Effects of transforming growth factor beta (TGF-beta) on IgA production by LPS-stimulated B cells have been studied. TGF-beta itself could augment polyclonal in concomitant inhibition IgM and IgG1 production. Furthermore, IL-5 additively augmented exerted its activity early the culture (by 2 d a 5-d culture) was required late culture. Surface IgA- (sIgA-) responded to for development IgA-secreting cells. By contrast, sIgA+ cells, but not sIgA- These results suggest that has differential role...

Genes for the human alpha 5(IV) and 6(IV) collagen chains have a unique arrangement in that they are colocalized on chromosome Xq22 head-to-head fashion appear to share common bidirectional promoter. In addition we reported novel observation COL4A6 gene is transcribed from two alternative promoters tissue-specific manner (Sugimoto, M., T. Oohashi, Y. Ninomiya. 1994. Proc. Natl. Acad. Sci. USA. 91:11679-11683). To know whether translation products of both genes various tissues, raised...

Spaceflight environment have been shown to generate reactive oxygen species (ROS) and induce oxidative stress in plants, but little is known about the gene expression of ROS network plants grown long-term spaceflight. The molecular response adaptation spaceflight Mizuna harvested after 27 days cultivation onboard International Space Station (ISS) were measured using genome-wide mRNA analysis (mRNA-Seq). Total reads transcripts from ISS as well on ground by mRNA-Seq showed 8,258 14,170...

An Arabidopsis cDNA encoding putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The comprised 803 bp included an open reading frame which encodes a polypeptide of 169 amino acid residues. deduced sequence showed about 80 50% homology with plant PHGPXs mammalian PHGPXs, respectively. Southern blot analysis suggested that the PHGPX gene single-copy gene. expression profile in under NaCl Al/Fe treatments, generate oxidative stress, analyzed. Northern...

To date, five distinct a chains have been identified in basement membrane collagen IV.W e cloned gene encoding new chain belonging to IV by cDNA isolation under low stringency conditions.Isolation of overlapping clones and the technique 6' rapid amplification ends enabled us derive primary structure entire polypeptide.The deduced polypeptide contained 1678 amino acid residues, including 21-residue signal peptide, 24-residue amino-terminal NC domain, central 1406-residue collagenous (COL1)...

The genes for the alpha 5(IV) and 6(IV) chains of human basement membrane collagen type IV have been found together on chromosome X at segment q22 reported to be arranged in a head-to-head fashion. Here we report 5' flanking sequences COL4A5 COL4A6 that is transcribed from two alternative promoters tissue-specific Analysis sequence immediately upstream transcription start sites revealed some features housekeeping genes--i.e., lack TATA motif presence CCAAT CTC boxes. Further analysis...

Chédiak-Higashi syndrome (CHS) in children can be a fatal disease. We describe the oldest known CHS patient first seen with neurologic disorder early adult life. From age of 22, this 39-year-old woman developed mental deterioration, parkinsonism including resting tremor at tongue, mandible, and hands, oculogyric crisis, muscular atrophy limbs, loss tendon reflexes. MRI showed marked temporal dominant brain diffuse spinal cord atrophy. Partial albinism, panleukocytic giant granules,...

The gene encoding L-methionine gamma-lyase from Pseudomonas putida was cloned and the primary structure of enzyme deduced its nucleotide sequence. expressed in Escherichia coli. amino acid sequences BrCN-digested peptides agreed with corresponding parts sequence determined structure. polypeptide is composed 398 residues a calculated molecular weight 42,626, to subunit homotetrameric enzyme. only showed extensive homology other well known alpha,gamma-elimination and/or gamma-replacement...

Acid- and aluminum (Al)-tolerant microorganisms were isolated from tea fields, which six strains selected identified as Cryptococcus humicola, Rhodotorula glutinis, Aspergillus flavus Link, Penicillium sp., janthinellum Biourge Trichoderma asperellum. They tolerant to Al up 100–200 mM could grow at low pH, 2.5–2.2. In a glucose medium (pH 3.5) the pH of spent decreased below 3.0. The toxic inorganic monomeric in with three (A. F-6b, sp. F-8b P. F-13), but total remained constant for all...

The ocular manifestation of human T-cell lymphotropic virus type I (HTLV-I) infection has been recognized as a new clinical entity termed HTLV-I uveitis. In order to determine whether uveitis is associated with lymphocyte alveolitis, bronchoalveolar lavage (BAL) was carried out in 11 patients uveitis, five asymptomatic carriers, HTLV-I-negative sarcoidosis, and nine normal control subjects seronegative for HTLV-I. Six the showed increased total cell counts, T-lymphocytosis BAL fluid....

We have used a series of Escherichia coli-expressed deletion mutants the glycoprotein (GP) Ib-binding domain von Willebrand factor (vWF) to study structural basis its function. In addition prototypic molecule (rvWF441-733), we constructed 11 mutants; seven had deletions sequence on amino- and/or carboxyl-terminal side Cys509-Cys695 intrachain disulfide loop, and four limited inside loop. Other cysteine residues in 509 695, when present corresponding native sequence, were mutated glycine; all...

Mammalian zona pellucida is an attractive target for developing a contraceptive vaccine.In this study, the amino acid sequence of core protein porcine glycoprotein ZP4 was determined by peptide mapping and cDNA cloning.Two kinds peptides were identified: one consisted 128 residues other 133 with additional five at carboxy-terminal end peptide.Both had two potential N-linked glycosylation sites.The showed 39.1% similarity to amino-terminal region mouse ZP2 polypeptide.The positions cysteine...

Pulmonary alveolar macrophages were obtained by saline lavage from 23 healthy male volunteers--10 non-smokers and 13 cigarette smokers. Lavage produced three times as many in smokers than non-smokers. When incubated vitro, more cells adhered to glass, spread out, showed enhanced nitroblue tetrazolium (NBT) reduction. The surface morphology of with a plate like appearance ridge membrane surface, while the predominantly spherical ruffles. proportions which stained highly for beta galactosidase...

We first isolated and characterized genomic DNA fragments that cover the 5' flanking sequences of COL4A3 COL4A4 encoding human basement membrane alpha3(IV) alpha4(IV) collagen chains, respectively. Nucleotide sequence analysis indicated two genes are arranged head-to-head. To determine transcription start site for gene, we performed RACE RNase protection assays, indicating there alternative transcripts presumably derived from different promoters. Interestingly, one (from exon 1') is only 5...

ABSTRACT Several Sphingomonas spp. utilize polyethylene glycols (PEGs) as a sole carbon and energy source, oxidative PEG degradation being initiated by dye-linked dehydrogenase (PEG-DH) that oxidizes the terminal alcohol groups of polymer chain. Purification characterization PEG-DH from terrae revealed enzyme is membrane bound. The gene encoding this ( pegA ) was cloned, sequenced, expressed in Escherichia coli . purified recombinant vulnerable to aggregation inactivation, but could be...

The strongest fibrinolytic protease (F-III-2) in the six enzyme proteins purified from earthworm, Lumhricus rubellus [N. Nakajima et al., Biosci. Biotech. Biochem., 57, 1726–1730 (1993)] has been modified chemically with fragmented human serum albumin (mol. wt., 10,000–30,000). lost antigenicity of native and reacted antisera against albumin, fragments, conjugate to form precipitation lines, which fused each other. was significantly more resistant inactivation by inhibitors rat plasma. a...

A cDNA encoding spinach putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The included an open reading frame that encoded a polypeptide of 171 amino acid residues. deduced sequence showed about 77 50% similarity to plant PHGPXs mammalian PHGPXs, respectively. PCR product with the same size as PHGPX were obtained from maize, soybeans, Arabidopsis, suggesting expression genes in other plants.