A5090673357- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- HIV Research and Treatment
- Vaccine Coverage and Hesitancy
- Virology and Viral Diseases
- Viral Infections and Outbreaks Research
- Vibrio bacteria research studies
- COVID-19 epidemiological studies
- vaccines and immunoinformatics approaches
- Neuroinflammation and Neurodegeneration Mechanisms
- Autoimmune and Inflammatory Disorders Research
- Synthesis and Biological Evaluation
- Adenosine and Purinergic Signaling
- Diverticular Disease and Complications
- Inflammasome and immune disorders
- Invertebrate Immune Response Mechanisms
- Complement system in diseases
- Respiratory viral infections research
- Infectious Encephalopathies and Encephalitis
- Bioactive Compounds and Antitumor Agents
- Microscopic Colitis
- Respiratory Support and Mechanisms
Fundação Oswaldo Cruz
2015-2025
Instituto de Tecnologia e Pesquisa
2020-2025
New York Proton Center
2022
Communities In Schools of Orange County
2022
Ann Arbor Center for Independent Living
2022
University of Richmond
2022
Oregon Medical Research Center
2022
Lindsay Unified School District
2022
Finnish Institute for Health and Welfare
2015
New York University Press
2015
Dengue virus (DENV) is spread through most tropical and subtropical areas of the world represents a serious public health problem. At present, control dengue disease mainly hampered by absence antivirals or vaccine, which results in an estimated half worldwide population at risk infection. The immune response against DENV not yet fully understood better knowledge it now recognized as one main challenge for vaccine development. In previous studies, we reported that DNA containing signal...
Massive vaccination positively impacted the SARS-CoV-2 pandemic, being a strategy to increase titers of neutralizing antibodies (NAbs) in population. Assessing NAb levels and understanding kinetics responses is critical for evaluating immune protection. In this study, we optimized validated PRNT50 assay assess 50% virus neutralization evaluated its accuracy measure NAbs original strain or variant SARS-CoV-2. The optimal settings were selected, such as cell (2 × 105 cells/well) CMC (1.5%)...
Zika virus (ZIKV) infection during pregnancy can lead to congenital syndrome (CZS) and may result in neurodevelopmental alterations exposed children, with without CZS. This study aimed evaluate ZIKV as a risk factor for early long-term adverse outcomes. retrospective-prospective, matched cohort was conducted Mato Grosso do Sul, Brazil. Mother-infant pairs unexposed were enrolled the from 2018 2022. Clinical epidemiological data gestational period neonatal evaluations obtained Brazilian...
In 2013, the World Health Organization (WHO) withdrew recommendation of booster doses yellow fever (YF) vaccine, based on retrospective and cross-sectional studies that showed lifelong protective immunity from a single dose. Currently, transmission in Brazil occurs only through jungle (sylvatic) cycle. However, high vector density Aedes aegypti , which transmits other orthoflaviviruses, is concern for expansion YF regions country. We conducted cohort study to assess duration vaccine-induced...
Two DNA vaccines were constructed encoding the ectodomain (domains I, II and III) of DENV2 envelope protein (pE1D2) or only its domain III (pE2D2), fused to human tissue plasminogen activator signal peptide (t-PA). The expression secretion recombinant proteins was confirmed in vitro BHK cells transfected with two plasmids, detected by immunofluorescence immunoprecipitation metabolically labeled gene products, using polyclonal monoclonal antibodies against DENV2. Besides, results reveal that...
In the last decade, Flaviviruses such as yellow fever (YFV) and Zika (ZIKV) have expanded their transmission areas. These viruses originated in Africa, where they exhibit both sylvatic interhuman cycles. Brazil, risk of YFV urbanization has grown, with approaching most densely populated metropolis, while concern about ZIKV spillback to a cycle risen. To investigate these health threats, we carried out extensive collections arbovirus screening 144 free-living, non-human primates (NHPs) 5219...
Infections caused by SARS-CoV-2 induce a severe acute respiratory syndrome called COVID-19 and have led to more than six million deaths worldwide. Vaccination is the most effective preventative measure, cellular humoral immunity crucial developing individual protection. Here, we aim investigate hybrid against triggered ChAadOx1 nCoV-19 vaccine in Brazilian cohort. We investigated immune response from vaccination naïve (noCOVID-19) previously infected individuals (COVID-19) analyzing levels...
Glioblastoma is the most frequent and aggressive primary brain cancer. In preclinical studies, Zika virus, a flavivirus that triggers death of glioblastoma stem-like cells. However, oncolytic activity has not been demonstrated in human patients. Here we report patient who received standard care therapy, including surgical resection, radiotherapy temozolomide. shortly after tumor mass was clinically diagnosed with typical arbovirus-like infection, during virus outbreak Brazil. Following...
Abstract The re-emergence of yellow fever (YF) urged new mass vaccination campaigns and, in 2017, the World Health Organization approved use fractional dose (FD) YF vaccine due to stock shortage. In an observational cross-sectional investigation, we have assessed viremia, antibodies, soluble mediators and effector memory T B-cells induced by primary volunteers with FD standard (SD). Similar viremia levels antibodies markers were early after immunization. However, a faster decrease latter was...
Abstract The present study aimed at evaluating the YF-specific neutralizing antibody profile besides a multiparametric analysis of phenotypic/functional features cell-mediated response elicited by 1/5 fractional dose 17DD-YF vaccine, administered as single subcutaneous injection. immunological parameters each volunteer was monitored two time points, referred as: before (Day 0) [Non-Vaccinated, NV (D0) ] and after vaccination 30–45) [Primary Vaccinees, PV (D30–45) ]. Data demonstrated high...
The dengue envelope glycoprotein (E) is the major component of virion surface and its ectodomain composed domains I, II III. This protein main target for development a vaccine with induction neutralizing antibodies. In present work, we tested two different vaccination strategies, combined immunizations in prime/booster regimen or simultaneous inoculation DNA (pE1D2) chimeric yellow fever/dengue 2 virus (YF17D-D2). pE1D2 encodes DENV2 fused to t-PA signal peptide, while YF17D-D2 was...
Abstract Dengue is an important arboviral infection, causing a broad range symptom that varies from life-threatening mild illness to severe clinical manifestations. Recent studies reported the impairment of central nervous system (CNS) after dengue characteristic previously considered as atypical and underreported. However, little known about neuropathology associated dengue. Since animal models are tools for helping understand pathogenesis, including neurological damages, aim this work was...
Despite the rapid mass vaccination against COVID-19, emergence of new SARS-CoV-2 variants concern, such as omicron, is still a great distress, and therapeutic options are needed. Bovine lactoferrin (bLf), multifunctional iron-binding glycoprotein available in unsaturated (apo-bLf) saturated (holo-bLf) forms, has been shown to exert broad-spectrum antiviral activity many viruses. In this study, we evaluated efficacy both forms bLf at 1 mg/mL infection Vero cells by SARS-CoV-2. As assessed...
Leishmaniasis remains a serious public health problem in developing countries without effective control, whether by vaccination or chemotherapy. Part of the failure leishmaniasis control is due to lack new less toxic and more drugs able eliminate both lesions parasite. Oxiranes derived from naphthoquinones now being assayed are promising for treatment this group diseases. The predicted pharmacokinetic properties toxicological profiles epoxy-α-lapachone epoxymethoxy-lawsone have been compared...
Dengue is a mild flu-like arboviral illness caused by dengue virus (DENV) that occurs in tropical and subtropical countries. An increasing number of reports have been indicating also associated to neurological manifestations, however, little known regarding the neuropathogenesis disease. Here, using BALB/c mice intravenously infected with DENV-2 strain 66985, we demonstrated capable invading damaging host's central nervous system (CNS). Brain cerebellum animals revealed histological...
The present study aimed at investigating whether the hydroxychloroquine (HCQ) treatment would impact neutralizing antibody production, viremia levels and kinetics of serum soluble mediators upon planned 17DD-Yellow Fever (YF) primovaccination (Bio-Manguinhos-FIOCRUZ) primary Sjögren's syndrome (pSS). A total 34 pSS patients 23 healthy controls (HC) were enrolled. group was further categorized according to use HCQ (HCQ Non-HCQ). YF-plaque reduction neutralization test (PRNT ≥1:50), YF...
Background Brazil faced a yellow fever(YF) outbreak in 2016–2018 and vaccination was considered for autoimmune rheumatic disease patients(ARD) with low immunosuppression due to YF high mortality. Objective This study aimed evaluate, prospectively the first time, short-term immunogenicity of fractional vaccine(YFV) immunization ARD patients immunossupression. Methods Results A total 318 participants(159 159 age- sex-matched healthy controls) were vaccinated fractional-dose(one fifth)...
Fractional dose is an important strategy to increase access vaccines. This study evaluated the effectiveness, safety, and immunogenicity of half ChAdOx1 nCoV-19 vaccine. A non-inferiority non-randomized controlled trial compared a with full dose, interval 8 10 weeks, in individuals aged 18–49 years. The primary endpoints were incidence rate new cases/1,000 person-year at 90 days after 14 second confirmed by RT-PCR cases registered SUS National Health Surveillance Database (e-SUS VS)....
Dengue disease has emerged as a major public health issue across tropical and subtropical countries. Infections caused by dengue virus (DENV) can evolve to life-threatening forms, resulting in about 20,000 deaths every year worldwide. Several animal models have been described concerning pre-clinical stages vaccine development against dengue, each of them presenting limitations advantages. Among these models, traditional approach is the inoculation mouse-brain adapted DENV variant...