A5031004030- Plant tissue culture and regeneration
- Wheat and Barley Genetics and Pathology
- Transgenic Plants and Applications
- Plant Genetic and Mutation Studies
- Food composition and properties
- Lung Cancer Treatments and Mutations
- Advanced Proteomics Techniques and Applications
- Rice Cultivation and Yield Improvement
- Plant Reproductive Biology
- Fermentation and Sensory Analysis
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Spaceflight effects on biology
- Agriculture, Soil, Plant Science
- Horticultural and Viticultural Research
- Neuroendocrine Tumor Research Advances
- Adenosine and Purinergic Signaling
- DNA Repair Mechanisms
- Food Quality and Safety Studies
- Erythrocyte Function and Pathophysiology
- Lung Cancer Research Studies
- Molecular Biology Techniques and Applications
- Pancreatic function and diabetes
- Peptidase Inhibition and Analysis
- Enzyme Production and Characterization
Asahi Breweries (Japan)
2007-2024
Machida Endoscope (Japan)
2011-2015
Osaka University
1997-2000
Cornell University
1997
The initiation of DNA synthesis is an important cell cycle event that defines the beginning S phase. This critical involves participation proteins whose functions are regulated by cyclin dependent protein kinases (Cdks). Mcm2–7 a family six conserved essential for in all eukaryotes. In Saccharomyces cerevisiae, members undergo cycle-specific phosphorylation. Phosphorylation Mcm at phase coincides with removal these from chromatin and onset synthesis. this study, we identified DBF4, which...
Abstract Pancreatic cancer is among the most lethal malignancies worldwide. We aimed to identify novel prognostic markers by applying mass spectrometry (MS)‐based proteomic analysis formalin‐fixed paraffin‐embedded (FFPE) tissues. Resectable, node positive pancreatic ductal adenocarcinoma (PDAC) with poor ( n = 4) and better outcomes, based on survival duration, essentially same clinicopathological backgrounds, noncancerous ducts 5) were analyzed. Cancerous cells collected from FFPE tissue...
The yeast <i>Saccharomyces cerevisiae</i>Cdc7p/Dbf4p protein kinase complex was purified to near homogeneity from insect cells. efficiently phosphorylated Mcm2p and less the remaining Mcm proteins or other replication proteins. Significantly, when pretreated with alkaline phosphatase, became completely inactive as a substrate, suggesting that it must be by kinase(s) substrate for Cdc7p/Dbf4p complex. Mutant complexes containing either Cdc7-1p Dbf4-1∼5p were also partially cells characterized...
Barley intake reportedly reduces the risk of cardiovascular disease, but effects on systemic phenotypes during healthy aging have not yet been examined. Therefore, we examined barley lifespan; behavioral phenotypes, such as locomotor activity, and cognitive functions, intestinal microbiome in senescence-accelerated mouse-prone 8 (SAMP8) mouse. We prepared two mild high-fat diets by adding lard, which starch components AIN-93G were replaced rice or "Motchiriboshi." SAMP8 (four weeks old,...
Background There are no reports of proteomic analyses inflamed islets in type 1 diabetes. Procedures Proteins expressed the enterovirus-associated fulminant diabetes (FT1DM) with extensive insulitis were identified by laser-capture microdissection mass spectrometry using formalin-fixed paraffin-embedded pancreatic tissues. Results Thirty-eight proteins solely FT1DM islets, most which have not been previously linked to Five protein-protein interacting clusters identified, and cellular...
Large cell neuroendocrine carcinoma (LCNEC) of the lung, a subtype large (LCC), is characterized by differentiation that small lung (SCLC) shares. Pre-therapeutic histological distinction between LCNEC and SCLC has so far been problematic, leading to adverse clinical outcome. We started project establishing protein targets characteristic with proteomic method using formalin fixed paraffin-embedded (FFPE) tissues, which will help make diagnosis convincing.Cancer cells were collected laser...
Interstitial lung disease (ILD) events have been reported in Japanese non-small-cell cancer (NSCLC) patients receiving EGFR tyrosine kinase inhibitors. We investigated proteomic biomarkers for mechanistic insights and improved prediction of ILD. Blood plasma was collected from 43 gefitinib-treated NSCLC developing acute ILD (confirmed by blinded diagnostic review) 123 randomly selected controls a nested case-control study within pharmacoepidemiological cohort Japan. generated ∼7 million...
Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic inflammation of the synovial joints. Early intervention followed early diagnosis can result in remission; however, both stage and provision effective treatment have been impeded heterogeneity RA, which details pathological mechanism are unclear. Regardless numerous investigations RA means genomic proteomic approaches, proteins interplaying tissues that contain various types synoviocytes, not yet sufficiently...
Pancreatic cancer is among the most lethal malignancies worldwide. This study aimed to identify a novel prognostic biomarker, facilitating treatment selection, using mass spectrometry (MS)-based proteomic analysis with formalin-fixed paraffin-embedded (FFPE) tissue.The two groups poor prognosis (n = 4) and better had been carefully chosen 96 resected cases of pancreatic during 1998 2007 in Tohoku University Hospital. Although those 2 adjusted background (UICC-Stage IIB, Grade2, R0,...
β-Amylase (α-1,4-glucan maltohydrolase; EC 3.2.1.2) is known to be an important enzyme in beer brewing. To identify the contribution of β-amylase more detail, two β-amylase-deficient barley, 'Backcrossed line (BCL)-Haruna' and 'Nittakei 81', were used for investigation malt quality brewing characteristics. 'BCL-Haruna' was bred by backcross method introduction trait from landrace barley ('Tibet Violet 1') Japanese traditional malting 'Haruna Nijo'. 81' crossing between 'Sukai Golden' 'Tibet...
Putative nudix hydrolase (NUDX) genes, which encode amino acid sequences showing homology with those of Arabidopsis NUDXs and conserve motif, were identified from barley. The 14 deduced barley (HvNUDX1-14) classified into established subfamilies, except for 8-oxo-deoxyguanosine 5'-triphosphate (8-oxo-dGTP) pyrophosphohydrolase mRNA decapping enzyme three substrate-unknown subfamilies. Drought UV-C stresses, respectively, up-regulated 7 4 HvNUDX but some homologs showed different responses to...
In Japan, rising costs have impacted the framework of maintaining an efficient and effective healthcare system. Today, urgent implementation programs to address this need led a rebuilding entire approach medical evaluation clinical care. Recent developments in proteomics based on mass spectrometry (MS) for identifying, sequencing, quantifying disease-relevant protein biomarkers is promising means optimal drug prescription using biomarker diagnosis. We illustrate report our experience with...
Changes in saccharide, amino acid and S-methylmethionine (SMM) concentrations enzyme activities during the malting of barley grown with different nitrogen (N) sulfur (S) supplementation were investigated order to clarify their relationship N S fertiliser levels.Concentrations grain significantly increased by addition culture soil. Application decreased starch concentration grain. On other hand, higher fertilisation β-glucan malt, thus decreasing accessibility β-glucanase its substrates....
The gene expression and enzyme activity of superoxide dismutase, catalase, ascorbate peroxidase in the space-grown barley were not significantly different from those ground-grown barley. Cu2+ reducing radical scavenging activities an extract lower than by 0.7 fold, suggesting that space environment does induce oxidative stress, reduces antioxidant capacity plants.
Epoetin beta pegol (continuous erythropoiesis receptor activator; C.E.R.A.), or methoxy-polyethylene glycol-modified epoetin beta, is a long-acting stimulating agent (ESA) that effectively maintains hemoglobin levels. It promotes proliferation of erythroid progenitor cells in hematopoietic organs and leads to increased reticulocyte However, the detailed erythropoietic effects various ESAs on their target have yet be clarified, new approaches are needed analyze tissue iron localization with...
Arabinoxylan is a major polysaccharide found in barley and wheat. As this has been reported to be involved wort viscosity filterability, as well β-glucan, it become increasingly important beer brewing recent years, despite the limited availability of measurement methodology for arabinoxylan. In study, we developed simple method determining arabinoxylan content products using commercially available xylose determination kit. The results show relationship between products. These were consistent...
An overview on targeted personalized medicine is given describing the developments in Japan of lung cancer patients. These new therapies with novel drugs require implementations, order to follow and monitor drug efficacy outcome. Examples from IRESSA (Gefitinib) TARCEVA (Erlotinib) treatments used medication patients are presented. Lung one most common causes mortality world. The importance both quantification disease progression, where diagnostic-related biomarkers being implemented,...