In Brazil, an epidemic of Zika virus (ZIKV) infections was declared in 2015 that coincided with alarming reports microcephaly newborns associated mother infection. Although the has placental tropism, changes tissue morphology and immunity infected patients have not yet been elucidated. Here, we investigated histopathological ultrastructural along immunological profile BDNF expression rare material. Tissues were obtained 2015-2016 Brazilian epidemic, ten ZIKV-infected during pregnancy, five...

Alphavirus chikungunya virus (CHIKV) is an arbovirus, belonging to the Togaviridae family. The disease caused by CHIKV generally evolves with spontaneous resolution in a few weeks; however, progression chronic may occur. most common symptoms are fever, myalgia, and arthralgia; skin manifestations occur 40 80% of infected individuals. Morbilliform maculopapular erythematous eruptions, vesiculobullous lesions, generalized erythema, eruption peeling, hypermelanosis, painful oral urticarial...

Introduction The dengue virus (DENV) is the etiological agent that causes fever illness, an arbovirus with a major endemic potential has become increasingly prevalent in Brazil and already been associated fatal cases children. DENV tropism for several organs, including lungs causing pulmonary complications. aim of this article was to evaluate inflammatory histopathological profile lung tissue three children infected DENV, which represents group more susceptible fatality due its incomplete...

Dengue is an important mosquito-borne disease and a global public health problem. The caused by dengue virus (DENV), which member of the Flaviviridae family contains positive single-stranded RNA genome that encodes single precursor polyprotein further cleaved into structural non-structural proteins. Among these proteins, 3 (NS3) protein very because it forms non-covalent complex with NS2B cofactor, thereby forming functional viral protease. NS3 also C-terminal ATPase/helicase domain...

Background: A range of different neurological manifestations has been reported in fetuses and adults after Zika virus (ZIKV) infection. Objective: We describe a detection the ZIKV brain tissue from multiple sclerosis (MS) patient with acute disseminated encephalomyelitis (ADEM)-like event Rio de Janeiro, Brazil. Methods: Biological samples collected during hospitalization were tested by serology molecular diagnostic for various infectious agents. Histopathological analysis was performed...

Dengue virus (DENV) is an emerging involved in outbreaks Brazil. The association between the and vertical transmission, with disorders placenta, has raised a worldwide concern. On 29th gestational week, pregnant woman presented severe complications due to DENV infection leading maternal fetus death. Postmortem analysis of fetal organs demonstrated presence using reverse transcriptase polymerase chain reaction (RT-PCR) brain non-structural protein 3 (NS3) staining placenta several peripheral...

Zika virus (ZIKV) is an emerging involved in recent outbreaks Brazil. The association between the and Guillain-Barré syndrome (GBS) or congenital disorders has raised a worldwide concern. In this work, we investigated rare case, which was associated with GBS spontaneous retained abortion. Using specific anti-ZIKV staining, identified placenta (mainly Hofbauer cells) several fetal tissues, such as brain, lungs, kidneys, skin liver. Histological analyses of organs revealed different types...

In the large Zika virus (ZIKV) epidemic that occurred in Brazil 2015, intrauterine fetal exposure to ZIKV was associated with a significant risk of developing microcephaly and neurological disorders infected infants. ZIKV-associated disease has since been reported 24 countries Americas. At present, definitive evidence is lacking regarding co-exposure other viral infections whether coinfection impacts acquiring either infection or severity. Here, we provide both human immunodeficiency (HIV)...

Abstract Dengue is an important arboviral infection, causing a broad range symptom that varies from life-threatening mild illness to severe clinical manifestations. Recent studies reported the impairment of central nervous system (CNS) after dengue characteristic previously considered as atypical and underreported. However, little known about neuropathology associated dengue. Since animal models are tools for helping understand pathogenesis, including neurological damages, aim this work was...

Dengue is a mild flu-like arboviral illness caused by dengue virus (DENV) that occurs in tropical and subtropical countries. An increasing number of reports have been indicating also associated to neurological manifestations, however, little known regarding the neuropathogenesis disease. Here, using BALB/c mice intravenously infected with DENV-2 strain 66985, we demonstrated capable invading damaging host's central nervous system (CNS). Brain cerebellum animals revealed histological...

Zika virus (ZIKV) is an emergent arthropod-borne whose outbreak in Brazil has brought major public health problems. Infected individuals have different symptoms, including rash and pruritus, which can be relieved by the administration of antiallergics. In case pregnant women, ZIKV cross placenta infect fetus leading to congenital defects. We identified that mast cells placentae patients who had during pregnancy infected. This led our investigation on possible role a infection, using HMC-1...

Dengue viral (DENV) infections can lead to acute pancreatitis and associated tissue damage. This study examined the pancreas from two fatal cases of DENV for histopathological changes as well detection cytokines, other inflammatory mediators. Tissue sections were prepared examination by ultrastructural techniques. Sections non-infected individuals in parallel a control. The presence replication macrophages was detected co-staining proteins NS3 CD68 immunofluorescence. Immunohistochemistry...

Leishmaniasis remains a serious public health problem in developing countries without effective control, whether by vaccination or chemotherapy. Part of the failure leishmaniasis control is due to lack new less toxic and more drugs able eliminate both lesions parasite. Oxiranes derived from naphthoquinones now being assayed are promising for treatment this group diseases. The predicted pharmacokinetic properties toxicological profiles epoxy-α-lapachone epoxymethoxy-lawsone have been compared...

Dengue is an arboviral disease caused by dengue virus (DENV), which transmitted to humans Aedes aegypti mosquitoes. Infection DENV most commonly results in a mild flu-like illness; however, the has been increasingly associated with neurological symptomatology. This association draws attention further investigations on impact of infection host's central nervous system. Here, we analyzed brain samples three fatal cases that occurred 2002 during outbreak Rio de Janeiro, Brazil. Brain tissues...

Abstract Background The impact of COVID-19 pandemic in pregnant patients is an important emerging topic. Evidence transplacental transmission and typical histopathological alterations the placenta are controversial literature. Methods This was a prospective observational cohort multicenter study which we selected 23 placentas 21 that showed detection SARS-CoV-2 RNA by RT-PCR tissue described both morphological clinical characteristics. Immunohistochemistry performed to localize virus...

Dengue virus (DENV) infections may result in asymptomatic cases or evolve into a severe disease, which involves multiple organ failure. Renal involvement dengue can be potentially related to an increased mortality. Aiming better understand the role of DENV renal injury observed human fatal cases, post-mortem investigations were performed four DENV-4 autopsies during epidemics Brazil. Tissues submitted histopathology, immunohistochemistry, viral quantification, and characterization cytokines...

Dengue virus (DENV) infection represents a worldwide public health concern and can cause damage to multiple organs, including the kidney. In this work, we investigated histopathological changes caused by dengue along with detection of inflammatory mediators, cytokines, cell expression patterns in renal tissue three fatal cases children. Hematoxylin Eosin staining was performed analyze these changes. Immunohistochemistry allowed for immunological markers tissues that were quantified further...

Intrauterine transmission of the Chikungunya virus (CHIKV) during early pregnancy has rarely been reported, although vertical observed in newborns. Here, we report four cases spontaneous abortion women who became infected with CHIKV between 11th and 17th weeks pregnancy. Laboratorial confirmation infection was conducted by RT-PCR on a urine sample for one case, other three were detection IgM anti-CHIKV antibodies. Hematoxylin eosin (H&E) staining an electron microscopy assay allowed us...

Abstract This work presents a prepare method of bio‐foam constituted by sodium alginate, chitosan (CH), and glyphosate to form material be applied in controlled release herbicide. It was investigated the influence mixture pH on physicochemical properties. Kinetic studies adsorption were performed. The interactions between polysaccharides herbicide also investigated. bio‐foams evaluated for their mechanical morphological All samples presented swelling degree above 1000%. prepared at 6 higher...

Hypertension is characterized by persistent elevated blood pressure levels, one of the leading causes death in world. Renovascular hypertension represents most common cause secondary hypertension, and its progress associated with overactivation renin angiotensin aldosterone system (RAAS), causing systemic local changes. Aliskiren a renin-inhibiting drug that optimizes RAAS suppression. In this sense, objective present study was to analyze morphophysiology left kidney Wistar rats renovascular...

Chikungunya virus (CHIKV) is an arthropod-borne first isolated in Tanzania, Africa. The has spread to Asia as well South and Central America through infected Aedes mosquitoes. Vertical transmission may also occur, was documented during a chikungunya outbreak La Réunion Island 2005. Since then, some authors have been discussing the role of placenta maternal-fetal CHIKV transmission. infection characterized by fever, headache, rash, arthralgia. However, atypical manifestations clinical...

SARS-CoV-2 is a virus that belongs to the Betacoronavirus genus of Coronaviridae family. Other coronaviruses, such as SARS-CoV and MERS-CoV, were associated with complications in pregnant women. Therefore, this study aimed report clinical history five women infected (four symptomatic one asymptomatic who gave birth stillborn child) during COVID-19 pandemic. They between August 2020 January 2021, period which there was still no vaccination for Brazil. In addition, their placental alterations...

Although vertical transmission of CHIKV has been reported, little is known about the role placenta in this virus and effects infection on maternal-fetal interface. In work we investigated five placentas from pregnant women who became infected during gestational period. Four formalin-fixed paraffin-embedded samples (cases 1-4) were positive for by RT-PCR. One (case 5) had no test placenta, but RT-PCR serum mother baby, confirming transmission. The analyzed regarding histopathological...