A5018968727- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Synthesis and Biological Evaluation
- Insect Pest Control Strategies
- Parasites and Host Interactions
- Bioactive Compounds and Antitumor Agents
- Insect Resistance and Genetics
- Nitric Oxide and Endothelin Effects
- Pineapple and bromelain studies
- Venomous Animal Envenomation and Studies
- Phytochemical compounds biological activities
- Chemical Reactions and Mechanisms
- Synthesis and biological activity
- Helminth infection and control
- Toxin Mechanisms and Immunotoxins
- Biochemical and Molecular Research
- Curcumin's Biomedical Applications
- Organic and Inorganic Chemical Reactions
- vaccines and immunoinformatics approaches
- Phenothiazines and Benzothiazines Synthesis and Activities
- Malaria Research and Control
- Enzyme Production and Characterization
- Quinazolinone synthesis and applications
- Eosinophilic Disorders and Syndromes
- Renin-Angiotensin System Studies
Fundação Oswaldo Cruz
2015-2025
Universidad Nacional de Colombia
2010
Universidade Federal Rural do Rio de Janeiro
2003
Macrophages are host cells for the pathogenic parasite Leishmania major. Neutrophils die and ingested by macrophages in tissues. We investigated role of macrophage interactions with inflammatory neutrophils control L. major infection. Coculture dead exudate exacerbated growth infected from susceptible BALB, but killed intracellular resistant B6 mice. Coinjection amplified replication vivo prevented Neutrophil depletion reduced load infection Exacerbated required PGE(2) TGF-beta production...
Leishmania (Leishmania) amazonensis is a protozoan that causes infections with broad spectrum of clinical manifestations. The currently available chemotherapeutic treatments present many problems, such as several adverse side effects and the development resistant strains. Natural compounds have been investigated potential antileishmanial agents, epoxy-α-lapachone on L. (L.) were analyzed in study. This compound was able to cause measurable promastigote amastigote forms parasite, affecting...
ABSTRACT The golden hamster ( Mesocricetus auratus ) is a susceptible model to Leishmania Viannia spp.; however, available studies employ different infection protocols, which account for clinical and pathological presentation differences. Herein, L . V .) braziliensis preparations were standardized contain 10 4 , 5 or 6 parasites determine an optimal inoculum that ensured cutaneous lesions without causing disseminated in hamsters. Lesion development was followed 105 days by size...
Current treatment of cutaneous leishmaniasis includes pentavalent antimonials as first-line drugs, but this therapy has shown severe adverse effects. An alternative to minimize issue is based on combination scheme with other drugs. In study we analyzed the potential association meglumine antimoniate (MA) oxiranes epoxy-α-lapachone (LAP) or epoxymethyl-lawsone (LAW). Results demonstrated that between these drugs enhanced leishmanicidal activity Leishmania (Leishmania) amazonensis infection....
Leishmaniasis is a widespread disease caused by Leishmania spp., affecting millions of people around the world. For decades, its treatment has been based on pentavalent antimonials, which notoriously cause toxic side effects in patients. In this study, epoxy-α-lapachone incorporated into an oil-in-water-type microemulsion (ELAP-ME) and meglumine antimoniate (MA) were assayed monotherapy combination (ELAP-ME/MA) BALB/c mice infected with (Leishmania) amazonensis. general, there was reduction...
Leishmania (Viannia) braziliensis Thor strain exhibits a heterogeneous composition comprised of subpopulations with varying levels infectivity. Clonal were previously obtained from the by sorting single-parasites and proceeding cultivation. The used in this study are named Thor03, 10 Thor22.
Kinetoplastid membrane protein-11 (KMP-11), a protein present in all kinetoplastid protozoa, is considered potential candidate for leishmaniasis vaccine. A suitable vaccine molecule must be expressed amastigotes, the infective stage mammals. However, expression of KMP-11 Leishmania amastigotes has been subject controversy. We evaluated this logarithmic and stationary growth phase promastigotes, as well amazonensis by immunoblotting, flow cytometry immunocytochemistry, using monoclonal...
Epoxymethoxylawsone is a naphthoquinone derivative promising as drug candidate for the treatment of leishmaniases. In present work effectiveness epoxymethoxylawsone, and meglumine antimoniate on Leishmania (Leishmania) amazonensis parasites mice paw lesions infected BALB/c was assessed. an intracellular amastigotes assay, half-maximal inhibitory concentration (IC50) value epoxymethoxylawsone slightly higher (1.7-fold) than that found antimoniate. The efficacy both drugs became more evident...
In a previous works searching for new drugs with high efficiency, we reported the in vitro and vivo antileishmanial activity of series diarylheptanoid structurally related to curcumin against L. amazonensis. This work describes diarylheptanoids diarylpentanoids derivatives. These were assayed Leishmania amazonensis, braziliensis chagasi promastigotes containing percentage metacyclic forms axenic amastigote form amazonensis using Pentamidine Isethionate as reference drug. Parasites log late...
In Leishmania amazonensis, kinetoplastid membrane protein-11 (KMP-11) expression increases during metacyclogenesis and is higher in amastigotes than promastigotes, suggesting a role for this protein the infection of mammalian host. We show that addition KMP-11 exacerbates L. amazonensis peritoneal macrophages from BALB/c mice by increasing interleukin (IL)-10 secretion arginase activity while reducing nitric oxide (NO) production. The doses KMP-11, IL-10 levels intracellular amastigote loads...
Leishmaniasis remains a serious public health problem in developing countries without effective control, whether by vaccination or chemotherapy. Part of the failure leishmaniasis control is due to lack new less toxic and more drugs able eliminate both lesions parasite. Oxiranes derived from naphthoquinones now being assayed are promising for treatment this group diseases. The predicted pharmacokinetic properties toxicological profiles epoxy-α-lapachone epoxymethoxy-lawsone have been compared...
Peptides from the COOH‐terminal extension of cysteine proteinase B Leishmania (Leishmania) amazonensis (cyspep) can modulate immune responses in vertebrate hosts. With this hypothesis as base, we used online analysis tool SYFPEITHI to predict seven epitopes region with potential bind H2 proteins. We performed proliferation tests and quantified reactive T lymphocytes applying a cytometry analysis, using samples draining lymph node lesions L. (L.) ‐infected mice. To define reactivity cells,...
Because of the importance cell signalling processes in proliferation and differentiation, adenylate cyclase pathway was studied, specifically protein kinase A (PKA) Leishmania amazonensis. The PKAs soluble (SF) enriched membrane fractions (MF) from infective/non-infective promastigotes axenic amastigotes were assayed. In order to purify PKA molecule, chromatographed on DEAE-cellulose columns phosphorylative activity evaluated using [gamma(32)P]-ATP as phosphate source. These experiments...
Abstract Background Leishmania (Vianna) braziliensis , (Leishmania) amazonensis and chagasi are important parasites in the scenario of leishmaniasis Brazil. During life cycle these parasites, promastigote forms adhere to midgut epithelial microvillii phlebotomine insects avoid being secreted along with digestive products. Lulo cells a potential model that will help understand features this adhesion phenomenon. Here, we analyze interaction between spp. promastigotes vitro specifically...