ubiquitin-conjugated ubiquitin-associated proteins in human cells. The were purified by immunoaffinity chromatography under denaturing or native conditions. They then digested with trypsin, resulting peptides analyzed 2-D LC MS/MS. A total 670 distinct identified; 345 (51%) classified as...
The objective of the international Chromosome-Centric Human Proteome Project (C-HPP) is to map and annotate all proteins encoded by genes on each human chromosome. C-HPP consortium was established organize a collaborative network among research teams responsible for protein mapping individual chromosomes identify compelling biological genetic mechanisms influencing colocated their products. aims foster development proteome analysis integration findings from related molecular -omics...
To investigate aberrant plasma proteins in lung cancer, we compared the proteomic profiles of serum from five cancer patients and four healthy volunteers. Immuno-affinity chromatography was used to deplete highly abundant proteins, resulting samples were separated into eight fractions by anion-exchange chromatography. Quantitative protein fractionated generated two-dimensional difference gel electrophoresis, which experimental internal control labeled with different dyes co-separated...
CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily. Studies with human B cells show that binding CD154 (gp39, CD40L) to recruits TNF receptor– associated 2 (TRAF2) and TRAF3 complex, induces downregulation nonreceptor-associated TRAFs in cell an increased expression Fas on surface. Combined signaling through interluekin 4 commensurate increase level TRAF2, but not TRAF3, recruited complex. In contrast, engagement membrane immunoglobulin limits upregulation reduces...
Abstract Pancreatic cancer is among the most lethal malignancies worldwide. We aimed to identify novel prognostic markers by applying mass spectrometry (MS)‐based proteomic analysis formalin‐fixed paraffin‐embedded (FFPE) tissues. Resectable, node positive pancreatic ductal adenocarcinoma (PDAC) with poor ( n = 4) and better outcomes, based on survival duration, essentially same clinicopathological backgrounds, noncancerous ducts 5) were analyzed. Cancerous cells collected from FFPE tissue...
Abstract aly is a unique spontaneous autosomal recessive mutation in mice that causes deficiency the systemic lymph nodes (LN) and Peyer's patches (PP). also induces abnormal histological findings spleen humoral cell‐mediated immune functions, although lymphocytes of aly/aly mouse show virtually normal responses vitro . We studied structure detail by immunohistochemistry electron microscopy. found was deficient expression specific antigens for marginal metallophils (MM), zone macrophages...
Thymocytes from DO10 T-cell-receptor transgenic mice undergo apoptosis, or programmed cell death, when chicken ovalbumin-(323-339) peptide is administered in vivo. Using thymocytes, we have now developed a simple vitro model system that recapitulates the vivo clonal-deletion process. When thymocytes were cocultured with fibroblasts, B cells, thymic nurse lines (all bearing I-Ad) presence of ovalbumin-(323-339), deletion TCR+CD4+CD8+ was seen within 8-20 hr. designed to bear I-Ad on their...
In clinical and diagnostic proteomics, it is essential to develop a comprehensive robust system for proteome analysis. Although multidimensional liquid chromatography/tandem mass spectrometry (LC/MS/MS) systems have been recently developed as powerful tools especially identification of protein complexes, these still some drawbacks in their application research that requires an analysis large number human samples. Therefore, this study, we constructed technically simple high throughput...
We performed a global protein expression study on soft-tissue sarcoma in order to develop novel diagnostic biomarkers and allow molecular classification. 2-D difference gel electrophoresis was used generate the profiles of 80 samples with seven different histological backgrounds. found that 67 spots distinguished subtypes sarcoma. Hierarchical clustering these resulted grouping all corresponding pattern tropomyosin isoforms conventional pleomorphic leiomyosarcomas. also identified five...
BACKGROUND: Annexin II (ANX2) is a multi-functional protein involved in cell proliferation and membrane physiology related to cancer progression. The purpose of this study was assess ANX2 expression clear-cell (cc) renal carcinoma (RCC). METHODS: 18 primary ccRCCs examined by real-time reverse transcriptase (RT)–PCR western blot analyses. Furthermore, immunohistochemical performed using paraffin section 154 24 metastases. association between the clinicopathological factors prognosis...
Melanoma of the skin is sixth most common type cancer in Europe and accounts for 3.4% all diagnosed cancers. More alarming degree recurrence that occurs with approximately 20% patients lethally relapsing following treatment. Malignant melanoma a highly aggressive metastases rapidly extend to regional lymph nodes (stage 3) distal organs 4). Targeted oncotherapy one standard treatment progressive stage 4 melanoma, BRAF inhibitors (e.g. vemurafenib, dabrafenib) combined MEK inhibitor...
Abstract The MM500 meta‐study aims to establish a knowledge basis of the tumor proteome serve as complement genome and transcriptome studies. Somatic mutations their effect on have been extensively characterized in melanoma. However, effects these genetic changes proteomic landscape impact cellular processes melanoma remain poorly understood. In this study, quantitative mass‐spectrometry‐based analysis is interfaced with pathological characterization, associated clinical data. landscape,...
Personalized medicine allows the selection of treatments best suited to an individual patient and disease phenotype. To implement personalized medicine, effective tests predictive response treatment or susceptibility adverse events are needed, develop a test, both high quality samples reliable data required. We review key features state-of-the-art proteomic profiling introduce further analytic developments build toolkit for use in approaches. The combination novel analytical approaches...
Abstract Purpose: We aimed to identify candidate proteins for tumor markers predict the response gefitinib treatment. Experimental Design: did two-dimensional difference gel electrophoresis create protein expression profile of lung adenocarcinoma tissues from patients who showed a different used support vector machine algorithm select that best distinguished 31 responders 16 nonresponders. The prediction performance selected spots was validated by an external sample set, including six and...
Purpose The molecular underpinnings that may prognosticate survival and increase our understanding of tumor development progression are still poorly understood. This study aimed to define the signatures for malignancy in small cell lung carcinoma (SCLC), which is known its highly aggressive clinical features poor prognosis. Experimental design Using specimens, authors perform a comparative proteomic analysis high‐grade SCLCs low‐grade pulmonary carcinoid tumors (PCTs), both types...
Abstract Biallelic variants in the EYS gene are a major cause of autosomal recessive inherited retinal disease (IRD), with high prevalence Asian population. The purpose this study was to identify pathogenic variants, determine clinical/genetic spectrum -associated ( -RD), and discover disease-associated relatively allele frequency (1%-10%) nationwide Japanese cohort. Sixty-six affected subjects from 61 families biallelic or multiple pathogenic/disease-associated were ascertained by...