A5054700020- Mosquito-borne diseases and control
- Viral Infections and Vectors
- COVID-19 Impact on Reproduction
- Virology and Viral Diseases
- Pregnancy and preeclampsia studies
- Cervical Cancer and HPV Research
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Endometrial and Cervical Cancer Treatments
- Thyroid and Parathyroid Surgery
- Global Maternal and Child Health
- Intestinal and Peritoneal Adhesions
- Systemic Lupus Erythematosus Research
- Viral Infections and Outbreaks Research
- Maternal and fetal healthcare
- Reproductive System and Pregnancy
- Hernia repair and management
- COVID-19 and healthcare impacts
- Complement system in diseases
- Teratomas and Epidermoid Cysts
- Congenital Heart Disease Studies
- Phytochemistry Medicinal Plant Applications
- Toxoplasma gondii Research Studies
- Nasal Surgery and Airway Studies
- Global Cancer Incidence and Screening
Universidade do Estado do Rio de Janeiro
2005-2024
Fundação Oswaldo Cruz
2014-2024
Hospital Universitário Pedro Ernesto
2024
Faculdades Oswaldo Cruz
2022-2023
Universidade Federal do Rio de Janeiro
2022
Instituto Nacional de Saúde
2019
Pontifical Catholic University of Rio de Janeiro
2016
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2013
Genetic Analysis (Norway)
2013
Universidade Federal do Estado do Rio de Janeiro
2003
Perinatal death is an increasingly important problem as the coronavirus disease 2019 (COVID-19) pandemic continues, but mechanism of has been unclear.To evaluate role placenta in causing stillbirth and neonatal following maternal infection with COVID-19 confirmed placental positivity for severe acute respiratory syndrome 2 (SARS-CoV-2).Case-based retrospective clinicopathologic analysis by a multinational group 44 perinatal specialists from 12 countries autopsy pathology findings 64...
In Brazil, an epidemic of Zika virus (ZIKV) infections was declared in 2015 that coincided with alarming reports microcephaly newborns associated mother infection. Although the has placental tropism, changes tissue morphology and immunity infected patients have not yet been elucidated. Here, we investigated histopathological ultrastructural along immunological profile BDNF expression rare material. Tissues were obtained 2015-2016 Brazilian epidemic, ten ZIKV-infected during pregnancy, five...
Multiomics analysis reveals the changes that occur in extracellular matrix brains of Zika virus–infected newborns.
SARS-CoV-2 infection during pregnancy is not usually associated with significant adverse effects. However, in this study, we report a fetal death mild COVID-19 34-week-pregnant woman. The virus was detected the placenta and an unprecedented way several tissues. Placental abnormalities (MRI anatomopathological study) were consistent intense vascular malperfusion, probably cause of death. Lung histopathology also showed signs inflammation, which could have been contributory factor. Monitoring...
During the Zika epidemic in Brazil, a baby was born at term with microcephaly and arthrogryposis. The mother had symptoms 10 weeks of gestation. At 17 weeks, ultrasound showed cerebral malformation ventriculomegaly. 24 amniotic fluid contained ZIKV RNA birth, placenta maternal blood were also positive using RT-qPCR. birth urine RNA, whereas CSF days did not. Seizures started 6 days. EEG abnormal CT scan atrophy, calcifications, lissencephaly, ventriculomegaly, cerebellar hypoplasia....
High-risk human papillomaviruses are closely associated with cervical cancer and its precursor lesions through interactions between the E6 E7 oncoproteins cell-cycle regulatory proteins, such as p53 pRb, respectively. As other molecules involved in control seem to be important for papillomavirus (HPV)-mediated carcinogenesis, we have analyzed expression of p53, p21, p16, cyclin D1, Ki-67 presence HPV (HPV pool HPV-16) by immunohistochemical studies using tissue microarray low squamous...
Visceral leishmaniasis (VL) is the most severe form of and second major cause death by parasites, after malaria. The arsenal drugs against small, each has a disadvantage in terms toxicity, efficacy, price, or treatment regimen. Our group focused on studying new drug candidates as alternatives to current treatments. pterocarpanquinone LQB-118 was designed synthesized based molecular hybridization, it exhibited antiprotozoal anti-leukemic cell line activities. previous work demonstrated that...
Host factors that influence Congenital Zika Syndrome (CZS) outcome remain elusive. Interferons have been reported as the main antiviral factor in and other flavivirus infections. Here, we accessed samples from 153 pregnant women (77 without 76 with CZS) 143 newborns 66 exposed to ZIKV conducted a case-control study verify whether interferon alfa receptor 1 (IFNAR1) lambda 2 4 (IFNL2/4) single nucleotide polymorphisms (SNPs) contribute CZS outcome, characterized placenta gene expression...
Zika virus (ZIKV) infections during pregnancy can result in Congenital Syndrome (CZS), a range of severe neurological outcomes fetuses that primarily occur early gestational stages possibly due to placental damage. Although some placentas maintain ZIKV persistence for weeks or months after the initial infection and diagnosis, impact this viral is still unknown. Here, we aimed investigate immunological repercussion term placentas. As such, from 64 pregnant women diagnosed with different...
Abstract There are limited data on amniocentesis as a diagnostic tool for congenital Zika syndrome. Here we report prospective cohort of 16 women with suspected virus infection in highly endemic area, and discuss the role prenatal diagnosis fetal infection.
Zika-exposed infants with microcephaly (proportional or disproportional) and those who are small for gestational age without should be closely followed, particularly their growth trajectories. They at high risk of adverse outcomes in the first year life.Antenatal Zika virus (ZIKV) exposure may lead to infant including being (SGA). ZIKV-exposed a diagnosis [PM] disproportional [DM]) SGA birth were evaluated anthropometric measurements health outcomes.
Abstract Previous work showed that the thymus can be infected by RNA viruses as HIV and HTLV-1. We thus hypothesized might also Zika virus (ZIKV). Herein we provide compelling evidence ZIKV targets human thymic epithelial cells (TEC) in vivo vitro . ZIKV-infection enhances keratinization of TEC, with a decrease proliferation increase cell death. Moreover, modulates high amount coding RNAs upregulation genes related to adhesion migration, well non-coding including miRNAs, circRNAs lncRNAs....
The CDKN1A gene product is a p53 downstream effector, which participates in cell differentiation, development process, repair, apoptosis, senescence, migration, and tumorigenesis. objective of our study was investigated the importance two polymorphisms gene, rs1801270 (31C>A) rs1059234 (70C>T), for cervical lesions Southeastern Brazilian population (283 cases, stratified by lesion severity, 189 controls). genotyping performed polymerase chain reaction-restriction fragment length polymorphism...
There are limited data on the natural history of antenatal Zika virus (ZIKV) exposure in twin pregnancies, especially regarding intertwin concordance prenatal, placental, and infant outcomes.
Intracerebral hemorrhage associated with vaccine-induced thrombotic thrombocytopenia
The interplay between cervical cancer (CC) and immune cells, mainly intratumoral lymphocytes, has a pivotal role in carcinogenesis. In this context, we evaluated the distribution of CD45RA+ CD45RO+ cells as well CCR6+ CCL20+ intraepithelial (IE) marginal stroma (MS) areas from neoplasia (CIN) I-III, CC 'immunoscore' for HPV-induced outcome. We observed increased IE MS group compared to CIN groups healthy volunteers. Interestingly, there is remarkable reduction expressing according lesion...
Variants of p16(INK4a) and p14(ARF), encoded by the CDKN2A locus, may respond differently to presence human papillomavirus (HPV). We investigated potential association two polymorphisms, 500C > G (rs11515) 540C T (rs3088440), with cervical neoplasia in patients lesions healthy controls (n = 492). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), single-strand conformation (SSCP) and/or DNA sequencing techniques were employed for genotyping. The 500G allele was...
Congenital Zika Syndrome (CZS) is a critical illness with wide range of severity caused by virus (ZIKV) infection during pregnancy. Life-threatening neurodevelopmental dysfunctions are among the most common phenotypes observed in affected newborns. Risk factors that contribute to susceptibility and response ZIKV may be related itself, environment, maternal genetic background. Nevertheless, newborn's contribution still not elucidated. Here, we aimed identify possible variants as well relevant...
Abstract Background The p53 and p21 proteins are important regulators of cell cycle apoptosis may contribute to autoimmune diseases, such as systemic lupus erythematosus (SLE). As genetic polymorphisms cause changes in protein levels functions, we investigated associations TP53 ( CDKN1A ) 72 G > C—rs1042522; PIN3—rs17878362; 31 C A—rs1801270; 70 T—rs1059234) with the development (SLE) a Southeastern Brazilian population. Methods Genotyping 353 female volunteers (cases, n = 145; controls,...
Abstract Background The impact of COVID-19 pandemic in pregnant patients is an important emerging topic. Evidence transplacental transmission and typical histopathological alterations the placenta are controversial literature. Methods This was a prospective observational cohort multicenter study which we selected 23 placentas 21 that showed detection SARS-CoV-2 RNA by RT-PCR tissue described both morphological clinical characteristics. Immunohistochemistry performed to localize virus...
Mitochondria combine hydrogen and oxygen to produce heat adenosine triphosphate (ATP). As a toxic by-product of oxidative phosphorylation (OXPHOS), mitochondria generate reactive species (ROS). These free radicals may cause damage mitochondrial DNA (mtDNA) other molecules in the cell. Nitric oxide (NO) plays an important role biology human cancers, including breast cancer; however, it is still unclear how NO might affect genome. The aim current study determine mtDNA oncogenic process. Using...