A5045226298- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- Cancer-related Molecular Pathways
- COVID-19 Impact on Reproduction
- Immunotherapy and Immune Responses
- Virology and Viral Diseases
- Viral gastroenteritis research and epidemiology
- Long-Term Effects of COVID-19
- Bacterial Infections and Vaccines
- Monoclonal and Polyclonal Antibodies Research
- Bioinformatics and Genomic Networks
- Pneumocystis jirovecii pneumonia detection and treatment
- vaccines and immunoinformatics approaches
- DNA Repair Mechanisms
- COVID-19 epidemiological studies
- Pneumonia and Respiratory Infections
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- Hepatitis Viruses Studies and Epidemiology
- Research on Leishmaniasis Studies
- Bacillus and Francisella bacterial research
- Influenza Virus Research Studies
- Kawasaki Disease and Coronary Complications
Fundação Oswaldo Cruz
2015-2025
Instituto de Tecnologia e Pesquisa
2020-2024
Universidade Federal do Rio de Janeiro
2002-2023
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2023
Universidade de São Paulo
2023
Universidade Federal do Amazonas
2023
Ministério da Saúde
2021
National Institute of Science and Technology for Structural Biology and Bioimaging
2009-2012
Yellow fever virus (YFV) live attenuated vaccine can, in rare cases, cause life-threatening disease, typically patients with no previous history of severe viral illness. Autosomal recessive (AR) complete IFNAR1 deficiency was reported one 12-yr-old patient. Here, we studied seven other previously healthy aged 13 to 80 yr unexplained YFV vaccine–associated disease. One 13-yr-old patient had AR IFNAR2 deficiency. Three vaccinated at the ages 47, 57, and 64 high titers circulating auto-Abs...
The tumor suppressor protein p53 is a nuclear that serves as an important transcription factor. region responsible for sequence-specific DNA interaction located in its core domain (p53C). Although full-length binds to tetramer, p53C monomer since it lacks the oligomerization domain. It has been previously demonstrated two domains have dimerization interface and undergo conformational change when bound DNA. Here we demonstrate with consensus sequence provides of enhanced stability at...
p53 is a transcription factor that maintains genome integrity, and its function lost in 50% of human cancers. The majority mutations are clustered within the core domain. Here, we investigate effects low pH on structure wild-type (wt) domain (p53C) R248Q mutant. At pH, tryptophan residue partially exposed to solvent, suggesting fluctuating tertiary structure. On other hand, secondary increases, as determined by circular dichroism. Binding probe bis-ANS (bis-8-anilinonaphthalene-1-sulfonate)...
Massive vaccination positively impacted the SARS-CoV-2 pandemic, being a strategy to increase titers of neutralizing antibodies (NAbs) in population. Assessing NAb levels and understanding kinetics responses is critical for evaluating immune protection. In this study, we optimized validated PRNT50 assay assess 50% virus neutralization evaluated its accuracy measure NAbs original strain or variant SARS-CoV-2. The optimal settings were selected, such as cell (2 × 105 cells/well) CMC (1.5%)...
In the last decade, Flaviviruses such as yellow fever (YFV) and Zika (ZIKV) have expanded their transmission areas. These viruses originated in Africa, where they exhibit both sylvatic interhuman cycles. Brazil, risk of YFV urbanization has grown, with approaching most densely populated metropolis, while concern about ZIKV spillback to a cycle risen. To investigate these health threats, we carried out extensive collections arbovirus screening 144 free-living, non-human primates (NHPs) 5219...
Lactoferrin (Lf) is an important immunomodulator in infections caused by different agents. During SARS-CoV-2 infection, Lf can hinder or prevent virus access to the intracellular environment. Severe cases of COVID-19 are related increased production cytokines, accompanied a weak type 1 interferon response.
Abstract Since its emergence in late 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been posing a serious threat to public health worldwide as the causative agent of disease 2019 (COVID-19). Now distributed pandemic pattern, this still lacks an effective drug treatment with low toxicity, leading pharmaceutical companies and research labs work against time find candidate molecule efficiently treat affected patients. Due well-known broad-spectrum antimicrobial activity...
Infections caused by SARS-CoV-2 induce a severe acute respiratory syndrome called COVID-19 and have led to more than six million deaths worldwide. Vaccination is the most effective preventative measure, cellular humoral immunity crucial developing individual protection. Here, we aim investigate hybrid against triggered ChAadOx1 nCoV-19 vaccine in Brazilian cohort. We investigated immune response from vaccination naïve (noCOVID-19) previously infected individuals (COVID-19) analyzing levels...
Successful SARS-CoV-2 inactivation allows its safe use in Biosafety Level 2 facilities, and the of whole viral particle helps development analytical methods a more reliable immune response, contributing to improvement vitro vivo assays. In order obtain functional product, we evaluated several protocols observed that 0.03% beta-propiolactone for 24 h was best condition tested, as it promoted above 99.99% no cytopathic effect visualized after five serial passages. Moreover, RT-qPCR...
Fungal infections cause 1.7 million deaths annually, which can be attributed not only to fungus-specific factors, such as antifungal resistance and biofilm formation, but also drug-related challenges. In this study, the potential of Amphotericin (AmB) loaded polymeric nanoparticles (AmB-NPs) combined with murine monoclonal antibodies (mAbs) (i.e., CC5 DD11) was investigated a strategy overcome these To achieve goal, AmB-NPs were prepared by nanoprecipitation using different polymers...
Enveloped animal viruses must undergo membrane fusion to deliver their genome into the host cell. We demonstrate that high pressure inactivates two membrane-enveloped viruses, influenza and Sindbis, by trapping particles in a fusion-intermediate state. The pressure-induced conformational changes Sindbis were followed using intrinsic extrinsic fluorescence spectroscopy, circular dichroism, fusion, plaque, hemagglutination assays. Influenza virus subjected exposes hydrophobic domains as...
Host factors that influence Congenital Zika Syndrome (CZS) outcome remain elusive. Interferons have been reported as the main antiviral factor in and other flavivirus infections. Here, we accessed samples from 153 pregnant women (77 without 76 with CZS) 143 newborns 66 exposed to ZIKV conducted a case-control study verify whether interferon alfa receptor 1 (IFNAR1) lambda 2 4 (IFNL2/4) single nucleotide polymorphisms (SNPs) contribute CZS outcome, characterized placenta gene expression...
Abstract Human Mpox is a highly contagious viral disease caused by virus, currently causing outbreaks in various regions of the world and considered global public health problem. Understanding how virus adapts or transfers to new host species crucial for effective prevention treatment infections. A comprehensive analysis 1,318 accessible genomes was conducted assess potential phenotypic effects, incorporating examination pseudogenes, gene duplication deletion, as well sequences obtained from...
Abstract The re-emergence of yellow fever (YF) urged new mass vaccination campaigns and, in 2017, the World Health Organization approved use fractional dose (FD) YF vaccine due to stock shortage. In an observational cross-sectional investigation, we have assessed viremia, antibodies, soluble mediators and effector memory T B-cells induced by primary volunteers with FD standard (SD). Similar viremia levels antibodies markers were early after immunization. However, a faster decrease latter was...
Zika virus (ZIKV) infections during pregnancy can result in Congenital Syndrome (CZS), a range of severe neurological outcomes fetuses that primarily occur early gestational stages possibly due to placental damage. Although some placentas maintain ZIKV persistence for weeks or months after the initial infection and diagnosis, impact this viral is still unknown. Here, we aimed investigate immunological repercussion term placentas. As such, from 64 pregnant women diagnosed with different...
One patient presented hyporexia, asthenia, adynamia, and jaundice two months after acute yellow fever (YF) onset; plus laboratory tests indicating hepatic cytolysis a rebound of alanine aspartate transaminases, total direct bilirubin levels. Laboratory discarded autoimmune hepatitis, inflammatory or metabolic liver disease, new infections caused by hepatotropic agents. Anti-YFV IgM, IgG neutralizing antibodies were detected in different times, but no viremia. A biopsy was collected three YF...
The yellow fever vaccine (YF17DD) is highly effective with a single injection conferring protection for at least 10 years. YF17DD induces polyvalent responses, TH1/TH2 CD4+ profile, robust T CD8+ and synthesis of interferon-gamma (IFN-γ), culminating in high titers neutralizing antibodies. Furthermore, C-type lectin domain containing 5A (CLEC5A) has been implicated innate outcomes other flaviviral infections. Here, we conducted follow-up study volunteers immunized YF17DD, investigating the...
The present work describes the isolation and purification of two Leishmania chagasi (= syn. infantum) recombinant proteins, rLci2B rLci1A, their use in development an immunoassay for diagnostic canine leishmaniasis. After protein expression cell disruption, was purified by immobilized metal affinity chromatography followed size exclusion chromatography, whereas expressed as inclusion body, treated with urea anion-exchange chromatography. Homogeneities were ascertained denaturing gel...