A5022206650- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Phagocytosis and Immune Regulation
- Ubiquitin and proteasome pathways
- Calcium signaling and nucleotide metabolism
- Retinal Development and Disorders
- Lipid Membrane Structure and Behavior
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Microtubule and mitosis dynamics
- Pancreatitis Pathology and Treatment
- Cancer-related Molecular Pathways
- Metabolism, Diabetes, and Cancer
- Peptidase Inhibition and Analysis
- Cell death mechanisms and regulation
- NF-κB Signaling Pathways
- Lysosomal Storage Disorders Research
- Immune Cell Function and Interaction
- Receptor Mechanisms and Signaling
- Signaling Pathways in Disease
- Advanced Fluorescence Microscopy Techniques
- interferon and immune responses
International Institute of Molecular and Cell Biology
2016-2025
Max Planck Institute of Molecular Cell Biology and Genetics
1999-2024
Max Planck Society
2002-2011
University of Vienna
1994-2001
European Molecular Biology Laboratory
2000
Vienna Biocenter
1994-1998
European Molecular Biology Organization
1997
Rab5 regulates endocytic membrane traffic by specifically recruiting cytosolic effector proteins to their site of action on early endosomal membranes. We have characterized a new complex involved in fusion events. This includes novel protein, Rabenosyn-5, which, like the previously endosome antigen 1 (EEA1), contains an FYVE finger domain and is recruited phosphatidylinositol-3-kinase–dependent fashion endosomes. Rabenosyn-5 complexed Sec1-like protein hVPS45. hVPS45 does not interact...
Rab GTPases are central elements of the vesicular transport machinery. An emerging view is that downstream effectors these multiprotein complexes include nucleotide exchange factors to ensure coupling between GTPase activation and effector function. We have previously shown Rab5, which regulates various steps along early endocytic pathway, activated by a complex consisting Rabex-5, Rab5 factor, Rabaptin-5. postulated physical association two proteins necessary for their activity in...
PI 3-kinases are important regulators of endocytic trafficking. We have previously proposed a model in which the Rab5 GTPase recruits EEA1 to early endosome both directly, by binding EEA1, and indirectly, through recruitment p150/hVps34 3-kinase production PI[3]P endosomal membrane. In this study we examined vivo. find that endogenous hVps34 p150 targeted enlarged structures cells expressing constitutively activated Rab5, where they significantly colocalized with EEA1. Recombinant fragments...
Endocytosis allows cargo to enter a series of specialized endosomal compartments, beginning with early endosomes harboring Rab5 and its effector EEA1. There are, however, additional structures labeled by the APPL1 whose role in endocytic transport remains unclear. It has been proposed that vesicles are intermediates convert into EEA1 endosomes. Here, we tested this model analyzing ultrastructural morphology, kinetics transport, stability compartment over time. We found resides on...
AXL, a member of the TAM (TYRO3, MER) receptor tyrosine kinase family, and its ligand, GAS6, are implicated in oncogenesis metastasis many cancer types. However, exact cellular processes activated by GAS6-AXL remain largely unexplored. Here, we identified an interactome AXL revealed associations with proteins regulating actin dynamics. Consistently, GAS6-mediated activation triggered remodeling manifested peripheral membrane ruffling circular dorsal ruffles (CDRs). This further promoted...
The molecular mechanisms ensuring directionality of endocytic membrane trafficking between transport vesicles and target organelles still remain poorly characterized. We have been investigating the function small GTPase Rab5 in early transport. <i>In vitro</i> studies demonstrated a role two fusion events: heterotypic plasma membrane-derived clathrin-coated (CCVs) endosomes homotypic endosomes. Several effectors are required endosome fusion, including EEA1, which mediates docking, as well...
Because signaling mediated by the transcription factor nuclear κB (NF-κB) is initiated ligands and receptors that can undergo internalization, we investigated how endocytic trafficking regulated this key physiological pathway. We depleted all of ESCRT (endosomal sorting complexes required for transport) subunits, which mediate receptor degradation, found components Tsg101, Vps28, UBAP1, CHMP4B were essential to restrict constitutive NF-κB in human embryonic kidney 293 cells. In absence...
A recent EMBO-FEBS workshop entitled Endocytic Systems: Mechanism and Function, organized by Howard Riezman in Villars-sur-Ollon (Switzerland), showcased the multifaceted approaches model systems used to study endocytosis. The meeting revealed how endocytosis controls multiple aspects of biology, ranging from development immunity neurotransmission.
ABSTRACT Platelet-derived growth factor receptor β (PDGFRβ) is a tyrosine kinase which upon activation by PDGF-BB stimulates cell proliferation, migration and angiogenesis. Ligand binding induces intracellular signaling cascades but also internalization of the receptor, eventually resulting in its lysosomal degradation. However, endocytic trafficking receptors often modulates their downstream signaling. We previously reported that PDGFRβ occurs via dynamin-dependent -independent pathways...
Article13 January 2020Open Access Source DataTransparent process Synthetic lethality between VPS4A and VPS4B triggers an inflammatory response in colorectal cancer Ewelina Szymańska Corresponding Author [email protected] orcid.org/0000-0001-7994-0764 Laboratory of Cell Biology, International Institute Molecular Warsaw, Poland Search for more papers by this author Paulina Nowak Krzysztof Kolmus Magdalena Cybulska Department Genetics, Maria Skłodowska-Curie Institute-Oncology Centre, Goryca...