A5075163331- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Insect Pest Control Strategies
- Insect Resistance and Genetics
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Reproductive tract infections research
- Synthesis and Biological Evaluation
- Bioactive Compounds and Antitumor Agents
- Malaria Research and Control
- Cervical Cancer and HPV Research
- Insect and Pesticide Research
- Plant Parasitism and Resistance
- Electrocatalysts for Energy Conversion
- Neurobiology and Insect Physiology Research
- Vector-borne infectious diseases
- Psychodrama and Leishmaniasis Studies
- Synthesis and biological activity
- Amoebic Infections and Treatments
- Chemical synthesis and pharmacological studies
- Parasitic Diseases Research and Treatment
- Legume Nitrogen Fixing Symbiosis
- Parasitic Infections and Diagnostics
Fundação Oswaldo Cruz
2015-2024
Universidade do Estado do Rio de Janeiro
2024
Trichomonas vaginalis is the aetiological agent of human trichomoniasis, which one most prevalent sexually transmitted diseases in humans. Iron an important element for survival this parasite and colonisation host urogenital tract.In study, we investigated effects iron on proliferation dynamics pseudocyst formation morphologically characterised depletion-induced pseudocysts.We performed structural ultrastructural analyses using light microscopy, scanning electron microscopy transmission...
Leishmaniasis is a widespread disease caused by Leishmania spp., affecting millions of people around the world. For decades, its treatment has been based on pentavalent antimonials, which notoriously cause toxic side effects in patients. In this study, epoxy-α-lapachone incorporated into an oil-in-water-type microemulsion (ELAP-ME) and meglumine antimoniate (MA) were assayed monotherapy combination (ELAP-ME/MA) BALB/c mice infected with (Leishmania) amazonensis. general, there was reduction...
Leishmania (Viannia) braziliensis Thor strain exhibits a heterogeneous composition comprised of subpopulations with varying levels infectivity. Clonal were previously obtained from the by sorting single-parasites and proceeding cultivation. The used in this study are named Thor03, 10 Thor22.
In American Tegumentary Leishmaniasis production of cytokines, reactive oxygen species and nitric oxide (NO) by host macrophages normally lead to parasite death. However, some Leishmania braziliensis strains exhibit natural NO resistance. NO-resistant cause more lesions are frequently resistant antimonial treatment than NO-susceptible ones, suggesting that parasites endowed with specific mechanisms survival persistence. To tests this, we analyzed the effect pro- antioxidant molecules on...
Abstract Background Aedes albopictus , a ubiquitous mosquito, is one of the main vectors dengue and yellow fever, representing an important threat to public health worldwide. Peptidases play key roles in processes such as digestion, oogenesis, metamorphosis insects. However, most information on proteolytic enzymes mosquitoes derived from insects adult stages often directed towards understanding blood digestion. The aim this study was investigate expression active peptidases preimaginal Ae....
Trichomonas vaginalis is a sexually transmitted anaerobic parasite that infects humans causing trichomoniasis, common and ubiquitous disease. The life cycle of this possesses trophozoite form without cystic stage. However, the presence nonproliferative nonmotile, yet viable reversible spherical forms with internalized flagella, denominated pseudocysts, has been commonly observed for parasite. To understand mechanisms involved in formation we performed mass spectrometry-based high-throughput...
Anopheles aquasalis is a dipteran of the family Culicidae that widely distributed in coastal regions South and Central America. This species acts as vector Plasmodium vivax, an important etiological agent malaria, which represents serious public health problem. In mosquitoes, trypsin-like serine proteases are blood meal digestion, immune responses reproductive functions. The study peptidases expressed mosquito midgut essential to understanding mechanisms parasite-host interaction...
Culex quinquefasciatus is a hematophagous insect from the Culicidae family that feeds on blood of humans, dogs, birds and livestock. This species transmits wide variety pathogens between humans animals. The midgut environment first location pathogen-vector interactions for blood-feeding mosquitoes expression specific peptidases in early stages feeding could influence outcome infection. Trypsin-like serine belong to multi-gene can be expressed different isoforms under distinct physiological...
Leishmania species are responsible for a broad spectrum of diseases, denominated Leishmaniasis, affecting over 12 million people worldwide. During the last decade, there have been impressive efforts sequencing genome most pathogenic spp. as well hundreds strains, but large-scale proteomics analyses did not follow these achievements and proteome remained mostly uncharacterized. Here, we report comprehensive comparative study proteomes strains representing L. braziliensis, panamensis...
Abstract Background Aedes albopictus is widely distributed across tropical and sub-tropical regions associated with the transmission of several arboviruses. Although this species increasingly relevant to public health due its ability successfully colonize both urban rural habitats, favoring dispersion viral infections, little known about biochemical traits, all assumptions made based on studies A. aegypti . In previous we characterized peptidase profile pre-imaginal stages reported first...
Leishmaniasis is a complex group of infectious and parasitic diseases that afflict many thousands individuals across five continents. treatment remains challenge because it relies on drugsknown for their high toxicity limited efficacy, making itimperative to identify new molecules offer greater effectiveness safety. This study sought explore the impact seven synthetic guanidine derivatives (LQOF-G1, LQOF-G2, LQOF-G6, LQOF-G7, LQOF-G32, LQOF-G35 LQOF-G36) onthe parasite Leishmania (Viannia)...
This perspective presents and supports arguments for a new formulation of epoxy-α-lapachone loaded microemulsion (ELAP-ME), nanosystem, as prototype drug the treatment leishmaniasis. The benefits ELAP multitarget compound, with properties that affect key physiological pathways Leishmania spp. are discussed. ELAP-ME demonstrated efficacy in murine infection models, particularly binomial BALB/c-Leishmania (Leishmania) amazonensis. Furthermore, it is proposed technological maturity be...
Abstract Glucantime (Sb V ) is the first-line treatment against American Tegumentary Leishmaniasis. Resistance cases to this drug have been reported and related host characteristics parasite phenotypes. In study, 12 Leishmania ( Viannia braziliensis isolates from patients that presented clinical cure (Responders—R) relapse or therapeutic failure (Non-responders—NR) after with antimony, were analyzed. These parasites assessed by in vitro susceptibility Sb III , serine proteases activity...
Leishmania parasites carry a double-stranded RNA virus (Leishmania - LRV) that has been divided in LRV1 and LRV2.Leishmania (Viannia) braziliensis clinical isolates were assessed order to determine LRV presence.Two-round polymerase chain reaction (PCR nested PCR) was performed detect or LRV2 L. (V.) (n = 12).LRV1 detected three which phylogenetically related other sequences reported from American tegumentary leishmaniasis (ATL) endemic areas of Brazil. Patients infected with LRV-negative...
Lutzomyia longipalpis-derived cell line (Lulo) has been suggested as a model for studies of interaction between sandflies and Leishmania.Here, we present data proteomic gene expression analyses Lulo related to interactions with Leishmania (Viannia) braziliensis.Lulo protein extracts were analysed through combination two-dimensional gel electrophoresis mass spectrometry resulting spots further investigated in silico identify proteins using Mascot search and, afterwards, sequences applied...
Proteases are virulence factors with a recognized impact on the Leishmania spp. life cycle. This study considers set of analyses measuring phenotypic L. (V.) braziliensis clinical isolates as promastigotes growth curves, murine peritoneal macrophages infection, inflammatory mediators production, and serine proteases gene expression (subtilisin 13: S13, subtilisin 28: S28, oligopeptidase B: OPB) assessing these isolates’ fitness in vitro conditions. Parasites had different behavior during...
SUMMARY Iron is an essential element for the survival of trichomonads during host–parasite interaction. The availability this metal modulates several metabolic pathways parasites and regulates expression virulence factors such as adhesins proteolytic enzymes. In study, we investigated effect iron depletion on morphology life cycle Tritrichomonas foetus . Scanning transmission electron microscopy analyses revealed that from culture medium (named TYM-DIP inducer medium) induces morphological...
Visceral leishmaniasis (VL) is a neglected disease caused by Leishmania parasites. Although significant morbidity and mortality in tropical subtropical regions of the world are associated with VL, low investment for developing new treatment measures chronic. Moreover, resistance failure increasing main medications, but emergence phenotypes poorly understood at protein level. Here, we analyzed development to miltefosine upon experimental selection L. infantum strain. Time was ~six months...
During their life cycle, trypanosomatids are exposed to stress conditions and adapt energy antioxidant metabolism colonize hosts. Strigomonas culicis is a monoxenous protist found in invertebrates with an endosymbiotic bacterium that completes essential biosynthetic pathways for the trypanosomatid. Our research group previously generated wild-type H2O2-resistant (WTR) strain showed improved mitochondrial defenses, which led higher rates of Aedes aegypti infection. Here, we assess biological...