A5085729866- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Redox biology and oxidative stress
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Vector-borne infectious diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer, Hypoxia, and Metabolism
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- Cytokine Signaling Pathways and Interactions
- Estrogen and related hormone effects
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Melanoma and MAPK Pathways
- Metabolism, Diabetes, and Cancer
- Retinoids in leukemia and cellular processes
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Outbreaks Research
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Insect Resistance and Genetics
- Synthetic Organic Chemistry Methods
- Metal complexes synthesis and properties
Robert H. Lurie Comprehensive Cancer Center of Northwestern University
2021-2024
Northwestern University
2020-2024
Medical College of Wisconsin
2020-2024
Universidade Federal do Rio de Janeiro
2011-2021
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2020
Chromatin structure is regulated through posttranslational modifications of histone variants that modulate transcription. Although highly homologous, display unique amino acid sequences associated with specific functions. Abnormal incorporation contributes to cancer initiation, therapy resistance, and metastasis. This study reports that, among its biologic functions, H3.1 serves as a chromatin redox sensor engaged by mitochondrial H2O2. In breast cells, the oxidation H3.1Cys96 promotes...
Dengue is one of the main public health concerns worldwide. Recent estimates indicate that over 390 million people are infected annually with dengue virus (DENV), resulting in thousands deaths. Among DENV nonstructural proteins, NS1 protein only whose function during replication still unknown. a 46- to 55-kDa glycoprotein commonly found as both membrane-associated homodimer and soluble hexameric barrel-shaped lipoprotein. Despite its role pathogenic process, essential for proper RNA...
ABSTRACT Dengue virus (DENV) infects millions of people worldwide and is a major public health problem. DENV nonstructural protein 1 (NS1) conserved glycoprotein that associates with membranes also secreted into the plasma in DENV-infected patients. The present study describes novel mechanism by which NS1 inhibits terminal complement pathway. We first identified regulator vitronectin (VN) as DENV2 binding partner using yeast two-hybrid system. This interaction was further assessed...
Emerging evidence identifies major contributions of platelets to inflammatory amplification in dengue, but the mechanisms infection-driven platelet activation are not completely understood. Dengue virus nonstructural protein-1 (DENV NS1) is a viral protein secreted by infected cells with recognized roles dengue pathogenesis, it remains unknown whether NS1 contributes phenotype platelets. This study shows that recombinant DENV activated toward an partially reproduced infection. stimulation...
DENV is one of the most relevant mosquito-transmitted viruses worldwide, infecting more than 390 million people every year and leading to 20 thousand deaths. Although a vaccine has already been approved, its potential side effects have hampered use in large-scale immunizations.
Dengue is an important mosquito-borne disease and a global public health problem. The caused by dengue virus (DENV), which member of the Flaviviridae family contains positive single-stranded RNA genome that encodes single precursor polyprotein further cleaved into structural non-structural proteins. Among these proteins, 3 (NS3) protein very because it forms non-covalent complex with NS2B cofactor, thereby forming functional viral protease. NS3 also C-terminal ATPase/helicase domain...
Besides antigen-specific responses to viral antigens, humoral immune response in virus infection can generate polyreactive and autoreactive antibodies. Dengue Zika infections have been linked antibody-mediated autoimmune disorders, including Guillain-Barré syndrome. A unique feature of flaviviruses is the secretion nonstructural protein 1 (NS1) by infected cells. NS1 highly immunogenic, antibodies targeting both protective pathogenic roles. In present study, we investigated found be an...
The non-structural 1 (NS1) protein plays an important role in dengue diagnosis because it has been detected as a soluble serum antigen both primary and secondary infections. NS1 was expressed Escherichia coli cells, the efficiency of four different refolding protocols tested. All generated dimeric conformation similar to that by eukaryotic cells. A polyclonal antibody produced from properly folded E. recombinant (rNS1) proved be useful tool for Dengue virus 100% 2 (DENV2) infected patients'...
Suppressor of cytokine signaling-1 (SOCS1) exerts control over inflammation by targeting p65 nuclear factor-κB (NF-κB) for degradation in addition to its canonical role regulating signaling. We report here that SOCS1 does not operate on all targets equally, instead localizing a select subset pro-inflammatory genes. Promoter-specific interactions and determine the genes activated NF-κB during systemic inflammation, with profound consequences responses, immune cell mobilization, tissue injury....
The dichotomous behavior of superoxide dismutase-2 (SOD2) in cancer biology has long been acknowledged and more recently linked to different posttranslational forms the enzyme. However, a distinctive activity underlying its tumor-promoting function is yet be described. Here, we report that acetylation, one such modifications (PTMs), increases SOD2 affinity for iron, effectively changing biochemical this enzyme from an antioxidant demethylase. Acetylated, iron-bound localizes nucleus,...
Abstract Colorectal cancer (CRC) remains a global health concern, constituting large portion of incidence and mortality. Despite advances in comprehending the genetic epigenetic landscape CRC progress achieved developing KRAS-specific inhibitors poised for clinical evaluation, significant unmet medical need persists. This underscores necessity innovative therapeutic interventions this field, as effective strategies targeting specific molecular vulnerabilities are currently lacking. Our study...
Bacterial pneumonia poses many challenges in ICU settings due to treatment resistance and the potential for lethal pulmonary injury sepsis. Neutrophil recruitment is crucial positive patient outcomes, but how achieve balance between effective microbial killing restrained injurious effector functions remains elusive. Our study aims investigate mechanisms underlying neutrophil functions, particularly explosive release of chromatin via NETosis, a process associated with poor outcomes various...
ABSTRACT Transcription stability enforces cellular identity and is tightly controlled by restrictions imposed on both transcription factor function target gene accessibility. Progression of cancer to metastasis multi-drug resistance requires fluid transcriptional programs that can explore different genomic landscapes enable clonal expansion aggressive treatment resistant phenotypes. Here, we show increased levels H 2 O produced in mitochondria leads H3.1 oxidation at Cys96, a distinctive...
Abstract Besides antigen-specific responses to viral antigens, humoral immune response in virus infection can generate polyreactive and autoreactive antibodies. Dengue Zika infections have been linked antibody-mediated autoimmune disorders including Guillain-Barrè syndrome. A unique feature of flaviviruses is the secretion non-structural protein 1 (NS1) by infected cells. NS1 highly immunogenic antibodies targeting both protective pathogenic roles. In present study, we investigated found be...