A5061008099- RNA Research and Splicing
- RNA modifications and cancer
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Advanced Proteomics Techniques and Applications
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Cancer Mechanisms and Therapy
- Acute Myeloid Leukemia Research
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Cancer-related Molecular Pathways
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Diet and metabolism studies
- Epigenetics and DNA Methylation
- Biotin and Related Studies
- Synthesis and Biological Evaluation
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Adipose Tissue and Metabolism
- Sarcoma Diagnosis and Treatment
- Inflammatory Bowel Disease
- Pancreatic and Hepatic Oncology Research
The Maria Sklodowska-Curie National Research Institute of Oncology
2016-2025
National Institute of Oncology
2022-2023
National Academy of Medical Sciences of Ukraine
2014-2020
State Institution "Institute for Children and Adolescents Health Care at the National Academy of Medical Sciences of Ukraine"
2013-2018
University of Washington
2011-2015
Union University
2015
Background Clinical progression of colorectal cancers (CRC) may occur in parallel with distinctive signaling alterations. We designed multidirectional analyses integrating microarray-based data biostatistics and bioinformatics to elucidate the metabolic alterations underlying CRC development adenoma-carcinoma sequence. Methodology/Principal Findings Studies were performed on normal mucosa, adenoma, carcinoma samples obtained during surgery or colonoscopy. Collections cryostat sections...
Abstract Here we present the Transcription Factor Encyclopedia (TFe), a new web-based compendium of mini review articles on transcription factors (TFs) that is founded principles open access and collaboration. Our consortium over 100 researchers has collectively contributed 130 pertinent human, mouse rat TFs. Notable features TFe website include high-quality PDF generator web API for programmatic data retrieval. aims to rapidly educate scientists about TFs they encounter through delivery...
Little data are available on the subject of gut microbiota composition in endurance athletes as well connections between diet and specific bacteria abundance. However, most studies suggest that athletes' undergoes major alterations, which may contribute to increased physical performance. Therefore, we decided investigate differences healthy controls athletes.Stools samples were collected from 14 marathon runners, 11 cross-country skiers 46 sedentary controls. The evaluation was performed...
Histone post-translational modifications (PTMs) play an important role in the regulation of expression genes, including those involved cancer development and progression. However, our knowledge PTM patterns human tumours is limited.MS-based analyses were used to quantify global alterations histone PTMs colorectal (CRC) samples. Histones isolated from 12 CRCs their corresponding normal mucosa by acidic extraction separated SDS-PAGE analysed liquid chromatography-mass spectrometry.Among 96...
Inhibition of oncogenic transcriptional programs is a promising therapeutic strategy. A substituted tricyclic benzimidazole, SEL120-34A, novel inhibitor Cyclin-dependent kinase 8 (CDK8), which regulates transcription by associating with the Mediator complex. X-ray crystallography has shown SEL120-34A to be type I forming halogen bonds protein's hinge region and hydrophobic complementarities within its front pocket. inhibits phosphorylation STAT1 S727 STAT5 S726 in cancer cells vitro....
We analysed critically the potential usefulness of RNA- and DNA-based biomarkers in supporting conventional histological diagnostic tests for prostate carcinoma (PCa) detection. Microarray profiling gene expression DNA methylation was performed on 16 benign prostatic hyperplasia (BPH) 32 cancerous non-cancerous samples extracted by radical prostatectomy. The predictive value selected validated qPCR-based methods using tissue from 58 prostates and, separately, 227 core biopsies. HOXC6, AMACR...
Irritable bowel syndrome (IBS) is a chronic functional disorder and its development may be linked, directly indirectly, to intestinal dysbiosis. Here we investigated the interactions between IBS symptoms gut microbiome, including relation rifaximin (1200 mg daily; 11.2 g per treatment). We recruited 72 patients, 31 with IBS-D (diarrhea), 11 IBS-C (constipation), 30 IBS-M (mixed constipation diarrhea) healthy controls (HCs). Of them, 68%, 64%, 53% patients IBS-D, IBS-C, IBS-M, respectively,...
Epithelial tissues achieve a highly organized structure due to cell-cell junction complexes. Carcinogenesis is accompanied by changes in cell interactions and tissue morphology, which appear the early stages of benign tumors progress along with invasive potential. The aim present study was analyze expression levels genes encoding intercellular proteins that have been previously identified be differentially expressed colorectal compared normal mucosa samples (fold change, >2.5) genome-wide...
Background. Recent studies have shown that isocitrate dehydrogenase 1/2 (IDH1/2)- activating mutations occur in a variety of cancers, including acute myeloid leukaemia, gliomas, and chondrosarcomas (CHS)s. The effect IDH1/2 mutation on overall survival (OS) has not been reported CHS. aim our study was to assess the prevalence known cancer-related gene CHS, as well their prognostic role patient survival. Methods. DNA from FFPE samples 80 patients (F:M- 1:1.3; mean age: 58 years; range 27-86)...
Aging affects iron homeostasis, as evidenced by tissue loading and anemia in the elderly. Iron needs mammals are met primarily recycling from senescent red blood cells (RBCs), a task chiefly accomplished splenic pulp macrophages (RPMs) via erythrophagocytosis. Given that RPMs continuously process iron, their cellular functions might be susceptible to age-dependent decline, possibility has been unexplored date. Here, we found 10- 11-month-old female mice exhibit RPMs, largely attributable...
The heterogeneous nuclear ribonucleoprotein K is an ancient RNA/DNA-binding protein that involved in multiple processes compose gene expression. pleiotropic action of reflects its ability to interact with different classes factors, interactions are regulated by extracellular signals. We used affinity purification and MS better define the repertoire partners. identified a large number new partners, some typically found subcellular compartments, such as plasma membrane, where has not...
Background: Clinical progression of colorectal cancers (CRC) may occur in parallel with distinctive signaling alterations.We designed multidirectional analyses integrating microarray-based data biostatistics and bioinformatics to elucidate the metabolic alterations underlying CRC development adenoma-carcinoma sequence.Methodology/Principal Findings: Studies were performed on normal mucosa, adenoma, carcinoma samples obtained during surgery or colonoscopy.Collections cryostat sections...
Transplanting a fecal sample from lean, healthy donors to obese recipients has been shown improve metabolic syndrome symptoms. We therefore examined the gut microbiota in mice after administering long-term, high-fat diet (HFD) supplemented with feces lean through fecal-oral route. C57BL6/W were allowed adapt non-specific pathogen free (SFP) environment for 2 weeks before being divided into three groups of 16 animals. Animals fed 28 normal (ND), HFD or ND-fed (HFDS). The composition...
Obesity contributes to the development of non‑alcoholic fatty liver disease (NAFLD), which is characterized by upregulated expression two key inflammatory mediators: tumor necrosis factor (Tnfa) and monocyte chemotactic protein 1 (Mcp1; also known as Ccl2). However, chromatin make-up at these genes in obese individuals has not been explored. In this study, identify obesity-mediated epigenetic changes Tnfa Ccl2, we used a murine model obesity induced high-fat diet (HFD) hyperphagic (ob/ob)...
The Tie2/angiopoietin (Tie2/Ang) and vascular endothelial growth factor receptor-ligand systems (VEGFR/VEGF) are recognized to play important roles in the regulation of microvascular function. Downregulation these genes during sepsis has been implicated pathogenesis sepsis-related leak multiple organ dysfunction syndrome. Mechanisms responsible for dysregulation angiogenic poorly defined.Western blot, reverse transcription-polymerase chain reaction, multiplex chromatin immunoprecipitation...
Burkitt lymphoma (BL) is a rapidly growing tumor, characterized by high anabolic requirements. The MYC oncogene plays central role in the pathogenesis of this malignancy, controlling genes involved apoptosis, proliferation, and cellular metabolism. Serine biosynthesis pathway (SBP) couples glycolysis to folate methionine cycles, supporting certain amino acids, nucleotides, glutathione, methyl group donor, S-adenosylmethionine (SAM). We report that BLs overexpress SBP enzymes,...
Growing tumors avoid recognition and destruction by the immune system. During continuous stimulation of tumor-infiltrating lymphocytes (TILs) tumors, TILs become functionally exhausted; thus, they unable to kill tumor cells produce certain cytokines lose their ability proliferate. This collectively results in escape cancer cells. Here, we show that breast expressing PD-L1 can accelerate exhaustion persistently activated human effector CD4+ T cells, manifesting high PD-1 expression level son...
Esports is a category of competitive video games that, in many aspects, may be similar to traditional sports; however, the gut microbiota composition players has not been yet studied.
Abstract Background & Aims Wilson's disease (WD) is an autosomal recessive disorder associated with disease‐causing alterations across the ATP7B gene, highly variable symptoms and age of onset. We aimed to assess whether clinical variability WD relates modifier genes. Methods A total 248 patients were included, whom 148 diagnosed after 17. Human exome libraries constructed using AmpliSeq technology sequenced IonProton platform. Results p.His1069Gln mutation was present in 215 patients,...
Article13 January 2020Open Access Source DataTransparent process Synthetic lethality between VPS4A and VPS4B triggers an inflammatory response in colorectal cancer Ewelina Szymańska Corresponding Author [email protected] orcid.org/0000-0001-7994-0764 Laboratory of Cell Biology, International Institute Molecular Warsaw, Poland Search for more papers by this author Paulina Nowak Krzysztof Kolmus Magdalena Cybulska Department Genetics, Maria Skłodowska-Curie Institute-Oncology Centre, Goryca...